Skip to main content Accessibility help

The interaction of human eosinophils and neutrophils with non-phagocytosable surfaces: a model for studying cell-mediated immunity in schistosomiasis

  • Audrey M. Glauert (a1), Rhonda C. Oliver (a1) and Kareen J. I. Thorne (a1)


A model has been developed to simulate the surface of an antibody-coated schistosomulum. It consists of a layer of agar, containing antigen (tetanus toxoid) and a chemotactic factor (ECF). Some layers were coated with human anti-tetanus immunoglobulin. The mode of adherence of human eosinophils and neutrophils to these agar layers and the subsequent degranulation of the cells exactly paralleled the interaction of these cell types with antibody-coated schistosomula of Schistosoma mansoni. In particular, eosinophils made much more intimate contact than did neutrophils, and lysosomal enzymes were secreted extracellularly by direct fusion of granules with the plasma membrane of the cell. Biochemical evidence was also obtained for the secretion of enzymes during degranulation and the rate of enzyme release was found to be enhanced in the presence of specific antibody. This model, non-phagocytosable surface has the potential to provide basic information on the mode of action of effector cells in cell-mediated cytotoxic reactions against a wide range of parasites by incorporation of different factors into the agar layers.



Hide All
Anwar, A. R. E., Smithers, S. R. & Kay, A. B. (1979). Killing of schistosomula of Schistosoma mansoni coated with antibody and/or complement by human leukocytes in vitro: requirement for complement in preferential killing by eosinophils. Journal of Immunology 122, 628–37.
Archer, G. T. & Hirsch, J. G. (1963). Motion picture studies on degranulation of horse eosinophils during phagocytosis. Journal of Experimental Medicine 118, 287–94.
Becker, E. L. & Henson, P. M. (1973). In vitro studies of immunologically induced secretion of mediators from cells and related phenomena. Advances in Immunology 17, 93193.
Butterworth, A. E. (1977). Effector mechanisms against schistosomes in vitro. American Journal of Tropical Medicine and Hygiene 26(6), Suppl. 2938.
Butterworth, A. E., Wassom, D. L., Gleich, G. J., Loegering, D. A. & David, J. R. (1979). Damage to schistosomula of Schistosoma mansoni induced directly by eosinophil major basic protein. Journal of Immunology 122, 221–9.
Capron, M., Capron, A., Torpier, G., Bazin, H., Bout, D. & Joseph, M. (1978). Eosinophil-dependent cytotoxicity in rat schistosomiasis. Involvement of IgG2a antibody and role of mast cells. European Journal of Immunology 8, 127–33.
Densen, P., Mahmoud, A. A. F., Sullivan, J., Warren, K. S. & Mandell, G. L. (1978). Demonstration of eosinophil degranulation on the surface of opsonized schistosomules by phase-contrast cinemicrography. Infection and Immunity 22, 282–5.
Djerassi, I., Kim, J. S., Suvansri, U., Mitrakul, C. & Ciesielka, W. (1972). Continuous flow-filtration leukapheresis. Transfusion 12, 7583.
Glauert, A. M. & Butterworth, A. E. (1977). Morphological evidence for the ability of eosinophils to damage antibody-coated schistosomula. Transactions of the Royal Society of Tropical Medicine and Hygiene 71, 392–5.
Glauert, A. M., Butterworth, A. E., Sturrock, R. F. & Houba, V. (1978). The mechanism of antibody-dependent, eosinophil-mediated damage to schistosomula of Schistosoma mansoni in vitro: a study by phase-contrast and electron microscopy. Journal of Cell Science 34, 173–92.
Goetzl, E. J. & Austen, K. F. (1975). Purification and synthesis of eosinophilotactic tetra-peptides of human lung tissue: identification as eosinophil chemotactic factor of anaphylaxis. Proceedings of the National Academy of Sciences, USA 72, 4123–7.
Hawkins, D. (1971). Biopolymer membrane: a model system for the study of the neutrophilic leukocyte response to immune complexes. Journal of Immunology 107, 344–52.
Henson, P. M. (1971 a). The immunologic release of constituents from neutrophil leukocytes. I. The role of antibody and complement on nonphagocytosable surfaces or phagocytosable particles. Journal of Immunology 107, 1535–46.
Henson, P. M. (1971 b). The immunologic release of constituents from neutrophil leukocytes. II. Mechanisms of release during phagocytosis, and adherence to nonphagocytosable surfaces. Journal of Immunology 107, 1547–57.
Houba, V., Butterworth, A. E., David, J. R., Sher, A., Glauert, A. M., Sturrock, R. F. & Vadas, M. A. (1979). Lysosomes in immunity to schistosomes and other helminths. In Lysoaomes in Biology and Pathology, vol. 6 (ed. Dingle, J. T, Jacques, P. and Shaw, I. H), pp. 329. Amsterdam: North-Holland.
Kay, A. B. & Austen, K. F. (1971). The IgE-mediated release of an eosinophil leukocyte chemotactic factor from human lung. Journal of Immunology 107, 899902.
Kazura, J. W. & Grove, D. I. (1978). Stage-specific antibody-dependent eosinophil-mediated destruction of Trichinella spiralis. Nature, London 274, 588–9.
Klebanoff, S. J. (1975). Antimicrobial mechanisms in neutrophil polymorphonuclear leukocytes. Seminars in Haematology 12, 117–42.
Lackie, J. M. & De Bono, D. (1977). Interactions of neutrophil granulocytes (PMNs) and endothelium in vitro. Microvascular Research 13, 107–12.
Mclaren, D. J., Mackenzie, C. D. & Ramalho-Pinto, F. J. (1977). Ultrastructural observations on the in vitro interaction between rat eosinophils and some parasitic helminths (Schistosoma mansoni, Trichinella spiralis and Nippostrongylus brasiliensis). Clinical and Experimental Immunology 30, 105–18.
McLaren, D. J., Ramalho-Pinto, F. J. & Smithers, S. R. (1978). Ultrastructural evidence for complement and antibody-dependent damage to schistosomula of Schistosoma mansoni by rat eosinophils in vitro. Parasitology 77, 313–24.
Migler, R. & Dechatelet, L. R. (1978). Human eosinophilic peroxidase: biochemical characterisation. Biochemical Medicine 19, 1626.
Murphy, G., Reynolds, J. J., Bretz, U. & Baggiolini, M. (1977). Collagenase is a component of the specific granules of human neutrophil leucocytes. Biochemical Journal 162, 195–7.
Ogilvie, B. M., Mackenzie, C. D. & Love, R. J. (1977). Lymphocytes and eosinophils in the immune response of rats to initial and subsequent infections with Nippostrongylus brasiliensis. American Journal of Tropical Medicine and Hygiene 26(6), Suppl. 61–7.
Ohlsson, K. & Olsson, I. (1977). The extracellular release of granulocyte collagenase and elastase during phagocytosis and inflammatory processes. Scandinavian Journal of Haematology 19, 145–52.
Smithers, S. R. & Terry, R. J. (1976). The immunology of schistosomiasis. Advances in Parasitology 14, 399422.
Thorne, K. J. I., Oliver, R. C. & Glauert, A. M. (1980). Eosinophil interaction with antibody-coated, non-phagocytosable surfaces: changes in cell surface proteins. Journal of Cell Science (in the Press).
Vadas, M. A., David, J. R., Butterworth, A. E., Pisani, N. T. & Siongok, T. A. (1979). A new method for the purification of human eosinophils and neutrophils, and a comparison of the ability of these cells to damage schistosomula of Schistosoma mansoni. Journal of Immunology 122, 1228–36.
von Lichtenberg, F. (1977). Experimental approaches to human schistosomiasis. American Journal of Tropical Medicine and Hygiene 26(6), Suppl. 7987.
von Lichtenberg, F., Sher, A., Gibbons, N. & Doughty, B. L. (1976). Eosinophil-enriched inflammatory response to schistosomula in the skin of mice immune to Schistosoma mansoni. American Journal of Pathology 84, 479500.

The interaction of human eosinophils and neutrophils with non-phagocytosable surfaces: a model for studying cell-mediated immunity in schistosomiasis

  • Audrey M. Glauert (a1), Rhonda C. Oliver (a1) and Kareen J. I. Thorne (a1)


Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed.