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Effects of intravenous triacylglycerol emulsions on lymphocyte responses to mitogens in fasted dairy cows undergoing intense lipomobilization

Published online by Cambridge University Press:  30 April 2007

Nicola Lacetera ,
Daniela Scalia ,
Dough G Mashek ,
Umberto Bernabucci  and
Ric R Grummer
Nicola Lacetera*
Affiliation:
Dipartimento di Produzioni Animali, Università della Tuscia, Italy
Daniela Scalia
Affiliation:
Dipartimento di Produzioni Animali, Università della Tuscia, Italy
Dough G Mashek
Affiliation:
Department of Dairy Science, University of Wisconsin-Madison, USA
Umberto Bernabucci
Affiliation:
Dipartimento di Produzioni Animali, Università della Tuscia, Italy
Ric R Grummer
Affiliation:
Department of Dairy Science, University of Wisconsin-Madison, USA
*
*For correspondence; e-mail: nicgio@unitus.it
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Abstract

The objective of the study was to assess the effects of intravenous infusion of triacylglycerol (TAG) emulsions derived from different lipid sources on responses to mitogens of peripheral blood mononuclear cells (PBMC) isolated from fasted dairy cows. Six multiparous, non-pregnant, non-lactating Holstein cows were used in a replicated 3×3 Latin Square design. For 4 d, cows were fasted and infused intravenously with a 20% TAG emulsions derived from tallow (TA), linseed oil (LO) or fish oil (FO). Fasting was employed to induce energy deficit and lipid mobilization. Emulsions were administered for 20 to 30 min every 4 h throughout the 4 d fast at a rate of 0·54 g TAG/kg BW/d. Blood samples were taken before the first infusion, and then every 24 h during the fast. Blood was utilized to assess DNA synthesis, IgM and interferon-gamma (IFN-γ) secretion by PBMC stimulated with mitogens. In TA infused cows there was a decline of PBMC ability to respond to mitogens, which was significant 48 h after initiation of the infusion period for DNA synthesis and IFN-γ secretion. In LO or FO infused cows, PBMC responses to mitogens were not altered during the infusion period, and in some cases PBMC responses to mitogen was improved at 72 and 96 h after initiation of treatments. Effects of TAG infusion on PBMC responses to mitogens depended on the lipid source suggesting that LO or FO can attenuate the negative effects of fasting on immune functions.

Keywords

Triacylglycerol emulsionsdairy cowslymphocytes
Type
Research Article
Information
Journal of Dairy Research , Volume 74 , Issue 3 , August 2007 , pp. 323 - 328
Copyright
Copyright © Proprietors of Journal of Dairy Research 2007

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