Hostname: page-component-78c5997874-s2hrs Total loading time: 0 Render date: 2024-11-18T08:37:39.406Z Has data issue: false hasContentIssue false
  • English
  • Français

Enhanced detection of Candida in blood cultures with the BACTEC 460 system by use of the aerobic-hypertonic (8B) medium

Published online by Cambridge University Press:  15 May 2009

P. Yagupsky ,
F. S. Nolte  and
M. A. Menegus
P. Yagupsky
Affiliation:
Clinical Microbiology Laboratories, University of Rochester Medical Center, 601 Elmwood Avenue, Box 710, Rochester, New York 14642, USA
F. S. Nolte
Affiliation:
Clinical Microbiology Laboratories, University of Rochester Medical Center, 601 Elmwood Avenue, Box 710, Rochester, New York 14642, USA
M. A. Menegus
Affiliation:
Clinical Microbiology Laboratories, University of Rochester Medical Center, 601 Elmwood Avenue, Box 710, Rochester, New York 14642, USA
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The blood culture records during the 6-year period 1984–9 were reviewed to compare the performance of the BACTEC aerobic (6B) and aerobic-hypertonic (8B) media for the recovery of Candida spp. and Torulopsis glabrata from blood. There were 137 positive blood culture sets that contained both a 6B and an 8B bottle. Sixty-eight different yeasts were recovered from 65 patients including 35 Candida albicans, 19 C. tropicalis, 6 C. parapsilosis, 4 C. krusei, 1 C. pseudotropicalis and 3 Torulopsis glabrata. The 8B medium detected 120 of the positive cultures (87·6%) and was the only positive medium in 35 (25·6%) sets, while the 6B medium detected 102 positive cultures (74·4%) and was the only positive medium in 17 (12·4%) sets (P < 0·04). For those sets in which both bottles were positive, radiometric detection occurred first in the 8B bottle in 39 sets and first in the 6B bottle in 11 sets (P < 0·001). The superior performance of the 8B bottle was not related to the administration of amphotericin B. Cultures of stock strains of C. albicans, C. tropicalis, and C. parapsilosis in 6B and 8B media with and without added blood confirmed the finding that 8B was substantially superior to 6B for the detection of candidaemia. It is concluded that an 8B bottle should be included in the blood culture set whenever candidaemia is suspected.

Type
Research Article
Information
Epidemiology & Infection , Volume 105 , Issue 3 , December 1990 , pp. 553 - 558
Copyright
Copyright © Cambridge University Press 1990

References

REFERENCES

1.Repentigny, L, Reiss, E. Current trends in immunodiagnosis of oandidiasis and aspergillosis. Rev Infect Dis 1984; 6: 301–12.Google Scholar
2.Fung, JC, Donta, ST, Tilton, RC. Candida Detection System (CAND-TEC) to differentiate between Candida albicans colonization and disease. J Clin Microbiol 1986; 24: 542–7.CrossRefGoogle ScholarPubMed
3.Myerowitz, RL, Pazin, GJ, Allen, DM. Disseminated candidiasis: changes in incidence, underlying diseases and pathology. Am J Clin Pathol 1977; 68: 29–38.CrossRefGoogle ScholarPubMed
4.Eng, J, Maeland, A. Correlation of growth of aerobic blood cultures in hypertonic broth with antibiotic therapy. J Clin Microbiol 1982; 16: 890–4.CrossRefGoogle ScholarPubMed
5.Henrichsen, J, Bruun, B. An evaluation of the effects of a high concentration of sucrose in blood culture media. Acta Path Microbiol Scand 1973; 81: 707–10.Google Scholar
6.LaScolea, LJ, Sullivan, TD, Dryja, D, Meter, E, 1983. Advantages of BACTEC hypertonic culture medium for detection of Haemophilus influenzae bacteremia in children. J Clin Microbiol 1983; 17: 1177–9.CrossRefGoogle Scholar
7.Rosner, R. Comparison of isotonic and radiometric-hypertonic cultures for the recovery of organisms for cerebrospinal, pleural and synovial fluids. Am J Clin Pathol 1975; 63: 149–52.CrossRefGoogle ScholarPubMed
8.Cooper, B, Silva-Hunter, M. Yeasts of medical importance. In: Lennette, EH, Balows, A, Hausler, WJ, Shadomy, HJ, eds. Manual of clinical microbiology. Washington DC: American Society for Microbiology, 1985: 526–41.Google Scholar
9.Reimer, LG, McDaniel, JD, Mirrett, S, Reller, LB, Wang, WL. Controlled evaluation of supplemented peptone and BACTEC blood culture broths for the detection of bacteremia and fungemia. J Clin Microbiol 1985; 21: 531–4.CrossRefGoogle ScholarPubMed
10.Prevost, E, Bannister, E. Detection of yeast septicemia by biphasic and radiometric methods. J Clin Microbiol 1976; 4: 216–24.Google Scholar
11.Brannon, P, Kiehn, TE. Large-scale clinical comparison of the lysis-centrifugation and radiometric systems for blood culture. J Clin Microbiol 1985; 22: 951–4.Google Scholar
12.Ellner, PD, Kiehn, TE, Beebe, JL, McCarthy, LR. Critical analysis of hypertonic medium and agitation in detection of bacteremia. J Clin Microbiol 1976; 4: 216–24.CrossRefGoogle ScholarPubMed
13.Coleman, RM, Laslie, WW, Lambe, DW. Clinical comparison of aerobic, hypertonic and anaerobic culture media for the radiometric detection of bacteremia. J Clin Microbiol 1876; 3: 281–6.Google Scholar
14.Crist, AE, Amsterdam, D, Meter, E. Superiority of hypertonic culture medium for detection of Haemophilus influenzae by the BACTEC procedure. J Clin Microbiol 1982; 15: 228–30.Google Scholar
15.Rosner, R. Isolation of Candida protoplasts from a case of Candida endocarditis. J Bacteriol 1966; 91: 1320–6.CrossRefGoogle ScholarPubMed
16.Jungkind, D, Millan, J, Allen, S, Dyke, J, Hill, E. Clinical comparison of a new automated infrared blood culture system with the BACTEC 460 system. J Clin Microbiol 1986; 23: 262–6.CrossRefGoogle ScholarPubMed