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This study aimed to clarify the association between both hypoxia-inducible factor-1α and glucose transporter type-1 expression and survival outcome in advanced pharyngeal cancer without human papillomavirus infection.
Twenty-five oropharyngeal and 55 hypopharyngeal cancer patients without human papillomavirus infection were enrolled. All patients had stage III–IV lesions and underwent concurrent chemoradiotherapy or surgery. Hypoxia-inducible factor-1α and glucose transporter type-1 expression were investigated in primary lesions by immunohistochemistry.
There were 41 and 39 cases with low and high hypoxia-inducible factor-1α expression, and 28 and 52 cases with low and high glucose transporter type-1 expression, respectively. There was no significant correlation between hypoxia-inducible factor-1α and glucose transporter type-1 expression. In univariate analysis, nodal metastasis, clinical stage and high hypoxia-inducible factor-1α expression, but not glucose transporter type-1 expression, predicted significantly worse prognosis. In multivariate analysis, hypoxia-inducible factor-1α overexpression was significantly correlated with poor overall survival, disease-specific survival and recurrence-free survival.
High hypoxia-inducible factor-1α expression was an independent risk factor for poor prognosis for advanced human papillomavirus-unrelated pharyngeal cancer.
The effects of iron deficiency on the prognosis of idiopathic sudden sensorineural hearing loss are unclear. This study aimed to investigate the association between serum iron levels and idiopathic sudden sensorineural hearing loss prognosis and its usefulness as an independent prognostic marker for idiopathic sudden sensorineural hearing loss.
The audiological and haematological data, including hearing recovery and serum iron levels, of 103 patients with idiopathic sudden sensorineural hearing loss evaluated between 2015 and 2018 were retrospectively analysed.
The overall complete recovery rate was 16.5 per cent. Initial higher hearing threshold was associated with poor idiopathic sudden sensorineural hearing loss prognosis. Serum iron levels were significantly higher in the complete recovery group than in the non-complete recovery group (p < 0.05).
The possibility of complete recovery from idiopathic sudden sensorineural hearing loss was significantly lower with lower serum iron levels, suggesting that the serum iron level might be a novel prognostic marker for idiopathic sudden sensorineural hearing loss.
The risk for developing and preserving symptoms of anorexia nervosa (AN) in children seems to be multifactorial: individual, familial, socio-cultural, and biological factors interact within the developmental framework. A disruption of attachment processes with the mother in an early stage of child development has been proposed as a contributing factor; however, the evidence is controversial.
To address the research question of how childhood AN patients recognize disorder-specific provocative factors such as body type, high-calorie food, and attachment between mother and child.
The aim of this study was to investigate the prefrontal activation in childhood AN patients when imaging those symptom-provocative factors.
The prefrontal activations during each task, in terms of blood volume changes, were measured by near infrared spectroscopy. Twelve females with childhood AN (mean age, 14.4 years old) and 13 age-matched healthy female controls participated in this study.
Both groups showed increased prefrontal blood volume when viewing images of each symptom-provocative factor. Unexpectedly, there was no significant difference in the prefrontal blood volume increases between the control group and the childhood AN group when viewing images of slender and obese body types and high-calorie food. On the other hand, images of mother-child attachment resulted in significantly greater increases in prefrontal blood volume in the childhood AN group than in the control group.
These results indicated that prefrontal activation in AN might be associated with imaging attachment between mother and child, but not associated with imaging body type or high-calorie food.
The tachinid fly Drino inconspicuoides (Diptera: Tachinidae) is an ovolarviparous endoparasitoid whose larvae develop in the host haemocoel and avoids the host immune system. In this study, we investigated the immune evasion mechanisms of this species during infestation in the host Mythimna separata (Lepidoptera: Noctuidae). We discovered a unique ‘cloak’ that surrounded D. inconspicuoides larvae that penetrated into the host and determined through genomic polymerase chain reaction analysis that this structure originated from the host rather than the tachinid. The ‘cloak’ contained both haemocytes and fat body cells from the host, with the haemocytes assembling around the larvae first and the fat body cells then covering the haemocyte layer, following which the two mixed. Living D. inconspicuoides larvae that were wrapped in the ‘cloak’ were not melanized whereas encapsulated dead larvae were melanized, suggesting that this structure contributes to the avoidance of host immune reactions.
Cognitive–behavioral therapy (CBT) is thought to be useful for chronic pain, with the pathology of the latter being closely associated with cognitive–emotional components. However, there are few resting-state functional magnetic resonance imaging (R-fMRI) studies. We used the independent component analysis method to examine neural changes after CBT and to assess whether brain regions predict treatment response.
We performed R-fMRI on a group of 29 chronic pain (somatoform pain disorder) patients and 30 age-matched healthy controls (T1). Patients were enrolled in a weekly 12-session group CBT (T2). We assessed selected regions of interest that exhibited differences in intrinsic connectivity network (ICN) connectivity strength between the patients and controls at T1, and compared T1 and T2. We also examined the correlations between treatment effects and rs-fMRI data.
Abnormal ICN connectivity of the orbitofrontal cortex (OFC) and inferior parietal lobule within the dorsal attention network (DAN) and of the paracentral lobule within the sensorimotor network in patients with chronic pain normalized after CBT. Higher ICN connectivity strength in the OFC indicated greater improvements in pain intensity. Furthermore, ICN connectivity strength in the dorsal posterior cingulate cortex (PCC) within the DAN at T1 was negatively correlated with CBT-related clinical improvements.
We conclude that the OFC is crucial for CBT-related improvement of pain intensity, and that the dorsal PCC activation at pretreatment also plays an important role in improvement of clinical symptoms via CBT.
Metal Organic Decomposition (MOD)-made BaTiO3 (BT) thin films were prepared for Resistive Random Access Memory (ReRAM) under various annealing conditions and investigated for improving the properties of bipolar-type resistive switching, focusing on the relation between oxygen vacancies and the behavior of resistive hysteresis. BT thin films with both pre- and final- annealing in nitrogen showed the resistive hysteresis of bipolar-type switching with current ON/OFF ratios of 2 orders of magnitude for both bias polarities. Finally they showed the endurance property with the 106 switching cycles. It was suggested that oxygen vacancies near the oxide surface (both interfaces at metal electrode/oxide and between layer-by-layered oxide layers) are increased by N2 annealing and enhanced the interface-type resistive switching. Pre-annealing in N2 was also found to be very effective to improve endurance properties, implying that not only the electrode/oxide interface but also the middle part of the film would contribute the interface-type mechanism.
We present high resolution molecular line observations of dusty AGN and starburst in nearby luminous infrared galaxies (LIRGs), VV 114 (band 3/4/7) and NGC 1614 (band 3/6/7/9), with ALMA. Multi-frequency imaging from 4.8 GHz to 691 GHz of NGC 1614 allows us to study spatial properties of the radio-to-FIR continuum and multiple CO transitions, and we find the CO excitation up to Jupp = 6 can be explained by a single ISM model powered by nuclear starbursts. Our processing line imaging survey for VV 114 detected at least 30 molecular lines which show different chemical composition from region to region. Multi-molecule imaging helps us to diagnose the chemical differences of dusty ISM, while multi-transition imaging allows us to investigate gas physical conditions affected by nuclear activities directly.
We succeeded in photovoltaic power generation of p-i-n solar cells utilizing epitaxial ZnInON film with a wide band gap of 3.1 eV as the intrinsic layer, suitable for a top cell of tandem solar cells. The solar cell shows a high open circuit voltage (Voc) of 1.68 V under solar simulator light irradiation of 3.2 mW/cm2. The solar cell performance becomes worse under 100 mW/cm2, which is mainly attributed to the leakage current caused by crystal defects and grain boundaries. X-ray diffraction analysis reveals that the ZnInON film has rather large tilt and twist angles and a high dislocation density of 7.62×1010 cm-2. Such low crystallinity is a bottleneck for high performance of the solar cells. Our results demonstrate a potential of epitaxial ZnInON films as an intrinsic layer of wide band gap p-i-n solar cells with a high Voc.
Effects of surface morphology of buffer layers on ZnO/sapphire heteroepitaxial growth have been investigated by means of “nitrogen mediated crystallization (NMC) method”, where the crystal nucleation and growth are controlled by absorbed nitrogen atoms. We found a strong correlation between the height distribution profile of NMC-ZnO buffer layers and the crystal quality of ZnO films. On the buffer layer with a sharp peak in height distribution, a single-crystalline ZnO film with atomically-flat surface was grown. Our results indicate that homogeneous and high-density nucleation at the initial growth stages is critical in heteroepitaxy of ZnO on lattice mismatched substrates.
Two typed bio-conjugated soft-interface for highly sensitive immunoassay was developed by integrating a phospholipid polymer. Nano-sphered surface with poly [2-methacryloyloxyethyl phosphorylcholine (MPC)-co-n-butyl methacrylate (BMA)-co-p-nitrophenyloxycarbonyl poly(ethylene glycol) methacrylate (MEONP)]: PMBN) was prepared by electrospray deposition (ESD) method. The three dimensional nano-sphered surface can be captured an antibody with high density around 860 ng/cm2. The theoretical amount of closest packed immobilized antibodies on flat surface is around 650 ng/cm2, thus large amount of antibodies were immobilized on the nano-sphere surface. The water stability of PMBN nanostructure was improved by crosslinking with 1,4-butylenediamine and by heating. Both heated and cross-linked PMBN nanostructure was not changed at all remaining high porosity after immersing in water. The specific signal in the immunoassay was enhanced with both heated and cross-linked PMBN nanostructure. The PMBN nanostructure which has high porosity and high water stability realized highly sensitive immunoassay.
As the other platform, we developed a novel soft-interface consisting of a well-defined phospholipid polymer surface on which Staphylococcal Protein A (SpA) was site-selectively immobilized. The phospholipid polymer platform was prepared on silicon substrates using the surface-initiated atom transfer radical polymerization (SI-ATRP) technique. Orientation-controlled antibodies were achieved using enzymatic reactions, and these antibodies captured 1.8 ± 0.1 antigens on average, implying that at least 80% of immobilized antibodies reacted with 2 antigens. Theoretical multivalent binding analysis further revealed that orientation-controlled antibodies had antigen-antibody reaction equilibrium dissociation constants (Kd) as low as 8.6 × 10-10 mol/L, whereas randomly oriented and partially oriented antibodies showed Kd values of 2.0 × 10-7 mol/L and 1.2 × 10-7 mol/L, respectively. These findings support the significance of antibody orientation because controlling the orientation resulted in high reactivity and theoretical binding capacity.
A novel allergy biosensor is designed and fabricated by using thin film bulk acoustic resonator (TFBAR) devices with shear mode ZnO piezoelectric thin films. To fabricate TFBAR devices, the off-axis RF magnetron sputtering method for the growth of piezoelectric ZnO piezoelectric thin films is adopted. The influences of the relative distance and sputtering parameters are investigated. In this report, the piezoelectric ZnO thin films with tilting angle are set by controlling the deposition parameters. The properties of the shear mode ZnO thin films are investigated by X-ray diffraction and scanning electron microscopy. The frequency response is measured using an HP8720 network analyzer with a CASCADE probe station. The resonance frequency of the shear mode is 796.75 MHz. The sensitivity of the shear mode is calculated to be 462.5 kHz·cm2/ng.
The sensor team at the Los Alamos National Laboratory is an integrated multidisciplinary group that develops both core technologies as well as accessory tools for efficient biodetection. We have developed a waveguide-based optical biosensor for the efficient and ultra-sensitive, rapid detection of biological agents. We have previously demonstrated the use of this technology for the detection of biomarkers associated with many diseases. Herein, we present the preliminary data demonstrating the extension of this technology to the discovery and detection of Traumatic Brain Injury (TBI). TBI afflicts a significant percentage of US troops deployed in Iraq and Afghanistan, but is difficult to diagnose efficiently. Currently, only neuropsychological questionnaires are being used for the diagnosis of this condition, which can range from mild concussion to severe brain damage. The ultimate goal of this project is to develop a rapid biomarker-based diagnostic for TBI in blood. However, this cannot be accomplished until a comprehensive repertoire of biomarkers secreted during brain injury is established. This requires an integrated biomarker discovery and detection approach that is sampled directly from human serum and cerebrospinal fluid.
The results reported here are preliminary steps in that direction wherein we aim to develop two different methods for the discovery of novel biomarkers of TBI in blood and cerebrospinal fluid, as well as develop assays for two biomarkers on an ultra-sensitive waveguide-based platform that was developed at LANL. We were able to evaluate two different methods for biomarker discovery: Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) and two dimensional gel electrophoresis (2-DE) in serum samples. In addition to development of depletion protocols to remove abundant proteins in serum, we were also able to detect spiked TBI biomarkers using both methods. However, the results clearly show that for protein biomarkers, MALDI MS is much more sensitive than 2-DE. We also developed a sandwich immunoassay on a waveguide-based platform for a TBI biomarker, procalcitonin, using commercially available antibodies. We show with our methods that we were able to directly detect procalcitonin from human serum. While our discovery and detection methods show promising results, these methods need to be further optimized before we can apply it to clinically relevant samples.
Microfluidic mixing was applied to conventional acid pasting process to re-crystallize organic nanocrystals of Titanyl phthalocyanine (TiOPc). TiOPc nanocrystals were re-crystallized in a two step process. Seed particles were prepared by mixing TiOPc, dissolved in concentrated sulfuric acid with deionized water using high speed microchannel mixers. Seed particles were then subjected to post-precipitation treatment to achieve final crystalline product. Effects of seed preparation conditions, such as mixing efficiency (mixer type) and mixing temperature on the structure of final product were studied. Time evolution of optical absorption spectra was examined with a view to elucidate structure evolution during early stages of seed formation process.
In March 1989 a large outbreak of acute gastroenteritis occurred simultaneously among schoolchildren and teachers at nine elementary schools in Toyota City, Japan. Illness was observed in 3236 (41·5%) of 7801 schoolchildren and 117 (39·4%) of 297 teachers. The main clinical symptoms were diarrhoea, vomiting, nausea and abdominal pain. Gastroenteritis was significantly associated with the consumption of school lunch served by one particular lunch preparation centre. One food handler at the centre suffered from gastroenteritis during the outbreak.Small round structured virus (SRSV) was detected in 4 of 8 stool specimens from sick persons. The school lunch contaminated by the infected food handler is the most probable source of this outbreak due to SRSV.
The inhibitory influence of cyanuric acid on the virucidal effect of chlorine was studied. The time required for 99·9% inactivation of ten enteroviruses and two adenoviruses by 0·5 mg/l free available chlorine at pH 7·0 and 25○C was prolonged approximately 4·8–28·8 times by the addition of 30 mg/l cyanuric acid. Comparative inactivation of poliovirus 1 by free available chlorine with or without cyanuric acid revealed the following. The inactivation rate by 1·5 mg/l free available chlorine with 30 mg/l cyanuric acid or by 0·5 mg/l free available chlorine with 1 mg/1 cyanuric acid was slower than by 0·5 mg/1 free available chlorine alone. Temperature and pH did not affect the inhibitory influence of cyanuric acid on the disinfectant action of chlorine. In the swimming-pool and tap water, cyanuric acid delayed the virucidal effect of chlorine as much as in the ‚clean’ condition of chlorine-buffered distilled water. The available chlorine value should be increased to 1·5 mg/l when cyanuric acid is used in swimming-pool water.
We studied, for two years, the prevalence of indigenous human enteric viruses in wild oysters gathered each month from the bottom of Mikawa Bay, Aichi Prefecture, Japan. Viruses were detected periodically in 9 out of 54 oyster pools prepared by the acid or polyethylene glycol precipitation method although all these 9 pools met current national bacteriological safety standards. Since most of the serotypes of the enteric viruses detected in the oysters were identical with those of viruses isolated from sick children living in the area, it is suggested that contamination of enteric viruses in the oysters would depend on the prevalence of enteric viral infections in the local inhabitants.