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The kinetics of the precipitation of chemically modified αs1-casein by calcium

Published online by Cambridge University Press:  01 June 2009

David S. Horne
David S. Horne
Affiliation:
Hannah Research Institute, Ayr, Scotland, KA6 5HL
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Summary

The aggregation of αs1-casein by Ca is viewed as resulting from a reduction in the net negative charge by the binding of positively charged Ca2+. This reduction in charge leads to the equivalent of isoelectric precipitation. A quantitative relationship between the rate of aggregation of the casein and the monomer net charge is predicted in this isoelectric precipitation model. Precipitation results are presented for αs1-casein labelled with dansyl chloride. Providing the changes in charge brought about by the modification are taken into account, the precipitation behaviour of these caseins can be reconciled with that of native αs1-casein. This emphasises the role of electrostatic repulsion in this process and lends further support to the isoelectric precipitation model.

Type
Section C. Association of Proteins
Information
Journal of Dairy Research , Volume 46 , Issue 2: Symposium on the Physics and Chemistry of Milk Proteins , April 1979 , pp. 265 - 269
Copyright
Copyright © Proprietors of Journal of Dairy Research 1979

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References

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