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Cytochemical observations on the nervous system of adult Corrigia vitta

Published online by Cambridge University Press:  05 June 2009

C. A. Magee ,
M. Cahir ,
D. W. Halton ,
C. F. Johnston  and
C. Shaw
C. A. Magee
Affiliation:
Comparative Neuroendocrinology Research Group, Schools of Biology & Biochemistry and Clinical Medicine, The Queen's University of Belfast, Belfast BT7 INN, UK
M. Cahir
Affiliation:
Comparative Neuroendocrinology Research Group, Schools of Biology & Biochemistry and Clinical Medicine, The Queen's University of Belfast, Belfast BT7 INN, UK
D. W. Halton*
Affiliation:
Comparative Neuroendocrinology Research Group, Schools of Biology & Biochemistry and Clinical Medicine, The Queen's University of Belfast, Belfast BT7 INN, UK
C. F. Johnston
Affiliation:
Comparative Neuroendocrinology Research Group, Schools of Biology & Biochemistry and Clinical Medicine, The Queen's University of Belfast, Belfast BT7 INN, UK
C. Shaw
Affiliation:
Comparative Neuroendocrinology Research Group, Schools of Biology & Biochemistry and Clinical Medicine, The Queen's University of Belfast, Belfast BT7 INN, UK
*
*Author to whom correspondence should be addressed.
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Abstract

Adult Corrigia vitta (Trematoda: Dicrocoelidea) inhabit the pancreatic duct of the fieldmouse, Apodemus sylvaticus, where, in numbers, they may occlude the duct lumen and prevent the flow of pancreatic secretions. Enzyme histochemical and immunocytochemical techniques, in conjunction with confocal scanning laser microscopy, have been used to examine the localization and distribution of cholinergic. serotoninergic (5-HT, serotonin) and peptidergic components of the nervous system of the adult worm. All three classes of neuronal mediator showed a common pattern of staining, occurring throughout the central and peripheral nervous systems. Of the four peptide immunoreactivities (IR) demonstrated (pancreatic polypeptide (PP), peptide YY (PYY), substance P (SP), FMRFamide), PP-IR was the most predominant, occurring not only within the central ganglia and longitudinal nerve cords, but also in subtegumental plexuses and in fibres associated with the egg-forming apparatus. PYY and FMRFamide IRs were evident throughout the central and peripheral nervous systems; FMRFamide immunostaining, in particular, highlighted innervation of the ootype and immunoreactive cell bodies around the Mehlis' gland. Both SP- and 5-HT-IRs were restricted to the cerebral ganglia, ventral nerve cords and associated cell bodies. The distribution pattems of these peptides and 5-HT within the nervous system of C. vitta suggest they are likely to function as neuronal mediators. PP, PYY and FMRFamide may also serve in regulating egg production.

Keywords

Corrigia villaTrematodaneuropeptidesimmunocytochemistryconfocal scanning laser microscopypancreatic polypeptidepeptide YYFMRFamidesubstance P5-hydroxytryptamineserotonincholinesterase
Type
Research Papers
Information
Journal of Helminthology , Volume 67 , Issue 3 , September 1993 , pp. 189 - 199
Copyright
Copyright © Cambridge University Press 1993

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