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Glutathione-s-transferase is an important antigen in the eel nematode Anguillicola crassus

Published online by Cambridge University Press:  05 June 2009

M.E. Nielsen  and
K. Buchmann
M.E. Nielsen
Affiliation:
Marine Biological Laboratory, University of Copenhagen, 5 Strandpromenaden, DK-3000 Helsingør, Denmark:
K. Buchmann
Affiliation:
Section of Fish Diseases, Department of Veterinary Microbiology, Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1870 Frederiksberg C, Denmark
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Abstract

Different organs and secretions/excretions of the swimbladder parasite, Anguillicola crassus (Nematoda), were tested for the presence of antigens to the humoral immune response previously detected in the European eel, Anguilla anguilla. Proteins from different fractions of Anguillicola crassus were separated using SDS–PAGE (sodium-dodecyl-sulphate polyacrylamide-gel electrophoresis) under reducing conditions and electroblotted onto nitrocellulose membranes. Infected eels showed a specific antibody response to a 43 kDa antigen in the cuticle and towards two gonad antigens around 34 and 43 kDa. In protein released from the worms, two secretory/excretory antigens of approximately 28 kDa were found. The secretion/excretion rate of protein from the parasite to the surroundings was determined. Subsequently, an ELISA system was established applying these antigens as the first layer of coating. Furthermore, antigens from Anguillicola crassus were examined for the presence of glutathione-s-transferase (GST) using a specific antibody against GST. The antigens were found to be subunits of GST.

Type
Research Papers
Information
Journal of Helminthology , Volume 71 , Issue 4 , December 1997 , pp. 319 - 324
Copyright
Copyright © Cambridge University Press 1997

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References

Buchmann, K., Pedersen, L.Ø. & Glamann, J. (1991) Humoral immune response of European eel Anguilla anguilla to a major antigen in Anguillicola crassus (Nematoda). Diseases of Aquatic Organisms 12, 55–57.CrossRefGoogle Scholar
Buchmann, K., Pedersen, L.Ø. & Glamann, J. (1992) Affinity purification of antigen-specific serum immunoglobulin from the European eel (Anguilla anguilla). Scandinavian Journal of Immunology 36, 89–97.Google Scholar
Canals, A. & Gasbarre, L.C. (1990) Ostertagia ostertagi: isolation and partial characterisation of somatic and metabolic antigens. International Journal for Parasitology 20, 1047–1054.Google Scholar
DeCharleroy, D., Cannaerts, V., Augustijn, H., Grisez, L., Boon, J.H. & Ollevier, F. (1990) An improved method for artificial infection of the European eel, Anguilla anguilla, with Anguillicola crassus (Nematoda, Dracunculoidea). Journal of Applied Ichthyology 6, 182–188.Google Scholar
Gamble, H.R. & Graham, C.E. (1984) Monoclonal antibody-purified antigen for the immunodiagnosis of trichinosis. American Journal of Veterinary Research 45, 67–74.Google Scholar
Gottstein, B. & Felleisen, R. (1995) Protective immune mechanisms against the metacestode of Echinococcus multilocularis. Parasitology Today 11, 320–326.Google Scholar
Habig, W.H., Pabst, M.J. & Jakoby, W.B. (1974) Glutathione S-transferases. Journal of Biological Chemistry 249, 7130–7139.Google Scholar
Haenen, O.L.M., Grisez, L., DeCharleroy, D., Belpaire, C. & Ollevier, F. (1989) Experimentally induced infections of European eel (Anguilla anguilla) with Anguillicola crassus (Nematoda, Dracunculoidea) and subsequent migration of larvae. Diseases of Aquatic Organisms 7, 97–101.Google Scholar
Haenen, O.L.M., Van Wijngaarden, T.A.M., Van der Heijden, M.H.T., Höglund, J., Cornelissen, J.B.J.W., Van Leengoed, L.A.M., Borgsteede, F.H.M. & Van Muswinkel, W.B. (1996) Effects of experimental infections with different doses of Anguillicola crassus (Nematoda, Dracunculoidea) on European eel (Anguilla anguilla). Aquaculture 141, 41–57.Google Scholar
Höglund, J. & Pilström, L. (1994) Purification of adult Anguillicola crassus whole-worm extract antigens for detection of specific antibodies in serum from the European eel (Anguilla anguilla) by ELISA. Fish and Shellfish Immunology 4, 311–319.Google Scholar
Höglund, J. & Pilström, L. (1995) Mechanical isolation and characterization of antigens from adult Anguillicola crassus. Fish and Shellfish Immunology 5, 51–60.Google Scholar
Ketterer, B., Meyer, D.J. & Clark, A.G. (1988) Soluble glutathione transferase isoenzymes. pp. 73–135in Sies, H. & Ketterer, B.Glutathione conjugation, its mechanism and biological significance. London, Academic Press.Google Scholar
Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.Google Scholar
Lobos, E., Weiss, N., Karam, M., Taylor, H.R., Ottesen, E.A. & Nutman, T.B. (1991) An immunogenic Onchocerca volvulus antigen: a specific and early marker of infection. Science 25, 1603–1605.Google Scholar
Molnár, K., Baska, F., Csaba, G., Gláavits, R. & Székely, C. (1993) Pathological and histopathological studies of the swimbladder of eels Anguilla anguilla infected by Anguillicola crassus (Nematoda: Dracunculoidea). Diseases of Aquatic Organisms 15,41–50.CrossRefGoogle Scholar
Sharp, P.J., Smith, D.R.J., Bach, W., Wagland, B.M. & Cobon, G.S. (1991) Purified glutathione S-transferase from parasites as candidate protective antigens. International Journal of Parasitology 21, 839–846.Google Scholar
Smith, D.B., Davern, K.M., Board, P.G., Tiu, W.U., Garcia, E.G. & Mitchell, G.F. (1986) The Mr 26,000 antigen of Schistosoma japonica recognized by resistant WEHI 129/J mice is a parasite glutatione s-transferase. Proceedings of the National Academy of Sciences, USA 83, 9703–8707.Google Scholar
Voller, A. & Savigny, D. (1981) Diagnostic serology of tropical diseases. Journal of Immunological Methods 46,1–29.Google Scholar