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Cloning and Characterization of a Critical Meristem Developmental Gene (EeSTM) from Leafy Spurge (Euphorbia esula)

Published online by Cambridge University Press:  20 January 2017

Vijaya Varanasi ,
Tracey Slotta  and
David Horvath
Vijaya Varanasi
Affiliation:
Department of Plant Sciences, North Dakota State University, State University Station, Fargo ND 58105
Tracey Slotta
Affiliation:
U.S. Department of Agriculture, Agricultural Research Service, Biosciences Research Laboratory, P.O. Box 5674, State University Station, Fargo, ND 58105-5674
David Horvath*
Affiliation:
Department of Plant Sciences, North Dakota State University, Fargo, ND 58105
*
Corresponding author's E-mail: horvathd@fargo.ars.usda.gov
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Abstract

SHOOTMERISTEMLESS (STM) encodes a member of the class I KNOX homeodomain protein family that is required for meristem development and maintenance. We have isolated both genomic and cDNA clones of STM from the perennial weed leafy spurge. A comparison to other class I KNOX genes indicates that EeSTM represents an orthologue of AtSTM and not one of the other class I KNOX gene family members. 5′ rapid amplification of cDNA ends (RACE) indicated that the transcription initiation site is close to the start of translation and is conserved between arabidopsis and leafy spurge. Putative cis-acting elements were identified in the EeSTM promoter, including a tuber-specific sucrose-responsive element, which could play a major role in the expression of EeSTM in root tissue.

Keywords

Arabidopsis, Arabidopsis thaliana L. ARATHleafy spurge, Euphorbia esula L. EPHESSHOOTMERISTEMLESSmeristem developmentpromoter analysisphylogenetics
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 56 , Issue 4 , August 2008 , pp. 490 - 495
Copyright
Copyright © Weed Science Society of America 

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