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Nanoscale Measurements of Water Loss during Desiccation of Biological Cell Suspensions

Published online by Cambridge University Press:  01 February 2011

S. Mittal  and
R.V. Devireddy
S. Mittal
Affiliation:
Bioengineering Laboratory, Department of Mechanical Engineering, Louisiana State University, Baton Rouge, LA 70803, U.S.A.
R.V. Devireddy
Affiliation:
Bioengineering Laboratory, Department of Mechanical Engineering, Louisiana State University, Baton Rouge, LA 70803, U.S.A.
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Abstract

There is an urgent need to protect and conserve the endangered species of the world. Desiccation, or the phenomenon of anhydrobiosis, offers the attractive possibility of low cost, long term storage of reproductive tissues and cells from endangered species at ambient temperatures. Biophysical optimization of anhydrobiosis procedures requires dynamic and accurate quantification of the rate of moisture loss during a prescribed desiccation protocol. Engineered microstructures can serve as a multifunctional and highly sensitive method to measure the rate of moisture loss. This study presents a novel ultrasound based (resonant) mass sensor to quantify water loss during a drying process by measuring the corresponding shift in the resonant frequency of a micromachined vibrating structure. To model the proposed ultrasound based sensor, we performed a static (stress) and dynamic (frequency change with change in imposed mass) analysis. The results suggest that by tailoring the dimension of the vibrating element appropriate sensitivity can be achieved.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 844: Symposium Y – Mechanical Properties of Bio-Inspired and Biological Materials , 2004 , Y6.11
Copyright
Copyright © Materials Research Society 2005

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References

REFERENCES

1. “Life in frozen State” Edited by B.J. Fuller, N. Lane and E.E. Benson. CRC Press, Boca Raton, FL (2004).Google Scholar
2. Bhowmick, S., Zhu, L., McGinnis, L., Lawitts, J., Nath, B.D., Toner, M., and Biggers, J., “Desiccation Tolerance of Spermatozoa Dried at Ambient Temperature: Production of Fetal Mice” Biology of Reproduction 68, 1779–1786 (2003).Google Scholar
3. Schomburg, W.K., Vitt, M., Bacher, W., Borner, M.W. and Menz, W., “Measurements of Physical parameters with Ultrasound and Microdiaphragms” IEEE Proceedings of Micro Electromechanical Systems 1995 (MEMS'95), pg. 139.Google Scholar
4. “Formulas for Natural Frequency and Mode Shape” R.D. Blevins, Krieger Publishing Co, Melbourne, FL (2001).Google Scholar
5. Boay, C.G., “Frequency Analysis of Rectangular Isotropic Plates Carrying a Concentrated Mass”, Computers and Structures 56 (1) 39–48 (1995).Google Scholar
6. Ostachowicz, W., Krawczuk, M., Cartmell, M., “The location of a concentrated mass on rectangular plates from measurements of natural vibration”, Computers and Structures 80 1419–1428 (2002).Google Scholar