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Effects of 5-hydroxytryptamine (serotonin) on the incorporation of 32P-inorganic phosphate into phospholipids in Hymenolepis diminuta (Cestoda)

Published online by Cambridge University Press:  05 June 2009

Y. K. Ip  and
M. M. Khan
Y. K. Ip
Affiliation:
Parasitology Laboratory, Department of Zoology, National University of Singapore, Kent Ridge, Singapore 0511
M. M. Khan
Affiliation:
Parasitology Laboratory, Department of Zoology, National University of Singapore, Kent Ridge, Singapore 0511
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Abstract

5-Hydroxytryptamine (5-HT) (1, 2 and 5 mM) significantly stimulated the incorporation of radioactive inorganic phosphate (32Pi) into phosphatidylinositol, phosphatidyiethanolamine, phosphatidic acid and also total phospholipid fraction of Hymenolepis diminuta after one hour of incubation. Such effect was both time and concentration dependent. In the presence of 5-HT early labelling of phosphatidylinositol was observed. Also, the percentage stimulation by 5-HT was the highest in this fraction under all experimental conditions. The inorganic, organic, total and phosphatidylcholine-bound phosphate of H. diminuta incubated with 5-HT were not significantly different from those of the control under all incubation conditions. Results reported herein suggest that messenger molecules that are derived from phosphoinositides may be involved in the stimulatory mechanism of 5-HT in H. diminuta.

Keywords

Hymenolepis diminuta5-hydroxytryptaminephosphatephospholipidsphosphoinositides
Type
Research Article
Information
Journal of Helminthology , Volume 64 , Issue 3 , September 1990 , pp. 203 - 211
Copyright
Copyright © Cambridge University Press 1990

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