On a site in the Sudan Savanna, chemical properties of soils (except for phosphate) and the composition of cotton leaves were determined after fifteen annual treatments including dung, ammonium sulphate, single superphosphate and potassium chloride, in all combinations of three levels. In the soils dung increased C, N, cation exchange capacity, exchangeable Ca and Mg and pH, and decreased soluble Al and Mn; ammonium sulphate decreased pH, increased soluble Al and Mn, and decreased exchangeable Ca and Mg; potassium chloride had no obvious effects. In the plants dung increased P and reduced Mn; ammonium sulphate reduced Ca and Mg, and increased Mn; superphosphate increased P, Ca and Mg, and reduced K; potassium chloride increased K slightly. The most important results were the ability of ammonium sulphate to acidify the soil, as shown by soil properties and reflected in tissue composition, and the ability of dung to ameliorate these effects. Reduction of crop yield in the presence of adequate nutrient supply seems to have been due to excessive soil acidity.