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Toxicity Effect of Cancer Cell Labeled with Visible Luminescent Nanocrystalline Silicon Particles and Visualization Observation in Vivo

Published online by Cambridge University Press:  01 February 2011

Keisuke Sato ,
Masaki Hiruoka ,
Kohki Fujioka ,
Naoki Fukata ,
Kenji Hirakuri  and
Kenji Yamamoto
Keisuke Sato
Affiliation:
SATO.Keisuke@nims.go.jp, National Institute for Materials Scinece (NIMS), World Premier International (WPI) Research Center, International Center for Materials Nanoarchitectonics (MANA), Tsukuba, Japan
Masaki Hiruoka
Affiliation:
mhiruoka@ff.f.dendai.ac.jp, Tokyo Denki University, Department of Electronic and Computer Engineering, Hikigun, Saitama, Japan
Kohki Fujioka
Affiliation:
kfujioka@ri.imcj.go.jp, International Medical Center of Japan, Department of Medical Ecology and Informatics, Shinjuku, Tokyo, Japan
Naoki Fukata
Affiliation:
FUKATA.Naoki@nims.go.jp, National Institute for Materials Scinece (NIMS), World Premier International (WPI) Research Center, International Center for Materials Nanoarchitectonics (MANA), Tsukuba, Ibaraki, Japan
Kenji Hirakuri
Affiliation:
hirakuri@eee.dendai.ac.jp, Tokyo Denki University, Department of Electronic and Computer Engineering, Hikigun, Saitama, Japan
Kenji Yamamoto
Affiliation:
backen@tcn-catv.ne.jp, International Medical Center of Japan, Department of Medical Ecology and Informatics, Shinjuku, Tokyo, Japan
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Abstract

Cytotoxicity of human cervical carcinoma cell line (HeLa cells) labeled with the nanocrystalline silicon (nc-Si) particles before and after ultraviolet (UV) light exposure has studied on the viability and cellular membrane damages. The viability and cellular membrane damages of HeLa cells changed at high particle concentration of 1.12 mg/ml. The viability of HeLa cells labeled with the UV-exposed nc-Si particles was higher than that of unexposed nc-Si particles. However, the variation of cellular membrane damages was almost same for the nc-Si particles before and after UV exposure. These results substantiated the low toxicity of nc-Si particles. Moreover, the HeLa cells labeled with the nc-Si particles exhibited green fluorescence. On the other hand, in vivo test of nc-Si particles estimated by the visualization observation of the circulation from the lymphatic vessel to the lymph node of a mouse. The transfer pathway of nc-Si particles could be clearly monitored by the strong emission of red light.

Keywords

biomaterialluminescencenanostructure
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1145: Symposium MM – Application of Group IV Semiconductor Nanostructures , 2008 , 1145-MM04-23
Copyright
Copyright © Materials Research Society 2009

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References

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