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The direct fluorescent antibody test for detection of Brucella abortus in bovine abortion material

Published online by Cambridge University Press:  15 May 2009

M. J. Corbel
Affiliation:
Ministry of Agriculture, Fisheries and Food, Central Veterinary Laboratory, Weybridge, Surrey
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Summary

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The direct fluorescent antibody test was assessed for detection of Brucella abortus in bovine abortion material. Br. abortus organisms could be readily detected as characteristic intra-cellular clumps, in smears stained with fluorescent antibody. Counterstaining with Evans Blue was necessary to suppress tissue auto-fluorescence. The method was specific and enabled brucella infection to be differentiated, inter alia, from Q-fever infection.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1973

References

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