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Inhibition of Photochemical Activity of Isolated Chloroplasts by Acylanilides

Published online by Cambridge University Press:  12 June 2017

D. E. Moreland  and
K. L. Hill
D. E. Moreland
Affiliation:
Crops Research Division, Agricultural Research Service, U. S. Department of Agriculture, Field Crops Department, N. C. State College, Raleigh, N. C.
K. L. Hill
Affiliation:
Crops Research Division, Agricultural Research Service, U. S. Department of Agriculture, Field Crops Department, N. C. State College, Raleigh, N. C.
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Abstract

The effect of mono-and dichlorophenyl analogs of N-phenyl-2-methylpentanamide (PMP) and N-(3,4-dichlorophenyl)-alkylamides on the photolytic activity (Hill reaction) of chloroplasts isolated from turnip greens (Brassica spp.) was studied. Photochemical activity was measured potentiometrically with ferricyanide as the electron acceptor. PMP inhibited the Hill reaction by 50 percent (I50) at 4.5 × 10-5 M. Monochlorination in the meta and para ring positions enhanced the inhibitory activity whereas chlorination in the ortho position negated inhibitory activity of the parent chemical (PMP). N-(3,4-dichlorophenyl)-2-methylpentanamide (3,4-DCPMP) was the most inhibitory of the 6 dichlorinated isomers (I50 = 5.8 × 10-7 M). Replacement of the imino hydrogen of 3,4-DCPMP with a methyl group reduced inhibitory activity below that of the unsubstituted parent compound (PMP). Of the straight-chain N-(3,4-dichlorophenyl)alkylamides studied, the propanamide expressed maximum inhibition. 2-Methylalkylamides were more inhibitory than the corresponding straight-chain alkylamides. Maximum inhibition in the branched series was obtained with 3,4-DCPMP. Kinetic studies performed with 3,4-DCPMP at different light intensities indicated that both light and dark reactions were inhibited. However, the light reaction was more sensitive to the effects of the chemical.

Type
Research Article
Information
Weeds , Volume 11 , Issue 1 , January 1963 , pp. 55 - 60
Copyright
Copyright © 1963 Weed Science Society of America 

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References

Literature Cited

1. Bayliss, N. S. 1951. The migration of excitation energy. Rev. Pure and Appl. Chem. 1:67–76.Google Scholar
2. Good, N. E. 1961. Inhibitors of the Hill reaction. Plant Physiol. 36:788–803.Google Scholar
3. Krogmann, D. W., and Jagendorf, A. T. 1959. Inhibition of the Hill reaction by fatty acids and metal chelating agents. Arch. Biochem. and Biophys. 80:421–430.Google Scholar
4. Lumry, R., Spikes, J. D., and Eyring, H. 1954. Photosynthesis. Ann. Rev. Plant Physiol. 5:271–340.Google Scholar
5. Moreland, D. E., and Hill, K. L. 1959. The action of alkyl N-phenylcarbamates on the photolytic activity of isolated chloroplasts. J. Agr. and Food Chem. 7:832–837.Google Scholar
6. Moreland, D. E., and Hill, K. L. 1960. Inhibition of the photochemical activity of isolated chloroplasts by ring-chlorinated N-phenyl-2-methylpentanamides. Abstracts, WSA, 41–42.Google Scholar
7. Moreland, D. E., and Hill, K. L. 1962. Interference of herbicides with the Hill reaction of isolated chloroplasts. Weeds 10:229–236.Google Scholar
8. Spikes, J. D. 1956. Effects of substituted ureas on the photochemical activity of isolated chloroplasts. Plant Physiol. Suppl. 31:xxxii.Google Scholar
9. Wessels, J. S. C. 1954. A possible function of vitamin K in photosynthesis. Rec. trav. chim. 73:529–536.CrossRefGoogle Scholar
10. Wirtz, K. 1950. Leitungseffekte in H-Brückensystemen. Z. Elecktrochem. 54:47–51.Google Scholar