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Biologically Engineered Quantum Dots for Biomedical Applications

  • Ganna Chornokur (a1), Sergei Ostapenko (a2), Yusuf Emirov (a3), Nadezhda Korsunska (a4), Abraham Wolcott (a5), Jin Zhang (a6), Catherine Phelan (a7), Abhilasha Nagaram (a8) and Thomas Sellers (a9)...

Abstract

We report on a short-wavelength, “blue” spectral shift of the photoluminescence (PL) spectrum in CdSeTe/ZnS core/shell quantum dots (QDs) caused by bioconjugation with several monoclonal cancer related antibodies (ABs). Scanning PL spectroscopy was performed on samples dried on solid substrates at various temperatures. The influence of the AB chemical origin on the PL spectral shift was observed. The conjugation QD-AB reaction was confirmed using the agarose gel electrophoresis technique. The spectral shift is strongly increased and the process facilitated when the samples are dried above room temperature. The PL spectroscopic mapping revealed a profile of the PL spectral shift across the dried QD-AB spot. Transmission Electron Microscopy analyses of the samples were performed to reveal the shape and size of individual QDs. A mechanism of the “blue” shift is attributed to changes in the QD electronic energy levels caused by local stress field applied to the bio-conjugated QD.

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1. National Vital Statistics Report 2007 55 (19)
2.Human PSA ELISA Kit from Abazyme: http://www.biocompare.com/itemdetails.asp?itemid=434262
3. Dybiec, M, Chornokur, G, Ostapenko, S, Wolcott, A, JZ, Zhang, Zajac, A, Phelan, C and Sellers, T 2007 Photoluminescence spectroscopy of bioconjugated CdSe/ZnS quantum Dots Appl. Phys Lett 90 263112
4.Official Invitrogen website: http://www.invitrogen.com
5. Udenfriend, S, Stein, S, Böhlen, P, Dairman, W, Leimgruber, W and Weigele, M 1972 Fluorescamine: a reagent for assay of amino acids, peptides, proteins, and primary amines in the picomole range Science, New Series 178(4063) 871–72.
6. AM, Skelley and RA, Mathies 2003 Chiral separation of fluorescamine-labeled amino acids using microfabricated capillary electrophoresis devices for extraterrestrial exploration J Chromatography A 1021 191–99
7. Shahzad, K, DJ, Olego and CJ, Van de Walle 1988 Optical characterization and band offsets in ZnSe-ZnSxSe1-x strained-layer superlattices Phys. Rev. B 38 1417
8. Borkovska, L, Korsunska, N, Krystab, T, Pecherska, E, Germash, L, Ostapenko, S and Chornokur, G In press The influence of bioconjugation on photoluminescence and structural characteristics of quantum dots CdSe/ZnS Semiconductors
9. SA, Empedocles and MG, Bawendi 1997 Science 278 2114–39
10. Oda, M, Tsukamoto, J, Hasegawa, A, Iwami, N, Nishiura, K, Hagiwara, I, Ando, N, Horiuchi, H and Tani, T 2006 Photoluminescence of CdSe/ZnS/TOPO nanocrystals expanded on silica glass substrates: adsorption and desorption effects of polar molecules on nanocrystal surfaces J.Lumin. 570 110–20

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