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Changes in the organization of the surface membrane upon transformation of cercariae to schistosomula of the helminth parasite Schistosoma mansoni

Published online by Cambridge University Press:  06 April 2009

M. Foley ,
J. R. Kusel  and
P. B. Garland
M. Foley
Affiliation:
Department of Biochemistry, University of Dundee, Dundee DD1 4HN, Scotland
J. R. Kusel
Affiliation:
Department of Biochemistry, University of Glasgow, Glasgow G12 8QQ, Scotland
P. B. Garland
Affiliation:
Department of Biochemistry, University of Dundee, Dundee DD1 4HN, Scotland
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Summary

Merocyanin 540 (Mc540) is a fluorescent compound which is thought to bind to membranes in which there are substantial amounts of lipid in the lipid-crystalline phase. It is shown here to be of value in detecting the transformation by both mechanical and skin-penetration methods of the cercaria to the schistosomulum. The cercaria does not appear to bind Mc540, but the schistosomulum, binds Mc540 initially, in its anterior region, and at later times over the entire surface. The suggestion that transformation involves changes in the surface membrane lipid phase from gel to liquid-crystalline phase is supported by fluorescence recovery after photobleaching results with 5-N-(octadecanoyl)-amino fluorescein, a lipophilic dye which appears to be immobile in the cercaria, but fully mobile in the 40 min schistosomulum.

Type
Research Article
Information
Parasitology , Volume 96 , Issue 1 , February 1988 , pp. 85 - 97
Copyright
Copyright © Cambridge University Press 1988

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References

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