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Insights into the genetic basis of the pre-breeding potato clones developed at the Julius Kühn Institute for high and durable late blight resistance

Published online by Cambridge University Press:  08 September 2021

Johanna Blossei Open the ORCID record for Johanna Blossei [Opens in a new window] ,
Ralf Uptmoor ,
Ramona Thieme ,
Marion Nachtigall  and
Thilo Hammann
Johanna Blossei*
Affiliation:
Julius Kühn Institute (JKI) – Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Agricultural Crops, Germany
Ralf Uptmoor
Affiliation:
Chair of Agronomy, Faculty of Agriculture and Environmental Science, University of Rostock, Rostock, Germany
Ramona Thieme
Affiliation:
Julius Kühn Institute (JKI) – Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Agricultural Crops, Germany
Marion Nachtigall
Affiliation:
Julius Kühn Institute (JKI) – Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Agricultural Crops, Germany
Thilo Hammann
Affiliation:
Julius Kühn Institute (JKI) – Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Agricultural Crops, Germany
*
Author for correspondence: Johanna Blossei, E-mail: johanna.blossei@julius-kuehn.de
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Abstract

Due to the high yield losses caused by late blight in potato cultivation, the development of resistant pre-breeding material is of great importance for cultivar breeding. The gene pool of the Julius Kühn Institute (JKI) includes a large collection of resistant clones whose resistance has not yet been analysed in detail with markers for relevant resistance genes. A panel of 52 pre-breeding potato clones developed at the JKI via interspecific crosses and highly resistant to late blight were tested for the presence of seven resistance genes (Rpi-blb1/Rpi-sto1, Rpi-blb2, Rpi-blb3/R2/Rpi-abpt, R1, R3a, R3b, Rpi-phu1) and one QTL allele (QTL_phu-stn) from Solanum species S. bulbocastanum, S. demissum, S. phureja and S. stoloniferum, respectively. Molecular marker assays based on sequence-specific primers revealed that 36 of the 52 pre-breeding clones carried either 1, 2, 3 or 4 resistance genes introgressed from these wild Solanum species. Results indicate that these resistance genes were retained over generations of breeding. Although highly resistant to late blight, 16 pre-breeding clones did not carry any of these resistance genes. Resistance in the gene pool may, thus, be based not only on individual resistance genes but also on QTL effects. Results help to better understand both inheritance and expression of late blight resistance of this unique gene pool and may be used for breeding programmes.

Keywords

Crop wild relativesgene poollate blight (Phytophthora infestans)potato (Solanum tuberosum L.)resistance genes
Type
Short Communication
Information
Plant Genetic Resources , Volume 19 , Issue 5 , October 2021 , pp. 461 - 464
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of NIAB

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