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Recent Developments in Organically Doped Sol-Gel Sensors: A Microns-Scale Probe; Successful Trapping of Purified Polyclonal Antibodies; Solutions to the Dopant-Leaching Problem

Published online by Cambridge University Press:  21 February 2011

N. Aharonson ,
M. Altstein ,
G. Avidan ,
D. Avnir ,
A. Bronshtein ,
A. Lewis ,
K. Liberman ,
M. Ottolenghi ,
Y. Polevaya  and
C. Rottman
...Show all authors
N. Aharonson
Affiliation:
Institute of Plant Protection, Volcani Center, ARO, Beit Dagan 50250, Israel; and Institutes of
M. Altstein
Affiliation:
Institute of Plant Protection, Volcani Center, ARO, Beit Dagan 50250, Israel; and Institutes of
G. Avidan
Affiliation:
Applied Physics and of
D. Avnir
Affiliation:
Applied Physics and of
A. Bronshtein
Affiliation:
Institute of Plant Protection, Volcani Center, ARO, Beit Dagan 50250, Israel; and Institutes of
A. Lewis
Affiliation:
Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
K. Liberman
Affiliation:
Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
M. Ottolenghi
Affiliation:
Applied Physics and of
Y. Polevaya
Affiliation:
Applied Physics and of
C. Rottman
Affiliation:
Applied Physics and of
J. Samuel
Affiliation:
Applied Physics and of
S. Shalom
Affiliation:
Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
A. Strinkovski
Affiliation:
Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
A. Turniansky
Affiliation:
Applied Physics and of
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Abstract

We describe recent advances made in our laboratories in the general field of organically and bio-organically doped sol-gel sensors. The developments described are: (a) The first miniaturization of a sol-gel sensor down to the microns scale, with potential applications to near-field optical microscopy, using a fluorescent pH-indicator. (b) The first successful sol-gel encapsulation of purified polyclonal antibodies, and in particular an anti-nitroaromatics immunoglobulin, with which selective sensing of nitroaromatics, an important class of environmental pollutants, was demonstrated, (c) The leaching problem, occasionally encountered in doping procedures, is solved by two methodologies: First, TMOS polymerization at high acidity and low water content was found to result in non-leachable yet reactive matrices, as demonstrated with O2 sensing by excited state pyrene and with H+ sensing by excited state pyranine; and second, doping with molecules capable of forming a covalent bond within the encapsulating cage results in the permanent anchoring of the dopant. Thus, Methyl-Red, a pH indicator, was derivatized with a silylating residue, and a polymerizing TMOS was doped with it forming a pH-shifted indicator. With both methodologies, leachability was practically zero.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 346: Symposium N – Better Ceramics Through Chemistry VI , 1994 , 519
Copyright
Copyright © Materials Research Society 1998

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