Hostname: page-component-848d4c4894-2xdlg Total loading time: 0 Render date: 2024-06-30T11:46:20.707Z Has data issue: false hasContentIssue false
  • English
  • Français

New insight into the diagnostic cut-off value of serum anti-Toxocara IgG for ocular toxocariasis in uveitis patients

Published online by Cambridge University Press:  20 November 2018

N.F. Abd El-Aal Open the ORCID record for N.F. Abd El-Aal [Opens in a new window] ,
M.A.A. Basha  and
A.M. Eid
N.F. Abd El-Aal*
Affiliation:
Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Egypt
M.A.A. Basha
Affiliation:
Department of Radiology, Faculty of Medicine, Zagazig University, Egypt
A.M. Eid
Affiliation:
Department of Ophthalmology, Faculty of Medicine, Zagazig University, Egypt
*
Author for correspondence: N.F. Abd El-Aal E-mail: naglaa_fathy220@yahoo.com
Get access Rights & Permissions [Opens in a new window]

Abstract

Uveitis is one of the commonest causes of vision loss worldwide and its exact etiology is still not clarified in most patients. The current study is a trial to assess the efficacy of serum anti-Toxocara immunoglobulin G (IgG) by enzyme-linked immunosorbent assay (ELISA) as a diagnostic tool for ocular toxocariasis (OT) and to detect OT prevalence and the associated ocular manifestations in sera of patients with uveitis. One hundred and twelve patients (62 females and 50 males) with uveitis were diagnosed by ophthalmologists, radiologists and rheumatologists according to ocular manifestations, laboratory and radiological investigations. Serum anti-Toxocara IgG titers were determined by ELISA in sera of all patients. Our results revealed that OT is highly associated with intermediate and posterior uveitis. Children and young adult females, especially those residing in rural areas, complained mainly of diminution of vision in the left eye, with strabismus and leukocoria. At a cut-off value of 0.258, the sensitivity and specificity of IgG ELISA were 93.3% and 100%, respectively. In conclusion, at a novel cut-off value of 0.258 the serum anti-Toxocara IgG ELISA is predicted to be a diagnostic tool for OT regarding sensitivity and specificity. Also, it has potential importance in the interpretation and differential diagnosis of OT. Thus, serum anti-Toxocara IgG ELISA should be a routine test for screening of suspected cases.

Keywords

ELISAocularseroprevalencetoxocariasisuveitis
Type
Research Paper
Information
Journal of Helminthology , Volume 94 , 2020 , e12
Copyright
Copyright © Cambridge University Press 2018 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Ahn, SJ, Ryoo, NK and Woom, SJ (2014a) Ocular toxocariasis: clinical features, diagnosis, treatment, and prevention. Asia Pacific Allergy 4,134–141.Google Scholar
Ahn, SJ et al. (2014b) Clinical features and course of ocular toxocariasis in adults. PLoS Neglected Tropical Diseases 8, e2938.Google Scholar
Bae, KW et al. (2016) Diagnostic value of the serum anti-Toxocara IgG titer for ocular toxocariasis in patients with uveitis at a tertiary hospital in Korea. Korean Journal of Ophthalmology 30, 258–264.Google Scholar
Benitez del Castillo, JM et al. (1995) Bilateral ocular toxocariasis demonstrated by aqueous humor enzyme-linked immunosorbent assay. American Journal of Ophthalmology 119, 514–516.Google Scholar
Cilla, G et al. (1996) Seroprevalence of Toxocara infection in middle-class and disadvantaged children in northern Spain (Gipuzkoa, Basque Country). European Journal of Epidemiology 12, 541–543.Google Scholar
Cortez, RT et al. (2011) Ocular parasitic diseases: a review on toxocariasis and diffuse unilateral subacute neuroretinitis. Journal of Pediatric Ophthalmology and Strabismus 48, 204–212.Google Scholar
Despommier, D (2003) Toxocariasis: clinical aspects, epidemiology, medical ecology, and molecular aspects. Clinical Microbiology Reviews 16, 265–272.Google Scholar
Hotez, PJ and Wilkins, PP (2009) Toxocariasis: America's most common neglected infection of poverty and a helminthiasis of global importance? PLoS Neglected Tropical Diseases 3, e400.Google Scholar
Jin, Y et al. (2013) Serodiagnosis of toxocariasis by ELISA using crude antigen of Toxocara canis larvae. Korean Journal of Parasitology 51, 433–439.Google Scholar
Kwon, J, Sim, Y and Jee, D (2017) Association between intermediate uveitis and toxocariasis in the Korean population. Medicine 96, e5829.Google Scholar
Magnaval, JF et al. (2001) Highlights of human toxocariasis. Korean Journal of Parasitology 39, 1–11.Google Scholar
Noordin, R et al. (2005) Comparison of IgG-ELISA and IgG4-ELISA for toxocara serodiagnosis. Acta Tropica 93, 57–62.Google Scholar
Paroli, MP et al. (2014) Intermediate uveitis: comparison between childhood-onset and adult-onset disease. European Journal of Ophthalmology 24, 94–100.Google Scholar
Rubinsky-Elefant, G et al. (2010) Human toxocariasis: diagnosis, worldwide seroprevalences and clinical expression of the systemic and ocular forms. Annals of Tropical Medicine and Parasitology 104, 3–23.Google Scholar
Rubinsky-Elefant, G et al. (2018) Toxocariasis: critical analysis of serology in patients attending a public referral center for ophthalmology in Brazil. Japanese Journal of Ophthalmology 62, 77–83.Google Scholar
Schantz, PM (1989) Toxocara larva migrans now. American Journal of Tropical Medicine and Hygiene 41, 21–34.Google Scholar
Schantz, PM (1994) Of worms, dogs, and human hosts: continuing challenges for veterinarians in prevention of human disease. Journal of the American Veterinary Medicine Association 204, 1023–1028.Google Scholar
Smith, HV et al. (2009) How common is human toxocariasis? Towards standardizing our knowledge. Trends in Parasitology 25, 182–188.Google Scholar
Taylor, MR (2001) The epidemiology of ocular toxocariasis. Journal of Helminthology 75, 109–118.Google Scholar
Yoshida, M et al. (1999) A retrospective study of ocular toxocariasis in Japan, correlation with antibody prevalence and ophthalmological findings of patients with uveitis. Journal of Helminthology 73, 357–361.Google Scholar