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The response of mouse oocytes injected with sea urchin spermatozoa

Published online by Cambridge University Press:  26 September 2008

T. Wakayama ,
T. Uehara ,
Y. Hayashi  and
R. Yanagimachi
T. Wakayama
Affiliation:
University of Hawaii School of Medicine, University of Tokyo and Ryukyn University
T. Uehara
Affiliation:
University of Hawaii School of Medicine, University of Tokyo and Ryukyn University
Y. Hayashi
Affiliation:
University of Hawaii School of Medicine, University of Tokyo and Ryukyn University
R. Yanagimachi*
Affiliation:
University of Hawaii School of Medicine, University of Tokyo and Ryukyn University
*
Ryuzo Yanagimachi, Department of Anatomy and Reproductive Biology, University of Hawaii School of Medicine, Honolulu, HI 96822, USA. Tel: 808-956-8746. Fax: 808-956-5474. e-mail: yana@hawaii.edu.
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Summary

Sea urchin spermatozoa were injected into mature mouse oocytes to determine whether they can activate mouse oocytes and, if so, how they behave within the oocyte cytoplasm of such a distant species. While injection of a single spermatozoon into each oocyte did not activate any of the oocytes, injection of 10 spermatozoa activated about 20%. Within the cytoplasm of unactivated oocytes, sperm heads commonly transformed into chromosome-like structures. When a single spermatozoon was injected, and oocytes were then activated by Sr2+, about 30% of the activated oocytes had both female (mouse) and male (sea urchin) pronuclei when examined 8 h after sperm injection. These results indicated that sperm-borne oocyte activating factor(s) and the cytoplasmic factors controlling the development of the sperm pronucleus are not strictly species-specific.

Keywords

ActivationMouseOocyteSea urchinSperm
Type
Article
Information
Zygote , Volume 5 , Issue 3 , August 1997 , pp. 229 - 234
Copyright
Copyright © Cambridge University Press 1997

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