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Target site–based penoxsulam resistance in barnyardgrass (Echinochloa crus-galli) from China

Published online by Cambridge University Press:  18 March 2019

Jiapeng Fang ,
Tingting Liu ,
Yuhua Zhang ,
Jun Li  and
Liyao Dong Open the ORCID record for Liyao Dong [Opens in a new window]
Jiapeng Fang
Affiliation:
Ph.D Candidate, College of Plant Protection, Nanjing Agricultural University, Nanjing, People’s Republic of China; State and Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing, People’s Republic of China
Tingting Liu
Affiliation:
Graduate Student, College of Plant Protection, Nanjing Agricultural University, Nanjing, People’s Republic of China; State and Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing, People’s Republic of China
Yuhua Zhang
Affiliation:
Ph.D Candidate, College of Plant Protection, Nanjing Agricultural University, Nanjing, People’s Republic of China; State and Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing, People’s Republic of China
Jun Li
Affiliation:
Associate Professor, College of Plant Protection, Nanjing Agricultural University, Nanjing, People’s Republic of China; State and Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing, People’s Republic of China
Liyao Dong*
Affiliation:
Professor, College of Plant Protection, Nanjing Agricultural University, Nanjing, People’s Republic of China; State and Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing, People’s Republic of China
*
Author for correspondence: Liyao Dong, Email: dly@njau.edu.cn
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Abstract

Barnyardgrass [Echinochloa crus-galli (L.) P. Beauv.] is acknowledged to be the most troublesome weed in rice fields in Anhui and Jiangsu provinces of China. It cannot be effectively controlled using certain acetolactate synthase (ALS)-inhibiting herbicides, including penoxsulam. Echinochloa crus-galli samples with suspected resistance to penoxsulam were collected to identify the target site–based mechanism underlying this resistance. Populations AXXZ-2 and JNRG-2 showed 33- and 7.3-fold resistance to penoxsulam, respectively, compared with the susceptible JLGY-3 population. Cross-resistance to other ALS inhibitors was reported in AXXZ-2 but not in JNRG-2, and occasionally showed higher sensitivity than JLGY-3. In vitro ALS activity assays revealed that penoxsulam concentrations required to inhibit 50% of ALS activity were 11 and 5.2 times greater in AXXZ-2 and JNRG-2, respectively, than in JLGY-3. DNA and predicted amino acid sequence analyses of ALS revealed Ala-205-Val and Ala-122-Gly substitutions in AXXZ-2 and JNRG-2, respectively. Our results indicate that these substitutions in ALS are at least partially responsible for resistance to penoxsulam.

Keywords

Patrick J. Tranel, University of IllinoisAcetolactate synthase (ALS)cross-resistancetarget-site mutant
Type
Research Article
Information
Weed Science , Volume 67 , Issue 3 , May 2019 , pp. 281 - 287
Copyright
© Weed Science Society of America, 2019 

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