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Comparative study of the separation of casein from bovine, ovine and caprine milks using HPLC

Published online by Cambridge University Press:  01 June 2009

Stelios E. Kaminarides  and
Emmanuel M. Anifantakis
Stelios E. Kaminarides
Affiliation:
Agricultural University of Athens, lera Odos 75, Votanikos, 11855 Athens, Greece
Emmanuel M. Anifantakis
Affiliation:
Agricultural University of Athens, lera Odos 75, Votanikos, 11855 Athens, Greece
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Summary

The separation of cows', sheep's and goats' milk casein components by HPLC on a strong anion-exchange (P.L-SAX 8μ 1000A) column is described. During HLPC, whole caseins of the three kinds of milk behaved differently from conventional separations. The casein components of the three kinds of milk were well resolved under the Chromatographie conditions used. HPLC resolved the κ-caseins better than did ion-exchange chromatography on DEAE cellulose, and was particularly efficient in the case of goats' milk. Goats' and sheep's milks had almost similar Chromatographie profiles but these differed considerably from that of cows' milk. Caseins from the sheep and the goat were also similar in that a shallower NaCl gradient was required for the separation of casein components than for cows' milk.

Type
Original Articles
Information
Journal of Dairy Research , Volume 60 , Issue 4 , November 1993 , pp. 495 - 504
Copyright
Copyright © Proprietors of Journal of Dairy Research 1993

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References

REFERENCES

Addeo, F., Mauriello, L., Moio, L., Laezza, P., Chianese, L. & Di Luccia, A. 1992 Ovine casein variant identification using electrophoretic, immunochemical and Chromatographie techniques. Milchwissenschaft 47 283–287Google Scholar
Addeo, F., Soulier, S., Pelissier, J.-P., Chobert, J.-M., Mercier, J.-C., & Ribadeau-Dumas, B. 1978 Preparation and fractionation of goat κ-casein: analysis of the glycan and peptide components. Journal of Dairy Research 45 191–196CrossRefGoogle ScholarPubMed
Andrews, A. T. & Alichanidis, E. 1983 Proteolysis of caseins and the proteose-peptone fraction of bovine milk. Journal of Dairy Research 50 275–290CrossRefGoogle ScholarPubMed
Andrews, A. T., Taylor, M. D. & Owen, A. J. 1985 Rapid analysis of bovine milk proteins by fast protein liquid chromatography. Journal of Chromatography 348 177–185CrossRefGoogle ScholarPubMed
Collin, J. C., Kokelaar, A., Rollet-Repecaud, O. & De Lacroix-Buchet, A. 1991 [Determination of caseins in cows' milk by electrophoresis and fast protein liquid chromatography (FPLC): comparison of results.] Lait 71 339–350CrossRefGoogle Scholar
Dall'Olio, S., Davoli, R. & Tedeschi, M. 1988 [A contribution to the study of goat casein by chromatography.] Scienza e Tecnica Lattiero-casearia 39 167–178Google Scholar
Davies, D. T. & Law, J. R. 1977 An improved method for the quantitative fractionation of casein mixtures using ion-exchange chromatography. Journal of Dairy Research 44 213–221CrossRefGoogle Scholar
Davies, D. T. & Law, A. J. R. 1987 Quantitative fractionation of casein mixtures by fast protein liquid chromatography. Journal of Dairy Research 54 369–376CrossRefGoogle Scholar
De Jong, L. 1975 A quantitative electrophoretic method of studying cheese ripening. Netherlands Mille and Dairy Journal 29 162–168Google Scholar
Dimenna, G. P. & Segall, H. J. 1981 High performance gel-permeation chromatography of bovine skim milk proteins. Journal of Liquid Chromatography 4 639–649CrossRefGoogle Scholar
Haasnoot, W., Venema, D. P. & Elenbaas, H. L. 1986 Determination of cow milk in the milk and cheese and ewes and goats by fast protein liquid chromatography. Milchwissenschaft 41 642–645Google Scholar
Mercier, J. C., Maubois, J. L., Poznanski, S. & Ribadeau-Dumas, B. 1968 [Preparative fractionation of cow and sheep casein by chromatography on DEAE-cellulose with urea and 2-mercaptoethanol.] Bulletin de la Société de Chimie Biologique 50 521–530Google ScholarPubMed
Richardson, B. C. & Creamer, L. K. 1974 Comparative micelle structure. 3. The isolation and chemical characterization of caprine β1-casein and β2-casein. Biochimica et Biophysica Acta, 365 133–137CrossRefGoogle Scholar
Richardson, B. C. & Creamer, L. K. 1976 Comparative micelle structure. 5. The isolation and characterization of the major ovine caseins. New Zealand Journal of Dairy Science and Technology 11 46–53Google Scholar
Richardson, B. C., Creamer, L. K. & Munford, R. E. 1973 Comparative micelle structure. 1. The isolation and chemical characterization of caprine κ-casein. Biochimica et Biophysica Acta 310 111–117CrossRefGoogle ScholarPubMed
Richardson, B. C., Creamer, L. K. & Munford, R. E. 1974 The composition and properties of caprine (goat) caseins. 19th International Dairy Congress, New Delhi IE 268Google Scholar
Richardson, B. C. & Mercier, J. C. 1979 The primary structure of the ovine β-caseins. European Journal of Biochemistry 99 285–297Google ScholarPubMed
Visser, S., Slangen, K. J. & Rollema, H. S. 1986 High-performance liquid chromatography of bovine caseins with the application of various stationary phases. Milchwissenschaft 41 559–562Google Scholar
Zittle, C. A. & Custer, J. H. 1963 Purification and some properties of αs-casein and κ-casein. Journal of Dairy Science 46 1183–1188CrossRefGoogle Scholar