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Urinary excretion of purine derivatives in Bos indicus×Bos taurus crossbred cattle

Published online by Cambridge University Press:  08 March 2007

Álvaro Ojeda ,
Ornella de Parra ,
Joaquím Balcells  and
Álvaro Belenguer
Álvaro Ojeda
Affiliation:
Instituto de Producción Animal, Facultad de Agronomía, Universidad Central de Venezuela, Maracay Apdo. 4579, Venezuela
Ornella de Parra
Affiliation:
Instituto de Producción Animal, Facultad de Agronomía, Universidad Central de Venezuela, Maracay Apdo. 4579, Venezuela
Joaquím Balcells*
Affiliation:
Departamento de Producción Animal y Ciencia de los Alimentos, Facultad de Veterinaria, Universidad de Zaragoza, Miguel Servet 177-50 013, Spain
Álvaro Belenguer
Affiliation:
Departamento de Producción Animal y Ciencia de los Alimentos, Facultad de Veterinaria, Universidad de Zaragoza, Miguel Servet 177-50 013, Spain
*
*Corresponding author: Dr Joaquím Balcells, fax +34 976 761590, email balcells@unizar.es
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Abstract

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Four experiments were performed to study the kinetics of purine metabolism and urinary excretion in Zebu crossbred cattle. Fasting excretion was established in Expt 1, using eighteen male Bos indicus×Bos taurus crossbred cattle (261 (se 9·1) kg body weight), six of each of the following genotypes: 5/8 Bos indicus, 1/2 Bos indicus and 3/8 Bos indicus. No significant differences were observed among genotypes in fasting purine derivative excretion (277·3 (se 35·43) μmol/metabolic body weight). In a second experiment we measured the xanthine oxidase activity, which was higher in liver than in duodenal mucosa (0·64 and 0·06 (se 0·12) units/g wet tissue per min respectively; P>0·05) being in plasma 0·60 (se 0·36) units/l per min. The kinetics of uric acid were measured by intravenous pulse dose of [1,3-15N]uric acid (Expt 3). The cumulative recovery of the isotope in urine was 82 (se 6·69) %, and uric acid plasma removal, pool size and mean retention time were 0·284 (se 0·051) per h, 5·45 (se 0·823) mmol and 3·52 (se 0·521) h, respectively. Allantoin was removed from plasma at an estimated fractional rate of 0·273 (se 0·081) per h and mean retention was 3·66 (se 1·08) h. In Expt 4, the relationship between urinary purine derivative excretion (Y; mmol/d) and digestible organic matter intake (X, kg/d) was defined by the equation: Y=7·69 (se 4·2)+5·69 (se 1·68) X; n 16, Se 1·31, r 0·67.

Keywords

Bos indicusPurine derivativesXanthine oxidase
Type
Research Article
Information
British Journal of Nutrition , Volume 93 , Issue 6 , June 2005 , pp. 821 - 828
Copyright
Copyright © The Nutrition Society 2005

References

AOAC (1990) Official Methods of Analysis, 15th ed. Arlington: Association of Official Analytical Chemists.Google Scholar
Balcells, J, Guada, JA, Castrillo, C & Surra, JC (1991) Urinary excretion of allantoin precursors by sheep after differents rates of purine infusion into the duodenum. J Agric Sci (Camb) 116, 309–317.Google Scholar
Balcells, J, Guada, JAPieró, JMParker, DS (1992a) Simultaneous determination of allantoin and oxypurines in biological fluids by high-performance liquid chromatography. J Chromatogr 575, 153–157.Google Scholar
Balcells, J, Parker, DS & Seal, CJ (1992b) Purine metabolite concentration in portal and peripheral blood of steers, sheep and rats. Comp Biochem Physiol 101B, 633–636.Google Scholar
Belenguer, D, Yañez, D, Balcells, J, Ozdemir-Baber, NH, González-Ronquillo, M (2002) Urinary excretion of purine derivates and prediction of rumen microbial outflow in goats. Livest Prod Sci 77, 127–135.Google Scholar
Berlin, RD & Hawkins, RA (1968) Secretion of purines by the small intestine, general characteristics. Am J Physiol 215, 932–941.CrossRefGoogle ScholarPubMed
Chen, XB, Ørskov, ER & Hovell, FD (1990) Excretion of purine derivatives by ruminants, endogenous excretion, differences between cattle and sheep. Br J Nutr 63, 121–129.Google Scholar
Chen, XB, Kyle, DJ, Ørskov, ER & Hovell, FD (1991) Renal clearance of plasma allantoin in sheep. Exp Physiol 76, 59–65.Google Scholar
Chen, X, Samaraweera, L, Kile, D, Ørskov, ER & Abeygunawardene, H (1996) Urinary excretion of purine derivatives and tissue xanthine oxidase activity in buffaloes, with special reference to differences between buffaloes and Bos taurus. Br J Nutr 75, 397–407.Google Scholar
Daniels, ZM (1993) Purine derivatives in urine and plasma of lactating cows given different levels of feed intake. MSc Thesis, Aberdeen University.Google Scholar
Giesecke, D, Balsliemke, J, Sudekum, KH & Stangassinger, M (1993) Plasma level, clearance and renal excretion of endogenous and ruminal purines in the bovine. J Anim Physiol Anim Nutr 70, 180–189.Google Scholar
Giraldéz, FJ (1992) La excreción de catabolitos en la orina de la oveja en relación con las variaciones dietéticas en los aportes de energía y nitrógeno (Urinary excretion of catabolites in relation to dietary changes in energy and protein supply in ewes). PhD Thesis, Universidad de León.Google Scholar
González-Ronquillo, M, Balcells, J, Guada, JA & Vicente, F (2003) Purine derivative excretion in dairy cows. Endogenous excretion and effect of exogenous nucleic acid supply. J Dairy Sci 86, 1282–1291.Google Scholar
IAEA (1997) Estimation of Rumen Microbial Protein Production from Purine Derivatives in Urine. IAEA-TECDOC-945. Vienna: International Atomic Energy Agency.Google Scholar
Khan, LP & Nolan, JV (2000) Kinetics of allantoin metabolism in sheep. Br J Nutr 84, 629–634.Google Scholar
Liang, JB, Matsumoto, M, Ishida, M & Young, BA (1993) Urinary allantoin excretion in Malaysian cattle and buffaloes. In Proceeding of 7th World Conference on Animal Production, pp. 58–59 [Thomas, M, editor]. Edmonton, Canada: University of Alberta.Google Scholar
Liang, JB, Matsumoto, M & Young, BA (1994) Purine derivative excretion and ruminal microbial yield in Malaysian cattle and swamp buffalo. Anim Feed Sci Technol 47, 189–199.Google Scholar
Liang, JB, Pimpa, O, Abdullah, N, Jelan, ZA & Nolan, JV (1999) Estimation of Rumen Microbial Protein Production from Urinary Purine Derivatives in Zebu Cattle and Water Buffalo, pp. 35–42. IAEATECDOC-1093. Vienna: International Atomic Energy Agency.Google Scholar
Lindberg, JE & Murphy, M (1991) Rumen metabolism, excretion of purines derivatives and milk composition in dairy cows fed rations with negative and positive protein balance values in the rumen. In Protein Metabolism and Nutrition. Proceedings of the 6th International Symposium on Protein Metabolism and Nutrition, vol. 2, pp. 336–337 [Eggum, BO, Boisen, S, Børsting, C, Danfer, A and Hvelplund, T, editors]. Tjele, Denmark: National Institute Research.Google Scholar
Löffler, W, Gröbner, W, Medina, R & Zollner, N (1982) Influence of dietary purines on pool size, turnover and excretion of uric acid during balance conditions. Res Exp Med 181, 113–123.Google Scholar
Orellana-Boero, P, Balcells, JMartín-Orue, SMLiang, JB & Guada, JA (2001) Excretion of purine derivatives in cows, endogenous contribution and recovery of exogenous purine bases. Livest Prod Sci 68, 243–250.Google Scholar
Ørskov, E (1992) Protein Nutrition in Ruminants 2nd ed. London: Academic Press.Google Scholar
Osuji, PO, Nsahilai, IV & Khalili, H (1996) Effect of fasting on the urinary excretion of nitrogen and purine derivatives by zebu (Bos indicus) and crossbred (Bos indicus × Bos taurus) cattle. J Appl Anim Res 10, 39–47.Google Scholar
Pimpa, O, Liang, JB, Jelan, ZA & Abdullah, N (2001) Urinary excretion of duodenal purine derivatives in Kedah-Kelantan cattle. Anim Feed Sci Technol 92, 203–214.Google Scholar
Rowell, G & Walter, DE (1976) Analysing data with repeated observations on each experimental unit. J Agric Sci (Comb) 87, 423–432.Google Scholar
Soejono, M, Yusiati, LM, Budhi, SP, Widyobroto, B & Bachrudin, Z (1999) Estimating Rumen Microbial Protein Supply for Indigenous Ruminants Using Nuclear and Purine Excretion Techniques in Indonesia, pp. 43–58. IAEA-TECDOC-1093. Vienna: International Atomic Energy Agency.Google Scholar
Stangassiner, M, Chen, X, Lindberg, J & Giesecke, D (1995) Metabolism of purines in relation to microbial production. In Proceeding of the 8th International Symposium on Ruminant Physiology, pp. 387–406 [Engelhardt, WV, Leonhard-Marek, S, Breves, G and Giesecke, D, editors]. Rieder, Germany: Delmar Publishers.Google Scholar
Steel, RD & Torrie, JH (1985) Bioestadística, Principios y Procedimientos 2nd ed. Bogotá McGraw-Hill.Google Scholar
Surra, JC (1994) Excreción de derivados metabólicos de las bases púricas en la especie ovina (Urinary excretion of purine derivatives in sheep). PhD Thesis, Universidad de Zaragoza.Google Scholar
Surra, JC, Guada, JA, Balcells, J & Castrillo, C (1997) Effects of post ruminal fermentation of the fecal and urinary excretion of purines. J Anim Sci 65, 383–390.Google Scholar
Verbic, J, Chen, XB, MacLeod, NA, Ørkov, ER (1990) Excretion of purine derivatives by ruminants. Effect of microbial nucleic acid infusion on purine derivative excretion by steers. J Agric Sci (Camb) 114, 243–248.Google Scholar
Young, EG & Conway, CF (1942) On the estimation of allantoin by the Rimini-Schryver reaction. J Biol Chem 142, 839–853.Google Scholar
Zollner, N (1982) Purine and pyrimidine metabolism. Proc Nutr Soc 41, 329–342.Google Scholar