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Amorphous Silicon Photosensors for Detection of Intrinsic Cell Fluorophores

Published online by Cambridge University Press:  03 August 2011

A. Joskowiak ,
V. Chu ,
D.M.F. Prazeres  and
J.P. Conde
A. Joskowiak
Affiliation:
INESC Microsistemas e Nanotecnologias and IN-Institute of Nanoscience and Nanotechnology, Rua Alves Redol 9, Lisbon, Portugal. Department of Bioengineering, Instituto Superior Tecnico, Av. Rovisco Pais, Lisbon, Portugal IBB – Institute for Biotechnology and Bioengineering, Instituto Superior Tecnico, Av. Rovisco Pais, Lisbon, Portugal
V. Chu
Affiliation:
INESC Microsistemas e Nanotecnologias and IN-Institute of Nanoscience and Nanotechnology, Rua Alves Redol 9, Lisbon, Portugal.
D.M.F. Prazeres
Affiliation:
Department of Bioengineering, Instituto Superior Tecnico, Av. Rovisco Pais, Lisbon, Portugal IBB – Institute for Biotechnology and Bioengineering, Instituto Superior Tecnico, Av. Rovisco Pais, Lisbon, Portugal
J.P. Conde
Affiliation:
INESC Microsistemas e Nanotecnologias and IN-Institute of Nanoscience and Nanotechnology, Rua Alves Redol 9, Lisbon, Portugal. Department of Bioengineering, Instituto Superior Tecnico, Av. Rovisco Pais, Lisbon, Portugal
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Abstract

An amorphous silicon (a-Si:H) photoconductor array with two distinct integrated amorphous silicon carbon alloy (a-SiC:H) high pass filters is used to detect two of the cell intrinsic fluorophores. The cutoff wavelength of the filters is tuned by the carbon content in the film. The fluorophores of interest – reduced nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD) are indicative of the redox state of the cells. Concentrations down to 1 μM for NADH and 50 μM for FAD were detected.

Keywords

amorphousSisensor
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1321: Symposium A – Amorphous and Polycrystalline Thin-Film Silicon Science and Technology , 2011 , mrss11-1321-a20-01
Copyright
Copyright © Materials Research Society 2011

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References

REFERENCES

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