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Characterization of a humic acid extracted from marine sediment and its influence on the growth of marine diatoms

Published online by Cambridge University Press:  08 April 2014

Marcela V. Martin ,
Christina Gebühr ,
Daniel O. Mártire  and
Karen H. Wiltshire
Marcela V. Martin*
Affiliation:
Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA, CONICET/UNLP), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina
Christina Gebühr
Affiliation:
Institute of Geosciences, Goethe-University Frankfurt, 60438 Frankfurt am Main, Germany and Biodiversity and Climate Research Center (BIK-F), 60325 Frankfurt am Main, Germany
Daniel O. Mártire
Affiliation:
Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA, CONICET/UNLP), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina
Karen H. Wiltshire
Affiliation:
Biologische Anstalt Helgoland, Alfred Wegener Institute for Polar and Marine Research, POB 180, 27489 Helgoland, Germany
*
Correspondence should be addressed to: M.V. Martin, Casilla de correo 16, sucursal 4 (1900), La Plata, Argentina email: mmartin@inifta.unlp.edu.ar.
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Abstract

Due to the input of humic substances from freshwater run-off into the marine habitat, the influences of such substances on marine organisms should not be neglected. We here investigate the effect of a humic acid (HA) extract from the North Sea, characterized by spectroscopic techniques and carboxylic and phenolic group content, on the growth of different algae. Two benthic pennate diatoms isolated from the Baltic Sea, Navicula ramosissima (C. Agardh) Cleve, 1895 and Entomoneis paludosa (W. Smith) Reimer, 1975, as well as two tychopelagic centric diatoms isolated from the North Sea, Melosira nummuloides C. Agardh, 1824 and Paralia sulcata (Ehrenberg) Cleve, 1873, were employed. The concentrations of pigments (fucoxanthin, diadinoxanthin, chlorophyll-a and β-carotene) and nutrients were also measured. Adding low concentrations of naturally humic substances (5.5 mg of C l−1) to the algae cultures resulted in enhanced growth rates compared to the control experiments, possibly due to the increase in the bioavailability of trace metals or other nutrients.

Keywords

Helgoland Roadshumic substancesmarine diatomsNorth SeaParalia sulcatagrowth experiments
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 94 , Issue 5 , August 2014 , pp. 895 - 906
Copyright
Copyright © Marine Biological Association of the United Kingdom 2014 

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