Hostname: page-component-78c5997874-dh8gc Total loading time: 0 Render date: 2024-11-17T14:50:47.863Z Has data issue: false hasContentIssue false
  • English
  • Français

Effects of 2,4-D and Amino Acids on Field Bindweed in Vitro

Published online by Cambridge University Press:  12 June 2017

R. G. Harvey  and
T. J. Muzik
R. G. Harvey
Affiliation:
Dep. of Agron. and Soils, Washington State Univ., Pullman, WA. 99163
T. J. Muzik
Affiliation:
Dep. of Agron. and Soils, Washington State Univ., Pullman, WA. 99163
Get access Rights & Permissions [Opens in a new window]

Abstract

Two clones of field bindweed (Convolvulus arvensis L.) which differed in their susceptibility to (2,4-dichlorophenoxy)acetic acid (2,4-D) under field and greenhouse conditions also exhibited similar differences when stem cells were cultured in liquid and agar media. Amino acids added to the culture media altered the response to 2,4-D. Glutamic acid increased the tolerance of the susceptible (S) clone, but reduced the tolerance of the resistant (R) clone. Glutamine increased the susceptibility of the S clone to a much greater degree than it did the R clone. No significant differences were noted in the rates of absorption of metabolism of 2,4-D by the two clones. Glutamine increased and glutamic acid decreased 2,4-D absorption by both clones. Levels of nitrate reductase activity (NRA), soluble protein (SP), and gross RNA (GR) increased in the S tissues but decreased or remained constant in the R tissues exposed to 4.5 × 10−5M 2,4-D. Correlations between 2,4-D susceptibility and NRA demonstrated a relationship between the effects of 2,4-D and nitrogen metabolism. Differential binding of 2,4-D within the cells appears to be the most likely explanation for the differences in response to 2,4-D.

Type
Research Article
Information
Weed Science , Volume 21 , Issue 2 , March 1973 , pp. 135 - 138
Copyright
Copyright © 1973 Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

1. Andreae, W. A. and Good, N. E. 1957. Studies on 3-indoleacetic acid metabolism. IV. Conjugation with aspartic acid and ammonia as processes in the metabolism of carboxylic acids. Plant Physiol 32:566–571.CrossRefGoogle Scholar
2. Bach, M. H. and Fellig, J. 1961. Correlation between inactivation of 2,4-dichlorophenoxyacetie acid and cessation of callus growth in bean stem sections. Plant Physiol. 36: 89–91.CrossRefGoogle Scholar
3. Basler, E. and Nakazawa, K. 1961. Effects of 2,4-D on nucleic acids of cotton cotyledon tissue. Bot. Gaz. 122: 228–232.CrossRefGoogle Scholar
4. Chrispeels, M. J. and Hanson, J. B. 1962. The increase in ribonucleic acid content of cytoplasmic particles of soybean hypocotyl induced by 2,4-dichlorophenoxyacetic acid. Weeds 10:123–125.CrossRefGoogle Scholar
5. Cleland, R. 1965. Auxin-induced cell wall loosening in presence of actinomycin D. Plant Physiol. 40:595–600.CrossRefGoogle ScholarPubMed
6. Dexter, A. G., Slife, F. W., and Butler, H. S. 1971. Detoxification of 2,4-D by several plant species. Weed Sci. 19:721–726.CrossRefGoogle Scholar
7. Earle, E. 1965. Cell colony formation from isolated plant cells. Pages 401–409 in White, P. R. and Grove, A. R., eds. Proc. Int. Congr. Plant tissue culture. McCutcham Publishing Corp., Berkeley, Calif. Google Scholar
8. Fang, S. C. 1958. Absorption, translocation and metabolism of 2,4-D-1-14C in pea and tomato plants. Weeds 6:179–186.CrossRefGoogle Scholar
9. Fang, S. C. and Butts, J. S. 1954. Studies in plant metabolism. III. Absorption, translocation, and metabolism of radioactive 2,4-D in corn and wheat plants. Plant Physiol. 29:56–60.CrossRefGoogle Scholar
10. Hageman, R. H. and Flesher, Donna. 1960. Nitrate reductase activity in corn seedlings as affected by light and nitrate content of nutrient media. Plant Physiol. 35:700–708.CrossRefGoogle ScholarPubMed
11. Harvey, R. G., Muzik, T. J., and Warner, R. L. 1973. Differences in nitrogen requirements of two clones of Convolvulus arvensis in vitro. Amer. J. Bot. 60:76–79.CrossRefGoogle Scholar
12. Heimer, T. M. and Filner, P. 1971. Regulation of the nitrate assimilation pathway in cultured tobacco cells. III. The nitrate uptake system. Biochem. Biophys. Acta 230: 362–372.CrossRefGoogle Scholar
13. Jaworski, E. G. and Butts, J. S. 1952. Studies in plant metabolism. II. The metabolism of 14C-labeled 2,4-di=chlorophenoxyacetic acid in bean plants. Arch. Biochem. Biophys. 38:207–218.CrossRefGoogle Scholar
14. Lowry, O. H., Rosenbrough, N. J., Lair, A. L., and Randall, R. J. 1951. Protein measurements with the Folin phenol reagent. J. Biol. Chem. 193:265–275.CrossRefGoogle ScholarPubMed
15. Morgan, P. W. and Hall, W. C. 1963. Metabolism of 2,4-D by cotton and grain sorghum. Weeds 11:130–135.CrossRefGoogle Scholar
16. Muzik, T. J. and Lawrence, J. M. 1959. Separation of specific and nonspecific effects of chemicals on plants. Nature 183:482.CrossRefGoogle Scholar
17. Ojima, K. and Gamborg, O. L. 1968. The metabolism of 2,4-dichlorophenoxyacetic acid by suspension cultures of soybean root. Pages 857–865 in Wight, F. and Setter-field, G. (eds.). Biochemistry and physiology of plant growth substances. The Runge Press LTD., Ottawa, Canada.Google Scholar
18. Penner, D. and Ashton, F. M. 1966. Biochemical and metabolic changes in plants induced by chlorophenoxy herbicides. Residue Rev. 14:39–113.Google ScholarPubMed
19. Tulecke, W. and Nickell, L. G. 1959. Methods, problems, and results of growing plant cells under submerged conditions. New York Acad. Sci. Trans. 22:168–206.Google Scholar
20. West, S. H., Hanson, J. B., and Key, J. L. 1960. Effect of 2,4-dichlorophenoxyacetic acid on the nucleic acid and protein content of seedling tissues. Weeds 8:333–340.CrossRefGoogle Scholar
21. Whitworth, J. W. 1964. The reaction of strains of field bindweed to 2,4-D. Weeds 12:57–58.CrossRefGoogle Scholar
22. Whitworth, J. W. and Muzik, T. J. 1967. Differential response of selected clones of bindweed to 2,4-D. Weeds 21:275–280.CrossRefGoogle Scholar