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Enzymatic Hydrolysis of Herbicides in Plants

Published online by Cambridge University Press:  12 June 2017

R. E. Hoagland
Affiliation:
Dep. of Biochem., N. Dak. State Univ., Fargo, North Dakota 58102
G. Graf
Affiliation:
Dep. of Biochem., N. Dak. State Univ., Fargo, North Dakota 58102

Abstract

Nineteen different genera of weeds encompassing twelve plant families were surveyed for hydrolytic enzymes which were able to metabolize the amide bonds in several groups of herbicides. Propanil (3′,4′-dichloropropionanilide), 1,1-dimethyl-3-phenylurea (fenuron), and isopropyl carbanilate (propham) were employed as enzyme substrates, representing the amide, urea, and carbamate classes. Propanil was hydrolyzed at widely diverse rates by approximately 70% of the plants tested. Only one plant showed a hydrolysis of propham and fenuron. The study demonstrates a widespread distribution of hydrolytic enzymes capable of degrading the herbicide, propanil. An apparent lack of hydrolytic enzymes able to metabolize propham and fenuron was also noted.

Type
Research Article
Copyright
Copyright © Weed Science Society of America 

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References

Literature Cited

1. Audus, L. J. 1964. The classification of herbicides and types of toxicity, p. 137. In Audus, L. J. (Ed) The physiology and biochemistry of herbicides. Academic Press, London and New York.Google Scholar
2. Borner, H. 1965. Untersuchungen über den Abbau von Afalon (N-3,4-Dichlorophenyl)-N′-methylharnstoff) and Aresin (N-4-Chlorphenyl)-N′-methoxy-N-methylharnstoff) im Boden. Z. Pflanzenkr. Pflanzenpathol. Pflanzenschutz 72: 516531.Google Scholar
3. Dalton, R. L., Evans, A. W., and Rhodes, R. C. 1966. Disappearance of diuron from cotton field soils. Weeds 14:3133.CrossRefGoogle Scholar
4. Frear, D.S. 1968. Microsomal N-demethylation, by a cotton leaf oxidase system, of 3-(4′-chlorophenyl)-1,1-dimethylurea (monuron). Science 162:674675.Google Scholar
5. Frear, D. S. and Still, G. G. 1968. The metabolism of 3,4-dichloropropionanilide in plants. Partial purification and properties of an aryl acylamidase from rice. Phytochemistry 7:913920.Google Scholar
6. Geissbühler, H., Haselbach, C., Aebi, H., and Ebner, L. 1963. The fate of N′-(4-chlorophenoxy)-phenyl-N,N-dimethylurea in soils and plants. Weed Res. 3:277297.Google Scholar
7. Hoagland, R. E. and Graf, G. 1972. An aryl acylamidase from tulip which hydrolyzes 3′,4′-dichloropropionanilide. Phytochemistry 11:521527.Google Scholar
8. James, C. S. and Prendeville, G. N. 1969. Metabolism of chlorpropham (isopropyl m-carbanilate) in various plant species. J. Agr. Food Chem. 7:12571260.Google Scholar
9. Jaworski, E. G. 1967. Symposium on decomposition and metabolism of herbicides. J. Agr. Food Chem. 15:557604.Google Scholar
10. Kearney, P. C. 1965. Purification and properties of an enzyme responsible for hydrolyzing phenylcarbamates. J. Agr. Food Chem. 13:561564.Google Scholar
11. Kearney, P. C. and Kaufman, D. D. 1965. Enzyme from soil bacterium which hydrolyzes phenylcarbamate herbicides. Science 147:740741.Google Scholar
12. Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265275.Google Scholar
13. Menzie, C. M. 1969. Metabolism of pesticides. U. S. Dep. of Interior, Special Scientific Report, Wildlife No. 96, Washington, D. C. 487 p.Google Scholar
14. Onley, H. J., Yip, G., and Aldridge, M. H. 1968. A metabolic study of 3-(3,4-dichlorophenyl)-1,1-dimethylurea (diuron) applied to corn seedlings. J. Agr. Food Chem. 16: 426433.Google Scholar
15. Riden, J. R. and Hopkins, T. R. 1961. Decline and residue studies on 4-chloro-2-butynyl N-(3-chlorophenyl) carbamate. J. Agr. Food Chem. 9:4749.Google Scholar
16. Riden, J. R. and Hopkins, T. R. 1962. Formation of a water-soluble 3-chloroaniline-containing substance in barban treated plants. J. Agr. Food Chem. 10:455458.Google Scholar
17. Smith, J. W. and Sheets, T. J. 1967. Uptake, distribution and metabolism of monuron and diuron by several plants. J. Agr. Food Chem. 15:577581.CrossRefGoogle Scholar
18. Still, G. G. and Mansager, E. R. 1971. Metabolism of isopropyl 3-chlorocarbanilate by soybean plants. J. Agr. Food Chem. 19:879884.Google Scholar
19. Swanson, C. R. and Swanson, H. R. 1968. Metabolic fate of monuron and diuron in isolated leaf discs. Weed Sci. 16:137143.Google Scholar