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Relationship among Lepista species determined by CAPS and RAPD

Published online by Cambridge University Press:  28 February 2005

Karen STOTT
Affiliation:
Unité de Recherches sur les Champignons, Institut National de la Recherche Agronomique, BP 81, 33883 Villenave d'Ornon, Cedex, France. E-mail: karen.stott@dpiwe.tas.gov.au; karen.stott@utas.edu.au Present address: Tasmanian Institute of Agricultural Research, New Town Research Laboratories, 13 St Johns Avenue, New Town, TAS 7008, Australia.
Christophe DESMERGER
Affiliation:
Unité de Recherches sur les Champignons, Institut National de la Recherche Agronomique, BP 81, 33883 Villenave d'Ornon, Cedex, France. E-mail: karen.stott@dpiwe.tas.gov.au; karen.stott@utas.edu.au Centre Technique du Champignon, 22 rue Bizard, Munet, 49400 Distré, France.
Paul HOLFORD
Affiliation:
Centre for Horticulture and Plant Sciences, University of Western Sydney, Locked Bag 1797, Penrith South DC, NSW 1797, Australia.
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Abstract

To determine the relationship of Australian members of the genus Lepista with those from other parts of the world, genetic variation of isolates representing 27 accessions was assayed by cleaved amplified polymorphic sequences (CAPS) and random amplified polymorphic DNA (RAPD). CAPS and RAPD identified eight and seven groups, respectively. CAPS Groups 1 and 2 and RAPD Group 1 consisted of French and Australian accessions classified as morphospecies L. nuda. CAPS Group 3 and RAPD Groups 2 and 2A consisted of mostly Australian isolates identified as L. sordida var. sordida or L. sordida var. umbonata. Isolates earlier identified as morphospecies L. sp. were also placed in CAPS Group 3 and RAPD Group 2A indicating that these isolates are L. sordida var. sordida. In addition, three smaller groups were distinguished. A French isolate of L. sordida var. sordida was placed in distinctly separate CAPS and RAPD groups to Australian L. sordida var. sordida Groups 4,4 respectively. A French isolate of L. sordida var. aianthina was placed in CAPS and RAPD Groups 3,3. An accession of L. saeva was placed in CAPS Group 6 and RAPD Group 5, separate from other isolates. RAPD Groups 6 and 7 consist, respectively, of Greek and American accessions of L. nuda that were only distantly related to the Australian and French accessions of this morphospecies: CAPS also separated these isolates from each other and from all other isolates.

The data suggest that the classification of morphospecies and varieties within Lepista cannot be determined on the basis of morphology alone. The Greek and American accessions of L. nuda are separated from the French and Australian accessions and may not be L. nuda. Similarly, the Australian accessions currently classified as L. sordida var. sordida together with the accessions of L. sordida var. umbonata are distinct from the French accessions of L. sordida var. sordida suggesting that Australian isolates may represent a new species or variety. Further studies using a combination of morphological and genetic characterization are required to provide a more accurate understanding of Lepista species.

Type
Research Article
Copyright
© The British Mycological Society 2005

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