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Molecular characterization of 5S ribosomal RNA genes and transcripts in the protozoan parasite Leishmania major

Published online by Cambridge University Press:  06 October 2016

RODRIGO MORENO-CAMPOS ,
LUIS E. FLORENCIO-MARTÍNEZ ,
TOMÁS NEPOMUCENO-MEJÍA ,
SAÚL ROJAS-SÁNCHEZ ,
DANIEL E. VÉLEZ-RAMÍREZ ,
NORMA E. PADILLA-MEJÍA ,
ELISA FIGUEROA-ANGULO ,
REBECA MANNING-CELA  and
SANTIAGO MARTÍNEZ-CALVILLO
RODRIGO MORENO-CAMPOS
Affiliation:
Unidad de Biomedicina, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Av. De los Barrios 1, Col. Los Reyes Iztacala, Tlalnepantla, Edo. de México, CP 54090, México
LUIS E. FLORENCIO-MARTÍNEZ
Affiliation:
Unidad de Biomedicina, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Av. De los Barrios 1, Col. Los Reyes Iztacala, Tlalnepantla, Edo. de México, CP 54090, México
TOMÁS NEPOMUCENO-MEJÍA
Affiliation:
Unidad de Biomedicina, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Av. De los Barrios 1, Col. Los Reyes Iztacala, Tlalnepantla, Edo. de México, CP 54090, México
SAÚL ROJAS-SÁNCHEZ
Affiliation:
Unidad de Biomedicina, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Av. De los Barrios 1, Col. Los Reyes Iztacala, Tlalnepantla, Edo. de México, CP 54090, México
DANIEL E. VÉLEZ-RAMÍREZ
Affiliation:
Unidad de Biomedicina, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Av. De los Barrios 1, Col. Los Reyes Iztacala, Tlalnepantla, Edo. de México, CP 54090, México
NORMA E. PADILLA-MEJÍA
Affiliation:
Unidad de Biomedicina, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Av. De los Barrios 1, Col. Los Reyes Iztacala, Tlalnepantla, Edo. de México, CP 54090, México
ELISA FIGUEROA-ANGULO
Affiliation:
Unidad de Biomedicina, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Av. De los Barrios 1, Col. Los Reyes Iztacala, Tlalnepantla, Edo. de México, CP 54090, México
REBECA MANNING-CELA
Affiliation:
Departamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados del IPN, Av. IPN 2508, México, D.F., CP 07360, México
SANTIAGO MARTÍNEZ-CALVILLO*
Affiliation:
Unidad de Biomedicina, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Av. De los Barrios 1, Col. Los Reyes Iztacala, Tlalnepantla, Edo. de México, CP 54090, México
*
*Corresponding author: Unidad de Biomedicina, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Av. De los Barrios 1, Col. Los Reyes Iztacala, Tlalnepantla, Edo. de México, CP 54090, México. E-mail: scalv@campus.iztacala.unam.mx
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Summary

Eukaryotic 5S rRNA, synthesized by RNA polymerase III (Pol III), is an essential component of the large ribosomal subunit. Most organisms contain hundreds of 5S rRNA genes organized into tandem arrays. However, the genome of the protozoan parasite Leishmania major contains only 11 copies of the 5S rRNA gene, which are interspersed and associated with other Pol III-transcribed genes. Here we report that, in general, the number and order of the 5S rRNA genes is conserved between different species of Leishmania. While in most organisms 5S rRNA genes are normally associated with the nucleolus, combined fluorescent in situ hybridization and indirect immunofluorescence experiments showed that 5S rRNA genes are mainly located at the nuclear periphery in L. major. Similarly, the tandemly repeated 5S rRNA genes in Trypanosoma cruzi are dispersed throughout the nucleus. In contrast, 5S rRNA transcripts in L. major were localized within the nucleolus, and scattered throughout the cytoplasm, where mature ribosomes are located. Unlike other rRNA species, stable antisense RNA complementary to 5S rRNA is not detected in L. major.

Keywords

5S rRNAPol III transcriptionLeishmaniagene expression
Type
Research Article
Information
Parasitology , Volume 143 , Issue 14 , December 2016 , pp. 1917 - 1929
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Copyright
Copyright © Cambridge University Press 2016 

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