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Identification of Japanese Foxtail (Alopecurus Japonicus) Resistant to Haloxyfop Using Three Different Assay Techniques

Published online by Cambridge University Press:  20 January 2017

Caihong Yang ,
Liyao Dong ,
Jun Li  and
Stephen R. Moss
Caihong Yang
Affiliation:
Key Laboratory of Monitoring and Management of Plant Disease and Insects, Ministry of Agriculture, College of Plant protection, Nanjing Agricultural University, Nanjing 210095, China
Liyao Dong*
Affiliation:
Key Laboratory of Monitoring and Management of Plant Disease and Insects, Ministry of Agriculture, College of Plant protection, Nanjing Agricultural University, Nanjing 210095, China
Jun Li
Affiliation:
Key Laboratory of Monitoring and Management of Plant Disease and Insects, Ministry of Agriculture, College of Plant protection, Nanjing Agricultural University, Nanjing 210095, China
Stephen R. Moss
Affiliation:
Plant and Invertebrate Ecology Department, Rothamsted Research, Harpenden, Hertfordshire, United Kingdom AL5 5JQ
*
Corresponding author's E-mail: dly@njau.edu.cn
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Abstract

The objective of this study was to investigate the resistance level of Japanese foxtail to haloxyfop, an acetyl coenzyme A carboxylase (ACCase; EC 6.4.1.2)–inhibiting herbicide. Eleven biotypes were collected from oilseed rape fields in different areas in Jiangsu and Anhui provinces where haloxyfop had been continuously applied for various periods. Biotypes were assessed by two different methods, a seed bioassay and whole-plant assay, to identify the most resistant and susceptible biotypes for further studies on the activity of the target enzyme ACCase. A good correlation was obtained between the two different bioassay methods. The Jurong and Chuzhou biotypes were the most resistant and susceptible biotypes, respectively, whereas the other nine biotypes showed variable and relatively low degrees of haloxyfop resistance. Furthermore, target-site enzyme sensitivity results confirmed that the Jurong biotype was resistant to haloxyfop with a concentration of herbicide causing 50% inhibition of ACCase activity (IC50) of 9.19 µM, whereas the IC50 of the susceptible biotype (Chuzhou) was 0.76 µM, giving a resistance index of 12.

Keywords

HaloxyfopJapanese foxtail, Alopecurus japonicus Steudoilseed rape, Brassica campestris L.ResistanceACCase
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 55 , Issue 6 , December 2007 , pp. 537 - 540
Copyright
Copyright © Weed Science Society of America 

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