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The infectivity of chorio-allantoic membrane influenza virus and incomplete influenza virus by the six-hour soluble antigen productionl test

Published online by Cambridge University Press:  15 May 2009

A. J. Beale  and
N. B. Finter
A. J. Beale
Affiliation:
Public Health Laboratory, Northampton
N. B. Finter
Affiliation:
Public Health Laboratory, Northampton
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1. After inoculation of eggs with a large dose (108·6 ID50) of influenza virus, chorio-allantoic membranes harvested from the 3rd to at least the 21st hr. (but not at the 1st or 2nd hr.) gave rise to large amounts of soluble antigen in 6 hr. in the chorio-allantoic membranes of sub-inoculated eggs (6 hr. soluble antigen production test). These amounts were larger than resulted from inoculation of equivalent numbers of ID50 of standard allantoic fluid virus. By the conventional 72 hr. test, the infectivity of the chorio-allantoic membranes increased 1 hr. later, at the 4th hr. after inoculation.

2. Allantoic fluids and chorio-allantoic membranes harvested from passages of very large and of small amounts of influenza virus were tested by 6 hr. soluble antigen production. The relative amounts of soluble antigen formed by different seeds appeared to be determined by their ID50: HA ratios, irrespective of whether they were allantoic fluid or chorio-allantoic membrane preparations. Larger amounts of soluble antigen were formed in 6 hr. per ID50 inoculated by preparations having lower ID50: HA ratios, i.e. containing larger amounts of ‘incomplete’ virus, and vice versa.

3. It is suggested that high infectivity in the 6 hr. soluble antigen test per ID50 inoculated is due to a content of ‘incomplete’ virus, or of virus precursors: possibly these are in fact the same.

4. More particles of an incomplete virus preparation than of standard allantoic fluid virus must be inoculated to produce a given amount of soluble antigen in 6 hr. It is suggested that a process resembling multiplicity reactivation must occur in cells infected with more than one particle of ‘incomplete’ virus.

Type
Research Article
Information
Journal of Hygiene , Volume 54 , Issue 1 , March 1956 , pp. 68 - 78
Copyright
Copyright © Cambridge University Press 1956

References

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