Book contents
- A Textbook of Clinical Embryology
- A Textbook of Clinical Embryology
- Copyright page
- Contents
- Frontispiece
- Chapter Co-authors
- Acknowledgments
- Section 1 Physiology of Reproduction
- Section 2 Assisted Reproductive Procedures
- Chapter 6 Hormonal Ovarian Treatment
- Chapter 7 Luteal Support
- Chapter 8 Ovum Pickup (OPU)
- Chapter 9 In Vitro Fertilization (IVF)
- Chapter 10 Embryo Culture
- Chapter 11 Embryo Transfer
- Chapter 12 Cryopreservation
- Chapter 13 In Vitro Maturation (IVM)
- Chapter 14 Biopsy of Testicles
- Chapter 15 Donation and Surrogacy
- Section 3 Genetics and Preimplantation Genetic Testing
- Section 4 IVF Laboratory
- Index
- References
Chapter 10 - Embryo Culture
from Section 2 - Assisted Reproductive Procedures
Published online by Cambridge University Press: 05 March 2021
- A Textbook of Clinical Embryology
- A Textbook of Clinical Embryology
- Copyright page
- Contents
- Frontispiece
- Chapter Co-authors
- Acknowledgments
- Section 1 Physiology of Reproduction
- Section 2 Assisted Reproductive Procedures
- Chapter 6 Hormonal Ovarian Treatment
- Chapter 7 Luteal Support
- Chapter 8 Ovum Pickup (OPU)
- Chapter 9 In Vitro Fertilization (IVF)
- Chapter 10 Embryo Culture
- Chapter 11 Embryo Transfer
- Chapter 12 Cryopreservation
- Chapter 13 In Vitro Maturation (IVM)
- Chapter 14 Biopsy of Testicles
- Chapter 15 Donation and Surrogacy
- Section 3 Genetics and Preimplantation Genetic Testing
- Section 4 IVF Laboratory
- Index
- References
Summary
Global tissue culture media were first developed by Ludwig and Ringer, nearly 150 years ago. These were simple salt solutions, simulating the properties of serum. The second generation of culture media, developed in the 1970s, mimics the female reproductive tract environment. A new, third generation of media was more improved and provided optimal growth conditions in vitro. The shift from home-brewed culture media to commercially manufactured media has contributed to improvements in laboratory and clinical outcomes, eliminating inconsistencies, manufacturing errors, and batch-to-batch variability.
- Type
- Chapter
- Information
- A Textbook of Clinical Embryology , pp. 114 - 125Publisher: Cambridge University PressPrint publication year: 2021