Hostname: page-component-78c5997874-dh8gc Total loading time: 0 Render date: 2024-11-17T16:32:42.888Z Has data issue: false hasContentIssue false
  • English
  • Français

Mineralogical characterization of calcification in cardiovascular aortic atherosclerotic plaque: A case study

Published online by Cambridge University Press:  05 July 2018

Yan Li ,
Xin Wang ,
Meiqian Zhu ,
Chong-Qing Yang ,
Anhuai Lu ,
Kang Li ,
Fanlu Meng  and
Changqiu Wang
Yan Li*
Affiliation:
The Key Laboratory of Orogenic Belts and Crustal Evolution, School of Earth and Space Sciences, Peking University, Beijing, P.R. China
Xin Wang*
Affiliation:
The Key Laboratory of Orogenic Belts and Crustal Evolution, School of Earth and Space Sciences, Peking University, Beijing, P.R. China
Meiqian Zhu
Affiliation:
The Key Laboratory of Orogenic Belts and Crustal Evolution, School of Earth and Space Sciences, Peking University, Beijing, P.R. China
Chong-Qing Yang
Affiliation:
Department of Pathology, Beijing Hospital, Beijing, P.R. China
Anhuai Lu*
Affiliation:
The Key Laboratory of Orogenic Belts and Crustal Evolution, School of Earth and Space Sciences, Peking University, Beijing, P.R. China
Kang Li
Affiliation:
Department of Cardiology, Beijing Hospital, Beijing, P.R. China
Fanlu Meng
Affiliation:
The Key Laboratory of Orogenic Belts and Crustal Evolution, School of Earth and Space Sciences, Peking University, Beijing, P.R. China
Changqiu Wang
Affiliation:
The Key Laboratory of Orogenic Belts and Crustal Evolution, School of Earth and Space Sciences, Peking University, Beijing, P.R. China
*
§ The first two authors contributed equally to this work.
§ The first two authors contributed equally to this work.
* E-mail:ahlu@pku.edu.cn
Get access Rights & Permissions [Opens in a new window]

Abstract

Calcification in cardiovascular aortic atherosclerotic plaque contains Ca-phosphate minerals. However, most research on cardiovascular calcification has focused on its physiological properties rather than its mineralogical features. In this present study, cardiovascular calcification was characterized by collecting samples from patients’ tissues and applying mineralogical techniques. Synchrotron radiation-based micro-X-ray diffraction showed the calcification had a similar structure to hydroxylapatite (HAp). Transmission electron microscopy showed some structurally HAp-like spherical particles with a diameter of ∼200 nm and acicular crystals ∼100 nm × ∼20 nm in size. Selected-area electron diffraction indicated that these mineral particles belonged to the hexagonal crystal system. Fourier-transform infrared (FTIR) spectroscopy showed three typical peaks at 1469 cm−1, 1455 cm−1 and 1413 cm−1, indicating that the carbonate group in the calcification plaque substituted for a hydroxyl group to form B-type CHAp (Ca10(PO4,CO3)x (OH)y ). The FTIR mapping results illustrated the intergrowth of calcification and organic tissues and the inhomogeneous substitution of phosphate by carbonate in the calcification area. X-ray absorption near-edge structure analysis affirmed that the chemical environments of Ca in the calcification were close to those in HAp. Based on these mineralogical characteristics, the calcification in plaque is identified as a mixture phase of HAp and B-type carbonate HAp, which is similar to the composition of bones.

Keywords

Pathological mineralizationcalcificationcardiovascular diseasecarbonated hydroxylapatite
Type
Research Article
Information
Mineralogical Magazine , Volume 78 , Issue 4 , August 2014 , pp. 775 - 786
Copyright
Copyright © The Mineralogical Society of Great Britain and Ireland 2014

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Abedin, M., Tintut, Y. and Demer, L.L. (2004) Vascular calcification – mechanisms and clinical ramifications. Arteriosclerosis Thrombosis and Vascular Biology, 24, 1161–1170.CrossRefGoogle ScholarPubMed
Avwioro, G. (2011) Histochemical uses of haematoxylin – a review. Journal of Pharmacy and Clinical Sciences, 1, 24–34.Google Scholar
Barinov, S., Rau, J., Cesaro, S., Ďurišin, J., Fadeeva, I., Ferro, D., Medvecky, L. and Trionfetti, G. (2006) Carbonate release from carbonated hydroxyapatite in the wide temperature rage. Journal of Materials Science: Materials in Medicine, 17, 597–604.Google ScholarPubMed
Barralet, J., Best, S. and Bonfield, W. (1998) Carbonate substitution in precipitated hydroxyapatite: An investigation into the effects of reaction temperature and bicarbonate ion concentration. Journal of Biomedical Materials Research, 41, 79–86.3.0.CO;2-C>CrossRefGoogle ScholarPubMed
Bild, D.E., Detrano, R., Peterson, D., Guerci, A., Liu, K., Shahar, E., Ouyang, P., Jackson, S. and Saad, M.F. (2005) Ethnic differences in coronary calcification – the multiethnic study of atherosclerosis (mesa). Circulation, 111, 1313–1320.CrossRefGoogle Scholar
Bobryshev, Y.V. (2005) Calcification of elastic fibers in human atherosclerotic plaque. Atherosclerosis, 180, 293–303.CrossRefGoogle ScholarPubMed
Boström, K. (2005) Proinflammatory vascular calcification. Circulation Research, 96, 1219–1220.CrossRefGoogle ScholarPubMed
Boström, K., Watson, K.E., Horn, S., Wortham, C., Herman, I.M. and Demer, L.L. (1993). Bone morphogenetic protein expression in human atherosclerotic lesions. Journal of Clinical Investigation, 91(4), 1800–1809.CrossRefGoogle ScholarPubMed
Brown, W.E., Eidelman, N. and Tomazic, B. (1987) Octacalcium phosphate as a precursor in biomineral formation. Advances in Dental Research, 1, 306–313.CrossRefGoogle ScholarPubMed
Camacho, N.P., West, P., Torzilli, P.A. and Mendelsohn, R. (2001) FTIR microscopic imaging of collagen and proteoglycan in bovine cartilage. Biopolymers, 62, 1–8.3.0.CO;2-O>CrossRefGoogle ScholarPubMed
Carpentier, X., Bazin, D., Jungers, P., Reguer, S., Thiaudiere, D. and Daudon, M. (2010) The pathogenesis of Randall’s plaque: a papilla cartography of Ca compounds through an ex vivo investigation based on XANES spectroscopy. Synchrotron Radiation, 17, 374–379.CrossRefGoogle Scholar
Chaboy, J. and Quartieri, S. (1995) X-ray absorption at the Ca K-edge in natural-garnet solid solutions: A full-multiple-scattering investigation. Physical Review B, 52, 6349–6357.CrossRefGoogle ScholarPubMed
Dekker, R.J., de Bruijn, J.D., Stigter, M., Barrere, F., Layrolle, P. and van Blitterswijk, C.A. (2005) Bone tissue engineering on amorphous carbonated apatite and crystalline octacalcium phosphate-coated titanium discs. Biomaterials, 26, 5231–5239.CrossRefGoogle ScholarPubMed
Dhore, C.R., Cleutjens, J.P.M., Lutgens, E., Cleutjens, K.B.J.M., Geusens, P.P.M., Kistslaar, P.J.E.H.M., Tordoir, J.H.M., Spronk, H.M.H., Vermeer, C. and Daemen, M.J.A.P. (2001) Differential expression of bone matrix regulatory proteins in human atherosclerotic plaques. Arteriosclerosis, Thrombosis, and Vascular Biology, 21, 1998–2003.CrossRefGoogle ScholarPubMed
Eichert, D., Salome, M., Banu, M., Susini, J. and Rey, C. (2005) Preliminary characterization of calcium chemical environment in apatitic and non-apatitic calcium phosphates of biological interest by X-ray absorption spectroscopy. Spectrochimica Acta Part B: Atomic Spectroscopy, 60, 850–858.CrossRefGoogle Scholar
Gibson, I.R. and Bonfield, W. (2002) Novel synthesis and characterization of an AB-type carbonatesubstituted hydroxyapatite. Journal of Biomedical Materials Research, 59, 697–708.CrossRefGoogle ScholarPubMed
Hamm, P., Lim, M.H. and Hochstrasser, R.M. (1998) Structure of the amide I band of peptides measured by femtosecond nonlinear-infrared spectroscopy. Journal of Physical Chemistry B, 102, 6123–6138.CrossRefGoogle Scholar
Iyemere, V.P., Proudfoot, D., Weissberg, P.L. and Shanahan, C.M. (2006) Vascular smooth muscle cell phenotypic plasticity and the regulation of vascular calcification. Journal of Internal Medicine, 260, 192–210.CrossRefGoogle ScholarPubMed
Jackson, M., Choo, L.-Pi., Watson, P.H., Halliday, W.C. and Mantsch, H.H. (1995) Beware of connective tissue proteins: Assignment and implications of collagen absorptions in infrared spectra of human tissues. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 1270, 1–6.CrossRefGoogle ScholarPubMed
Jeziorska, M., McCollum, C. and Woolley, D.E. (1998) Calcification in atherosclerotic plaque of human carotid arteries: Associations with mast cells and macrophages. Journal of Pathology, 185, 10–17.3.0.CO;2-0>CrossRefGoogle ScholarPubMed
Johnsson, M.S.A. and Nancollas, G.H. (1992) The role of brushite and octacalcium phosphate in apatite formation. Critical Reviews in Oral Biology & Medicine, 3, 61–82.CrossRefGoogle ScholarPubMed
Kips, J.G., Segers, P. and Van Bortel, L.M. (2008) Identifying the vulnerable plaque: A review of invasive and non-invasive imaging modalities. Artery Research, 2, 21–34.CrossRefGoogle Scholar
Landi, E., Celotti, G., Logroscino, G. and Tampieri, A. (2003) Carbonated hydroxyapatite as bone substitute. Journal of the European Ceramic Society, 23, 2931–2937.CrossRefGoogle Scholar
LeGeros, R.Z., Daculsi, G., Orly, I., Abergas, T. and Torres, W. (1989). Solution-mediated transformation of octacalcium phosphate (OCP) to apatite. Scanning Microscopy, 3, 129–137.Google Scholar
Milev, A., Kannangara, G.S.K. and Ben-Nissan, B. (2003) Morphological stability of hydroxyapatite precursor. Materials Letters, 57, 1960–1965.CrossRefGoogle Scholar
Mohler, E.R. III, Gannon, F., Reynolds, C., Zimmerman, R., Keane, M.G. and Kaplan, F.S. (2001) Bone formation and inflammation in cardiac valves. Circulation, 103, 1522–1528.CrossRefGoogle ScholarPubMed
Movasaghi, Z., Rehman, S. and Rehman, I. (2008) Fourier transform infrared (FTIR) spectroscopy of biological tissues. Applied Spectroscopy Reviews, 43, 134–179.CrossRefGoogle Scholar
Myneni, S.C., Traina, S.J., Waychunas, G.A. and Logan, T.J. (1998) Experimental and theoretical vibrational spectroscopic evaluation of arsenate coordination in aqueous solutions, solids, and at mineral-water interfaces. Geochimica et Cosmochimica Acta, 62(19), 3285–3300.CrossRefGoogle Scholar
Neogi, T., Ellison, R.C., Hunt, S., Terkeltaub, R., Felson, D.T. and Zhang, Y. (2009) Serum uric acid is associated with carotid plaques: the National Heart, Lung, and Blood Institute Family Heart Study. The Journal of Rheumatology, 36(2), 378–384.CrossRefGoogle ScholarPubMed
Pham, P.H., Rao, D.S., Vasunilashorn, F., Fishbein, M.C. and Goldin, J.G. (2006) Computed tomography calcium quantification as a measure of atherosclerotic plaque morphology and stability. Investigative Radiology, 41, 674–680.CrossRefGoogle ScholarPubMed
Richardson, P.D., Davies, M.J. and Born, G.V.R. (1989) Influence of plaque configuration and stress-distribution on fissuring of coronary atherosclerotic plaques. The Lancet, 2, 941–944.CrossRefGoogle ScholarPubMed
Roggli, V.L., Pratt, P.C. and Brody, A.R. (1986) Asbestos content of lung tissue in asbestos associated diseases: a study of 110 cases. British Journal of Industrial Medicine, 43, 18–28.Google ScholarPubMed
Sauer, G.R. and Wuthier, R.E. (1988) Fourier transform infrared characterization of mineral phases formed during induction of mineralization by collagenasereleased matrix vesicles in vitro. Journal of Biological Chemistry, 263, 13718–13724.CrossRefGoogle ScholarPubMed
Scatena, L.F., Brown, M.G. and Richmond, G.L. (2001) Water at hydrophobic surfaces: weak hydrogen bonding and strong orientation effects. Science, 292(5518), 908–912.CrossRefGoogle Scholar
Severinghaus, J.W. (1958) Oxyhemoglobin dissociation curve correction for temperature and pH variation in human blood. Journal of Applied Physiology, 12(3), 485–486.CrossRefGoogle ScholarPubMed
Shah, P.K. (2003) Mechanisms of plaque vulnerability and rupture. Journal of the American College of Cardiology, 41, 15S–22S.CrossRefGoogle ScholarPubMed
Shioi, A., Mori, K., Jono, S., Wakikawa, T., Hiura, Y., Koyama, H., Okuno, Y., Nishizawa, Y. and Morii, H. (2000) Mechanism of atherosclerotic calcification. Zeitschrift für Kardiologie, 89, 75–79.CrossRefGoogle ScholarPubMed
Sofia, S., McCarthy, M.B., Gronowicz, G. and Kaplan, D.L. (2001) Functionalized silk-based biomaterials for bone formation. Journal of Biomedical Materials Research, 54, 139–148.3.0.CO;2-7>CrossRefGoogle ScholarPubMed
Trommer, R.M., Santos, L.A. and Bergmann, C.P. (2009) Nanostructured hydroxyapatite powders produced by a flame-based technique. Materials Science and Engineering: C, 29, 1770–1775.CrossRefGoogle Scholar
Wang, A.Y.M., Ho, S.S.Y., Wang, M., Liu, E.K.H., Ho, S., Li, P.K.T., Lui, S.F. and Sanderson, J.E. (2005) Cardiac valvular calcification as a marker of atherosclerosis and arterial calcification in end-stage renal disease. Archives of Internal Medicine, 165, 327–332.CrossRefGoogle ScholarPubMed
Xin, R.L., Leng, Y. and Wang, N. (2006) In situ TEM examinations of octacalcium phosphate to hydroxyapatite transformation. Journal of Crystal Growth, 289, 339–344.CrossRefGoogle Scholar