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Manipulation of glutathione reductase in transgenic plants: implications for plants' responses to environmental stress

Published online by Cambridge University Press:  05 December 2011

G. P. Creissen ,
P. Broadbent ,
B. Kular ,
H. Reynolds ,
A. R. Wellburn  and
P. M. Mullineaux
G. P. Creissen
Affiliation:
Department of Applied Genetics, John Innes Institute, Colney Lane, Norwich, NR4 7UH, UK
P. Broadbent
Affiliation:
Institute of Environmental and Biological Sciences, University of Lancaster, Lancaster, LAI 4YQ, UK
B. Kular
Affiliation:
Department of Applied Genetics, John Innes Institute, Colney Lane, Norwich, NR4 7UH, UK
H. Reynolds
Affiliation:
Department of Applied Genetics, John Innes Institute, Colney Lane, Norwich, NR4 7UH, UK
A. R. Wellburn
Affiliation:
Institute of Environmental and Biological Sciences, University of Lancaster, Lancaster, LAI 4YQ, UK
P. M. Mullineaux
Affiliation:
Department of Applied Genetics, John Innes Institute, Colney Lane, Norwich, NR4 7UH, UK
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Synopsis

The activities of a number of enzymes of the ascorbate–glutathione pathway have been shown to rise under conditions of increased oxidative stress. The potential to alter the expression of specific enzymes of this pathway by genetic manipulation has provided the opportunity to attempt to develop transgenic plants with altered levels of oxidative stress defense enzymes which should have improved stress tolerances. We have cloned a cDNA for glutathione reductase from a higher plant (Pisum sativum L.) and have used this to construct chimeric genes for the expression of the pea enzyme in the chloroplast, mitochondrion or cytosol of transgenic tobacco plants. Some of the transformed lines with elevated levels of expression of glutathione reductase accumulate higher concentrations of glutathione and show increased tolerance to paraquat, however, no evidence was found for elevated tolerance to ozone fumigation.

Type
Research Article
Information
Proceedings of the Royal Society of Edinburgh, Section B: Biological Sciences , Volume 102: Oxygen and Environmental Stress in Plants , 1994 , pp. 167 - 175
Copyright
Copyright © Royal Society of Edinburgh 1994

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