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Identification and characterization of anonymous nuclear markers for the double-striped cockroach, Blattella bisignata

Published online by Cambridge University Press:  15 June 2012

Q.-P. Ren ,
Z. Fan ,
X.-M. Zhou ,
G.-F. Jiang ,
Y.-T. Wang  and
Y.-X. Liu
Q.-P. Ren
Affiliation:
Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210046, China
Z. Fan
Affiliation:
Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210046, China
X.-M. Zhou
Affiliation:
Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210046, China
G.-F. Jiang*
Affiliation:
Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210046, China
Y.-T. Wang
Affiliation:
Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210046, China
Y.-X. Liu
Affiliation:
Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210046, China
*
*Author for correspondence Fax: +86-2585891163 E-mail: cnjgf1208@gmail.com, cnjgf1208@163.com
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Abstract

During the last decade, multilocus analysis has gradually become a powerful tool for the studies of population genetics and phylogeography. The double-striped cockroach, Blattella bisignata, is endemic to southeast Asia, and there is currently little genetic information available for the species. We chose it as the target species to investigate a biodiversity hotspot in southwest China. Here, we report the identification and characterization of 11 single-copy anonymous nuclear markers with an average length of 378bp. These loci, isolated from a genomic library of B. bisignata, can amplify in two additional Blattella species (B. germanica and B. lituricollis). While testing these markers in representative species of Blattellidae, Blattidae and Epilampridae, some of them can cross-amplify successfully. After sequencing 30 individuals collected from southern China per locus, we found relatively high variability (approximately 3.6 SNPs per 100bp). Finally, a small-scale study was also performed to show that these markers do indeed fulfill the expectations as phylogeographic markers.

Keywords

Blattella bisignatasingle-copy nuclear polymorphic markerscross-species amplificationgenetic diversityphylogeography
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 103 , Issue 1 , February 2013 , pp. 29 - 35
Copyright
Copyright © Cambridge University Press 2012

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