Skip to main content Accessibility help
×
Home
Hostname: page-component-684bc48f8b-2l47r Total loading time: 0.372 Render date: 2021-04-11T17:21:03.992Z Has data issue: true Feature Flags: { "shouldUseShareProductTool": true, "shouldUseHypothesis": true, "isUnsiloEnabled": true, "metricsAbstractViews": false, "figures": false, "newCiteModal": false, "newCitedByModal": true }

Article contents

Molecular characterization of Fasciola jacksoni from wild elephants (Elephas maximus maximus) of Sri Lanka: a taxonomic evaluation

Published online by Cambridge University Press:  24 June 2019

R. P. V. J. Rajapakse
Affiliation:
Department of Veterinary Pathobiology, Faculty of Veterinary Medicine and Animal Science, University of Peradeniya, Peradeniya, Sri Lanka
S. P. Lawton
Affiliation:
Molecular Parasitology Laboratory, School of Life Sciences, Pharmacy and Chemistry, Kingston University London, Kingston Upon Thames, Surrey, KT12 EE, 32, UK
K. J. K. Karunathilake
Affiliation:
Department of Veterinary Pathobiology, Faculty of Veterinary Medicine and Animal Science, University of Peradeniya, Peradeniya, Sri Lanka
B. V. P. Perera
Affiliation:
Department of Wildlife Conservation, Elephant Trust Home, Udawalave 70190, Sri Lanka
N. T. B. Nguyen
Affiliation:
Institute of Biotechnology (IBT), Vietnam Academy of Science and Technology (VAST), 18. Hoang Quoc Viet Rd., Cau Giay, Hanoi, Vietnam
T. H. Le
Affiliation:
Institute of Biotechnology (IBT), Vietnam Academy of Science and Technology (VAST), 18. Hoang Quoc Viet Rd., Cau Giay, Hanoi, Vietnam Graduate University of Science and Technology (GUST), Vietnam Academy of Science and Technology (VAST), 18. Hoang Quoc Viet Rd., Cau Giay, Hanoi, Vietnam
Corresponding
E-mail address:

Abstract

Fasciola jacksoni is a significant contributor to the health and mortality of Asian elephants, particularly those in Sri Lanka. Despite the impact of fascioliasis on elephant populations, it is a neglected veterinary disease with limited taxonomic understanding. Molecular characterization and phylogenetic analysis of F. jacksoni were carried out to evaluate its suggested basal position in the Fasciolidae. Adult worms were collected during post-mortem of elephants, and eggs were collected from living elephants in National parks across Sri Lanka. Using the mitochondrial genes nicotinamide dehydrogenase subunit 1 (nad1) and cytochrome oxidase subunit 1 (cox1), and a partial 28S ribosomal DNA (28S rDNA), DNA sequences were generated from the F. jacksoni adult and egg material. Maximum likelihood (ML) phylogenetic analyses did not resolve F. jacksoni to be basal to the Fasciolidae. Furthermore, the ML analyses showed that the genus Fasciola was not monophyletic and that F. jacksoni was a sister species to the deer liver fluke Fascioloides magna. A clear framework is required to determine the taxonomic status of F. jacksoni and this current study provides the first detailed application of molecular techniques from multiple hosts across Sri Lanka with the production of reference DNA sequences for this important parasite.

Type
Research Article
Copyright
Copyright © Cambridge University Press 2019 

Access options

Get access to the full version of this content by using one of the access options below.

References

Alahakoon, J (1994) Presence of liver fluke Fasciola jacksoni in a Sri Lankan elephant. Gajah 12, 4647.Google Scholar
Bhalerao, GD (1933) The trematode parasites of the Indian elephant, Elephas indicus. Indian Journal of Veterinary Science and Animal Husbandry 3, 103155.Google Scholar
Caple, IW, Jainudeen, MR, Buick, TD and Song, CY (1978) Some clinico-pathologic findings in elephants (Elephas maximus) infected with Fasciola jacksoni. Journal of Wildlife Diseases 14, 110115.CrossRefGoogle ScholarPubMed
Cobbold, T. S. (1869). Fasciola jacksoni. Quarterly Journal of Microscopical Science; Supplement to Entozoa. p. 80.Google Scholar
Cobbold, TS (1882) VII. The parasites of elephants. Transactions of the Linnean Society of London. 2nd Series: Zoology 2, 223258.CrossRefGoogle Scholar
Fernando, P (2000) Elephants in Sri Lanka: past present and future. Loris 22, 3844.Google Scholar
Fernando, P, Wikramanayake, E, Weerakoon, D, Jayasinghe, L, Gunawardene, M and Janaka, H (2005) Perceptions and patterns of human–elephant conflict in old and new settlements in Sri Lanka: insights for mitigation and management. Biodiversity and Conservation 14, 24652481.CrossRefGoogle Scholar
Heneberg, P (2013) Phylogenetic data suggest the reclassification of Fasciola jacksoni (Digenea: Fasciolidae) as Fascioloides jacksoni comb. nov. Parasitology Research 112, 16791689.CrossRefGoogle ScholarPubMed
Hing, S, Othman, N, Nathan, S, Fox, M, Fisher, M and Goossens, B (2013) First parasitological survey of Endangered Bornean elephants Elephas maximus borneensis. Endangered Species Research 21, 223230.CrossRefGoogle Scholar
Hing, S, Narayan, EJ, Thompson, RCA and Godfrey, SS (2016) The relationship between physiological stress and wildlife disease: consequences for health and conservation. Wildlife Research 43, 51.CrossRefGoogle Scholar
Islam, S (1997) Studies on some aspects of fascioliasis in Asian elephants (Elephas maximus). Journal of Veterinary Parasitology 11, 109.Google Scholar
Králová-Hromadová, I, Špakulová, M, Horáčková, E, Turčeková, L, Novobilský, A, Beck, R, Koudela, B, Marinculić, A, Rajský, D and Pybus, M (2008) Sequence analysis of ribosomal and mitochondrial genes of the giant liver fluke Fascioloides magna (Trematoda: Fasciolidae): intraspecific variation and differentiation from Fasciola hepatica. Journal of Parasitology 94, 5867.CrossRefGoogle ScholarPubMed
Kumar, S, Stecher, G and Tamura, K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 33, 18701874.CrossRefGoogle ScholarPubMed
Le, TH, De, NV, Agatsuma, T, Nguyen, TGT, Nguyen, QD, Mcmanus, DP and Blair, D (2008) Human fascioliasis and the presence of hybrid/introgressed forms of Fasciola hepatica and Fasciola gigantica in Vietnam. International Journal for Parasitology 38, 725730.CrossRefGoogle ScholarPubMed
Le, TH, Nguyen, KT, Nguyen, NTB, Doan, HTT, Dung, DT and Blair, D (2017) The ribosomal transcription units of Haplorchis pumilio and H. taichui and the use of 28S rDNA sequences for phylogenetic identification of common heterophyids in Vietnam. Parasites & Vectors 10. doi: 10.1186/s13071-017-1968-0.CrossRefGoogle ScholarPubMed
Liu, G-H, Gasser, RB, Young, ND, Song, H-Q, Ai, L and Zhu, X-Q (2014) Complete mitochondrial genomes of the ‘intermediate form’ of Fasciola and Fasciola gigantica, and their comparison with F. hepatica. Parasites & Vectors 7, 150.CrossRefGoogle ScholarPubMed
Lorimer, J (2010) Elephants as companion species: the lively biogeographies of Asian elephant conservation in Sri Lanka. Transactions of the Institute of British Geographers 35, 491506.CrossRefGoogle Scholar
Lotfy, WM, Brant, SV, DeJong, RJ, Le, TH, Demiaszkiewicz, A, Rajapakse, RPVJ, Perera, VBVP, Laursen, JR and Loker, ES (2008) Evolutionary origins, diversification, and biogeography of liver flukes (Digenea, Fasciolidae). American Journal of Tropical Medicine and Hygiene 79, 248255.CrossRefGoogle Scholar
Mas-Coma, S, Valero, MA and Bargues, MD (2009) Chapter 2. Fasciola, lymnaeids and human fascioliasis, with a global overview on disease transmission, epidemiology, evolutionary genetics, molecular epidemiology and control. Advances in Parasitology 69, 41146.CrossRefGoogle ScholarPubMed
Mucheka, VT, Lamb, JM, Pfukenyi, DM and Mukaratirwa, S (2015) DNA sequence analyses reveal co-occurrence of novel haplotypes of Fasciola gigantica with F. hepatica in South Africa and Zimbabwe. Veterinary Parasitology 214, 144151.CrossRefGoogle ScholarPubMed
Nguyen, TGT, De, NV, Vercruysse, J, Dorny, P and Le, TH (2009) Genotypic characterization and species identification of Fasciola spp. with implications regarding the isolates infecting goats in Vietnam. Experimental Parasitology 123, 354361.CrossRefGoogle ScholarPubMed
Nolan, MJ and Cribb, TH (2005) The use and implications of ribosomal DNA sequencing for the discrimination of digenean species. Advances in Parasitology 60, 101163.CrossRefGoogle ScholarPubMed
Perera, BVP and Rajapakse, RPVJ (2009). Mortality and morbidity of wild elephants (Elephas maximus maximus) of Sri Lanka, as a result of liver flukes (Fasciola jacksoni) infestation. In Wibbelt, G, Kretschmar, P, Hofer, H and Seet, S (eds), Proceedings of the International Conference on Diseases of Zoo and Wild Animals. Leibniz Institute for Zoo and Wildlife Research, Beekse Bergen, The Netherlands, 20–24 May 2009, p. 191.Google Scholar
Perera, BVP, Rajapakse, RPVJ, Silva-fletcher, A, Thewarage, LD and Jinadasa, HRN (2015) Emerging parasitic and infectious diseases of wild Asian Elephants (Elephas Maximus) in Udawalawe National Park, Sri Lanka. American Association of Zoo Veterinarians. Oregon, USA.Google Scholar
Singh, KP, Srivastava, VK, Prasad, A and Pandey, AP (1994) Pathology due to Fasciola jacksoni in Indian elephants (Elephas indicus). Indian Journal of Animal Science 648, 802804.Google Scholar
Tkach, VV, Kudlai, O and Kostadinova, A (2016) Molecular phylogeny and systematics of the Echinostomatoidea Looss, 1899 (Platyhelminthes: Digenea). International Journal for Parasitology 46, 171185.CrossRefGoogle Scholar
Wey-Fabrizius, AR, Podsiadlowski, L, Herlyn, H and Hankeln, T (2013) Platyzoan mitochondrial genomes. Molecular Phylogenetics and Evolution 69, 365375.CrossRefGoogle ScholarPubMed
Windsor, R and Scott, W (1976) Fascioliasis and Salmonellosis in African elephants in captivity. British Veterinary Journal 132, 313317.CrossRefGoogle ScholarPubMed

Rajapakse et al. supplementary material

Table S1

File 19 KB

Rajapakse et al. supplementary material

Table S2

File 19 KB

Altmetric attention score

Full text views

Full text views reflects PDF downloads, PDFs sent to Google Drive, Dropbox and Kindle and HTML full text views.

Total number of HTML views: 31
Total number of PDF views: 132 *
View data table for this chart

* Views captured on Cambridge Core between 24th June 2019 - 11th April 2021. This data will be updated every 24 hours.

Send article to Kindle

To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Molecular characterization of Fasciola jacksoni from wild elephants (Elephas maximus maximus) of Sri Lanka: a taxonomic evaluation
Available formats
×

Send article to Dropbox

To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

Molecular characterization of Fasciola jacksoni from wild elephants (Elephas maximus maximus) of Sri Lanka: a taxonomic evaluation
Available formats
×

Send article to Google Drive

To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

Molecular characterization of Fasciola jacksoni from wild elephants (Elephas maximus maximus) of Sri Lanka: a taxonomic evaluation
Available formats
×
×

Reply to: Submit a response


Your details


Conflicting interests

Do you have any conflicting interests? *