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A method for determination of the total dissolved free fatty-acid content of sea water

Published online by Cambridge University Press:  11 May 2009

Paul Treguer
Affiliation:
Groupe d'Océanologie Chimique, Université de Bretagne Occidentale, Avenue Le Gorgeu, 29 N, Brest, France
Pierre Le Corre
Affiliation:
Groupe d'Océanologie Chimique, Université de Bretagne Occidentale, Avenue Le Gorgeu, 29 N, Brest, France
Pierre Courtot
Affiliation:
Groupe d'Océanologie Chimique, Université de Bretagne Occidentale, Avenue Le Gorgeu, 29 N, Brest, France

Extract

A new method is proposed for the determination of total dissolved free fatty acids (FFA) in sea water. After preliminary extraction by chloroform, a copper complex is formed which is then extracted by a chloroform-heptane solution. Copper concentration is measured by atomic absorption spectrophotometry. The method is particularly adapted to the determination of long chain FFA (longer than C10) and dissolved organic matter usually present in sea water gives no interference. At the 30 μ/l. level the standard deviation is 1 μ/l. for seven determinations.

Type
Research Article
Copyright
Copyright © Marine Biological Association of the United Kingdom 1972

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References

Copin, G. & Barbier, M. 1971. Substances organiques dissoutes dans l'eau de mer: premiers résultats de leur fractionnement. Cah. océanogr., Vol. 23, pp. 455–64.Google Scholar
Courtot-Coupez, J. & Le Bihan, A. 1969. Dosage des traces de détergents non-ioniques dissous par spectrophotometrie d'absorption atomique. Analyt. Letters T. 2, pp. 567–76.CrossRefGoogle Scholar
Duncombe, W. G. 1962. The colorimetric determination of long-chain fatty acids in the 0·05–0·5 μmole range. Biochem. J., Vol. 83, p. 6p.Google Scholar
Duncombe, W. G. 1963. The colorimetric micro-determination of long chain fatty acids. Biochem. J., Vol. 88, pp. 710.CrossRefGoogle ScholarPubMed
Duncombe, W. G. 1964. The colorimetric micro-determination of non-esterified fatty acids in plasma. Clinica chim. Acta, Vol. 9, pp. 122–5.CrossRefGoogle ScholarPubMed
Garrett, D. W. 1967. The organic chemical composition of the ocean surface. Deep-Sea Res., Vol. 14. pp. 221–7.Google Scholar
Ho, R. J. & Meng, H. C. 1969. A simple and ultrasensitive method for determination of free fatty acids by radiochemical assay. Analyt. Biochem., Vol. 31, pp. 426–36.CrossRefGoogle ScholarPubMed
Ho, R. J. 1970. Radiochemical assay of long chain fatty acids using Ni 63 as a tracer. Analyt. Biochem., Vol. 36, pp. 105–13.CrossRefGoogle Scholar
Itaya, K. & Ui, M. 1965. Colorimetric determination of free fatty acids in biological fluids. J. Lipid. Res., Vol. 6, pp. 1620.CrossRefGoogle ScholarPubMed
Itaya, K. & Kadowaki, T. 1969. An improved method for the colorimetric determination of free fatty acids. Clinica chim. Acta, Vol. 26, pp. 401–4.Google ScholarPubMed
Iwayama, Y. 1959. New colorimetric determination of higher fatty acids. J. pharm. Soc. Japan., Vol. 79, pp. 552–4.CrossRefGoogle Scholar
Jeffrey, L. M.Pasby, B. F.Stevenson, B. & Hood, D. W. 1964. Lipids of ocean water. Int. Ser. Monographs Earth Sci., Vol. 15, pp. 175–7.Google Scholar
Jeffrey, L. M. 1966. Lipids in sea water. J. Amer. Oil. Chem. Soc., Vol. 43, pp. 211–14.CrossRefGoogle Scholar
Laurell, S. & Tibbling, G. 1967. Colorimetric microdetermination of free fatty acids in plasma. Clinica chim. Acta, Vol. 16, pp. 5762.CrossRefGoogle ScholarPubMed
Le Bihan, A. & Courtot-Coupez, J. 1970. Dosage de traces de détergents anioniques et cationiques dans l'eau de mer et les eaux douces par spectrophotométrie d'absorption atomique. Bull. Soc. chim. Fr., No. 1, pp. 406–11.Google Scholar
Menzel, D. W. & Vaccarro, R. F. 1964. The measurement of dissolved organic and particulate carbon in sea water. Limnol. Oceanogr., Vol. 9, pp. 138–42.CrossRefGoogle Scholar
Novak, M. 1965. Colorimetric ultramicromethod for the determination of free fatty acids. J. Lipid Res., Vol. 6, pp. 431–3.CrossRefGoogle Scholar
Parijs, J. 1966. Spécificité du dosage des acides gras non-estérifiés selon la méthode colorimétrique de Novak. Clinica chim. Acta, Vol. 14, pp. 713–14.CrossRefGoogle ScholarPubMed
Quinn, J. G. & Meyers, P. A. 1971. Retention of dissolved organic acids in sea water by various filters. Limnol. Oceanogr., Vol. 16, pp. 129–31.CrossRefGoogle Scholar
Riley, J. P. & Taylor, D. 1969. The analytical concentration of traces of dissolved organic material from sea water with Amberlite XAD-1 resin. Analyt. chim. Acta, Vol. 46, pp. 307–9.CrossRefGoogle ScholarPubMed
Slavin, W. 1968. Atomic Absorption Spectroscopy. 307 pp. New York: Interscience.Google Scholar
Slowey, J. F.Jeffrey, L. M. & Hood, D. W. 1962. The fatty acid content of ocean water. Geochim. cosmochim. Acta, Vol. 26, pp. 607–16.CrossRefGoogle Scholar
Slowey, J. F.Jeffrey, L. M. & Hood, D. W. 1967. Evidence for organic complexed copper in sea water. Nature, Lond., Vol. 214, pp. 377–8.CrossRefGoogle Scholar
Treguer, P. & Courtot, P. 1969. Paper presented to the Société Chimique de France meeting in Brest. Unpublished results.Google Scholar
Williams, P. M. 1961. Organic acids in pacific ocean waters. Nature, Lond., Vol. 189, No. 4760, pp. 219–20.CrossRefGoogle Scholar
Williams, P. M. 1965. Fatty acids derived from lipids of marine origin. J. Fish. Res. Bd Can., Vol. 22, pp. 1107–22.CrossRefGoogle Scholar