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Protein and enery relationships in the broiler chicken 12. Dietary protein and triidothyronine (T3) effects on the response of broilers to isoproterenol and cyclic adenosine monophosphate in vitro*

Published online by Cambridge University Press:  09 March 2007

R.W. Rosebrough  and
A.D. Mitchell
R.W. Rosebrough
Affiliation:
Nonruminant Animal Nutrition Laboratory, Livestock and Poultry Science Institute, United States Department of Agriculture-Agricultural Research Service, Beltsville Agricultural Research Center, Beltsville, MD 20705, USA
A.D. Mitchell
Affiliation:
Nonruminant Animal Nutrition Laboratory, Livestock and Poultry Science Institute, United States Department of Agriculture-Agricultural Research Service, Beltsville Agricultural Research Center, Beltsville, MD 20705, USA
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Abstract

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Indian River male broiler chickens (7-d-old) were fed on diets containing 120, 210 or 300 g crude protein/kg + 0 or 1 mg triiodothyronine (T3)/kg diet (Expt 1) and 120, 150, 180 or 210 g crude protein/kg +0 or 1 mg T3/kg diet (Expt 2) to determine the effects of crude protein level and T3 on growth and metabolism. Body composition of chickens was determined by a combination of dissection of muscle and abdominal fat pads, and chemical extraction (Expt 1). In vitro lipogenesis (IVL) was determined in both experiments by incubating liver explants for 2 h at 37° in the presence of 10-4 M-dibutyryl cyclic AMP (cAMP) or 10-5 M isoproterenol (ISO) and 10-2 M-[2-14C]acetate. Acetate incorporation into total lipid was an indication of IVL. Activity ratios for each of these additions relative to control (?cAMP?ISO) were calculated to ascertain basal ν. inhibited rates of IVL. The relative muscle mass was increased by increasing crude protein from 120 to 210 g/kg diet but not from 210 to 300 g/kg diet. Dietary T3 decreased total body lipid regardless of the dietary crude-protein level. Increasing dietary crude protein decreased (P<0.05) basal IVL (?cAMP? ISO) but not IVL (+ cAMP). Dietary T3 decreased basal IVL in birds fed on the diets containing 120 and 210 g crude protein/kg but had little effect on the two inhibited states of lipogenesis (+ cAMP or +ISO). The component of lipogenesis sensitive to in vitro inhibition is also the component under dietary control.

Keywords

Dietary proteinTriiodothyronineLipogenesisChicken
Type
Chickhen liver metabolism in vitro
Information
British Journal of Nutrition , Volume 73 , Issue 1 , January 1995 , pp. 73 - 85
Copyright
Copyright © The Nutrition Society 1995

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