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An in vitro method using bovine granulosa cells for assaying the neutralising capacity of antibodies to pregnant mare's serum gonadotrophin

Published online by Cambridge University Press:  20 November 2017

M. Kuran ,
J.S.M. Hutchinson  and
P.J. Broadbent
M. Kuran
Affiliation:
University of Aberdeen, Department of Agriculture , 581 King Street, Aberdeen, AB9 1 UD, UK Scottish Agricultural College, 581 King Street, Aberdeen, AB9 1 UD, UK
J.S.M. Hutchinson
Affiliation:
University of Aberdeen, Department of Agriculture , 581 King Street, Aberdeen, AB9 1 UD, UK
P.J. Broadbent
Affiliation:
Scottish Agricultural College, 581 King Street, Aberdeen, AB9 1 UD, UK
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Extract

Superovulatory stimulation of cattle with PMSG causes a second wave of ovarian follicular development (Saeed et al., 1989) accompanied by high concentration of oestradiol in peripheral blood (Alfuraiji et al., 1990) due to its long half life. This effect of PMSG is thought to create an unfavourable steroid environment for early embryonic development. Antibodies to PMSG (APMSG) can be used to remove PMSG from the peripheral blood of PMSG-stimulated cattle after the initial multiple follicular development and rise of oestradiol, and hence, to increases the number of transferable embryos. It has been shown that APMSG neutralises the effect of PMSG and increase the number of transferable embryos when administered relative to the preovulatory LH surge in PMSG-stimulated cattle (Alfuraiji et al., 1993). The beneficial effect of APMSG can not be realised in practice, however, because detection of the preovulatory LH surge is difficult. Therefore, using APMSG in a PMSG superovulation procedure requires further investigation to understand the mode of action of APMSG. The latter could be studied in vitro in a much more controllable way than in vivo. Furthermore, an in vitro method would allow us to test the neutralising capacity of different antibody preparations to PMSG.

Type
Ruminant Reproduction
Information
Proceedings of the British Society of Animal Science , Volume 1995 , March 1995 , pp. 138
Copyright
Copyright © The British Society of Animal Science 1995

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References

Alfuraiji, M.M., Atkinson, T., Broadbent, P.J. and Hutchinson, J.S.M. 1993. Superovulation in cattle using pmsg followed by pmsg-monoclonal antibodies. Animal Reproduction Science 33: 99–109.10.1016/0378-4320(93)90109-5CrossRefGoogle Scholar
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