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Quantitation and analysis of the specificity of post-immunization antibodies to influenza B viruses using single radial haemolysis

Published online by Cambridge University Press:  25 March 2010

J. S. Oxford ,
R. Yetts  and
G. C. Schild
J. S. Oxford
Affiliation:
Division of Virology, National Institute for Biological Standards and Control, Holly Hill, London NW3 6RB
R. Yetts
Affiliation:
Division of Virology, National Institute for Biological Standards and Control, Holly Hill, London NW3 6RB
G. C. Schild
Affiliation:
Division of Virology, National Institute for Biological Standards and Control, Holly Hill, London NW3 6RB
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Summary

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The single radial haemolysis (SRH) technique detected anti-B/HK/8/73 HA antibody rises in 59–85 % of paired sera from persons immunized with different influenza vaccines. In contrast, analysis of the same sera by the haemagglutination inhibition (HI) test indicated significant antibody rises in only 27–54% of paired sera. High levels of antibody were detected to influenza B/HK/8/73 and B/Singapore/222/79 viruses in post-immunization sera analysed by SRH, whereas the HI test indicated comparatively low geometric mean antibody titres. Most adults responded to immunization with influenza B virus by producing cross-reactive (CR) antibody which reacted with different influenza B viruses including the early isolate B/Lee/40.

Type
Research Article
Information
Journal of Hygiene , Volume 88 , Issue 2 , April 1982 , pp. 325 - 333
Copyright
Copyright © Cambridge University Press 1982

References

REFERENCES

Brand, C. M. & Skehel, J. J. (1972). Crystalline antigen from influenza virus envelope. Nature 238, 145.Google ScholarPubMed
Chakraverty, P. (1971). Antigenic relationships between influenza B viruses. Bulletin of the World Health Organisation 45, 755.Google ScholarPubMed
Chakraverty, P. (1980). Comparison of haemagglutination-inhibition and single radial haemolysis techniques for detection of antibodies to influenza B virus. Archives of Virology 63, 285.CrossRefGoogle ScholarPubMed
Couch, R. B., Webster, R. G., Kasel, J. A. & Cate, T. R. (1979). Efficacy of purified influenza subunit vaccines and relation to the major antigenic determinants in the haemagglutinin molecule. Journal of Infectious Diseases 14, 553.CrossRefGoogle Scholar
Davenport, F. M., Hennessey, A. V. & Francis, T. (1953). Epidemiologic and immunologic significance of age distribution of antibody to antigens of variants of influenza virus. Journal of Experimental Medicine 98, 641.CrossRefGoogle ScholarPubMed
Fazekas de St Groth, S. & Webster, R. G. (1966). Disquisitions on original antigenic sin. I. Evidence in man. Journal of Experimental Medicine 124, 331.CrossRefGoogle Scholar
Francis, T. (1953). Influenza: the newe acquayntance. Annals of Internal Medicine 39, 203.Google Scholar
La Montagne, J. R. (1980). Summary of a workshop on influenza B viruses and Reyes Syndrome. Journal of Infectious Diseases 142, 452.CrossRefGoogle Scholar
Luzyanina, Y., Nevedomskaya, G. N., Taros, L. Yu., Kudryavtseva, V. K., Smorodintsev, A. A. (1979). Antigenic relationships between strains of influenza B virus Acta virologica 23, 113.Google ScholarPubMed
Monto, A. S. & Maassab, H. F. (1981). Ether treatment of type B influenza antigen for the haemagglutination inhibition test. Journal of Clinical Microbiology 13, 54.CrossRefGoogle Scholar
Oxford, J. S., Schild, G. C., Potter, C. W. & Jennings, R. (1979). The specificity of anti-haemagglutinin antibody response induced in man by inactivated influenza vaccines and by natural infection. Journal of Hygiene 82, 51.CrossRefGoogle Scholar
Oxford, J. S., Haaheim, L. R., Slepushkin, A., Werner, J., Kuwert, E. & Schild, G. C. (1981). Strain specificity of serum antibody to the haemagglutinin of influenza A (H3N2) viruses in children following immunization or natural infection. Journal of Hygiene 86, 17.CrossRefGoogle ScholarPubMed
Schild, G. C., Pereira, M. S., Chakraverty, P., Coleman, M. T., Dowdle, W. R. & Chang, W. K. (1973). Antigenic variants of influenza B virus. British Medical Journal iv, 127.CrossRefGoogle Scholar
Schild, G. C., Pereira, M. S. & Chakraverty, P. (1975). Single radial haemolysis: a new method for the assay of antibody to influenza haemagglutinin. Bulletin of the World Health Organization 52, 43.Google ScholarPubMed
Schild, G. C., Oxford, J. S. & Virelizier, J. L. (1976). In ʻThe Role of Immunological Factors in Infectious, Allergic and Autoimmune Processes (ed. Beers, R. F. and Bassett, E. C.), p. 481. New York: Raven Press.Google Scholar
Schild, G. C.Smith, J. W. G., Cretescu, L.Newman, R. W. & Wood, J. M. (1977). Strain specificity of antibody to haemagglutinin following inactivated A/PC/73 vaccine in man: evidence for a paradoxical strain-specific antibody response. Developments in Biological Standardization 39, 273.Google Scholar
Skehel, J. J. & Schild, G. C. (1971). The polypeptide composition of influenza A viruses. Virology 44, 396.CrossRefGoogle ScholarPubMed
Stuart-Harris, C. H. & Schild, G. C. (1976). In Influenza: the Viruses and the Disease. London: Edward Arnold.Google Scholar
Vaananen, P., Hovi, T.Helle, E. P. & Penttinen, K. (1976). Determination of mumps and influenza antibodies by haemolysis in gel. Archives of Virology 52, 91.CrossRefGoogle ScholarPubMed
Weekly Epidemiological Record (1980). 55, 73.Google Scholar
Wright, P. F., Bryant, J. D. & Karzon, D. T. (1980). Comparison of influenza B/Hong Kong virus infections among infants, children and young adults. Journal of Infectious Diseases 141, 430.CrossRefGoogle ScholarPubMed