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Metaphase arrest in newly matured or microtubule-depleted mouse eggs after calcium stimulation

Published online by Cambridge University Press:  26 September 2008

Ruth M. Moses  and
Yoshio Masui
Ruth M. Moses*
Affiliation:
Department of Zoology, University of Toronto, Toronto, Canada
Yoshio Masui
Affiliation:
Department of Zoology, University of Toronto, Toronto, Canada
*
Dr Ruth Moses, Department of Zoology, University of Toronto, 25, Harbord Street, Toronto, Ontario, Canada M5S 1A1. Telephone: (416) 978-3493. Fax: (416)978-8532.
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Summary

In mouse eggs arrested at meiotic metaphase II, the increase in intracellular calcium that results from fertilisation induces nuclear formation in both newly ovulated and older eggs. In contrast, the calcium increase that results from exposure to the calcium ionophore A23187 induces nuclear formation in older, but not young, newly ovulated eggs. When treated with the microtubule inhibitor colcemid, and fertilised, young eggs remained at metaphase, but many older eggs formed nuclei, although older eggs treated with colcemid and A23187 remained at metaphase. However, young A23187-treated eggs, young colcemid-treated fertilised eggs, and older colcemid- and A23187-treated eggs, formed nuclei when treated, in addition, with the protein synthesis inhibitor cycloheximide, or the protein kinase inhibitor 6-dimethylaminopurine (6-DMAP). The possibility is discussed that metaphase in newly matured eggs and microtubule-depleted eggs may be maintained by similar mechanisms involving short-lived phosphorylated proteins.

Keywords

6-DimethylaminopurineMetaphase arrestMicrotubule inhibitorsNewly matured mouse eggsProtein synthesis inhibitors
Type
Article
Information
Zygote , Volume 3 , Issue 1 , February 1995 , pp. 1 - 8
Copyright
Copyright © Cambridge University Press 1995

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