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A deleted hobo element is involved in the unstable thermosensitive vgal mutation at the vestigial locus in Drosophila melanogaster

Published online by Cambridge University Press:  14 April 2009

C. Bazin ,
J. Williams ,
J. Bell  and
J. Silber
C. Bazin*
Affiliation:
Université Paris 7, Institut J. Monod, LGQM Tour 42–32 5éme étage, 2 place Jussieu, 75005 Paris, France
J. Williams
Affiliation:
Howard Hughes Medical Institute, Laboratory of Molecular Biology, University of Wisconsin-Madison, Madison, Wisconsin 53700USA
J. Bell
Affiliation:
Department of Genetics, University of Alberta, Edmonton, Alberta, Canada T6G-2E9
J. Silber
Affiliation:
Université Paris 7, Institut J. Monod, LGQM Tour 42–32 5éme étage, 2 place Jussieu, 75005 Paris, France
*
*Corresponding author, CNRS, laboratoire de Biologie et Génétiques Evolutives, 91198 Gif-sur-Yvette Cedex, France.
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We have described a new unstable mutant of the vestigial locus isolated from a natural population. From this mutant, vestigialalmost (vgal), wild-type (vgal+), and extreme (vgext), alleles arose spontaneously. The molecular analysis of vgal shows that the mutation is due to a 1874 bp hobo element inserted in a vestigial intron. Two distinct kinds of events lead a wild-type phenotype. Three independent vgal+ alleles result from an excision of the hobo element and two other vgal+ alleles have further deletions of hobo sequence. The sequence of one of them shows a 1516 bp hobo insertion at the same place and in the same orientation as the 1874 bp insertion. In the vgext alleles, we found a 5′ or 3′ variably sized deletion of vg sequences. One of them, which has been cloned and sequenced, has a deletion finishing exactly at the left terminal repeat′ hobo element. The genetic implications of these different genetic structures are discussed.

Type
Research Article
Information
Genetics Research , Volume 61 , Issue 3 , June 1993 , pp. 171 - 176
Copyright
Copyright © Cambridge University Press 1993

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