Hostname: page-component-78c5997874-94fs2 Total loading time: 0 Render date: 2024-11-18T16:42:16.120Z Has data issue: false hasContentIssue false
  • English
  • Français

A comparison of the R25 modification of Rappaport's enrichment medium with strontium chloride B for salmonella isolation from sewage polluted natural water

Published online by Cambridge University Press:  25 March 2010

R. W. S. Harvey  and
T. H. Price
R. W. S. Harvey
Affiliation:
Regional Public Health Laboratory, University Hospital of Wales, Heath Park, Cardiff CF4 4XW
T. H. Price
Affiliation:
Regional Public Health Laboratory, University Hospital of Wales, Heath Park, Cardiff CF4 4XW
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The relation of salmonella isolation efficiency and the size of inoculum introduced from a buffered peptone water culture of sewage polluted water into strontium chloride B medium was investigated. Two separate studies were made, one using enrichment at 37°C, the other at 43°C. From these trials, two inocula suitable for efficient salmonella isolation were determined. Using this information, strontium chloride B medium was compared with modified Rappaport's broth (R25). The inoculum used with R25 was 0·005 ml, determined in an earlier study. Two incubation temperatures were employed with strontium chloride enrichment (37 and 43°C). Rappaport's medium was incubated at 37°C only. Elevated temperature enrichment at 43°C improved the performance of strontium chloride B, but Rappaport's broth still gave significantly better results. This supports earlier studies on simplification of salmonella isolation and standardization of routine technique on a single enrichment medium: Rappaport broth (R25) incubated at 37°C.

Type
Research Article
Information
Journal of Hygiene , Volume 89 , Issue 1 , August 1982 , pp. 111 - 117
Copyright
Copyright © Cambridge University Press 1982

References

REFERENCES

Anon, (1975). Meat and meat products – Detection of Salmonellae (reference method) International Standard ISO 3565 – 1975(E) Geneva: International Organization for Standardization.Google Scholar
Eisenberg, P. (1919). Untersuchungen über spezifische Desinfektionsvorgänge. II Mitteilung. Ueber die Wirkung von Salzen und Ionen auf Bakterien. Zentralblatt für Bakteriologie, Parasitenkunde und Infektionskrankheiten. I. Abteilung Originale 82, 69.Google Scholar
Guth, F. (1916). Selennährböden für die elektive züchtung von typhusbacillen. Zentralblatt für Bakteriologie, Parasitenkunde und Infektionskrankheiten. I. Abteilung Originale 77, 487.Google Scholar
Harvey, R. W. S. (1956). Choice of a selective medium for the routine isolation of members of the salmonella group. Monthly Bulletin of the Ministry of Health and the Public Health Laboratory Service 15, 118.Google Scholar
Harvey, R. W. S. & Price, T. H. (1974). Isolation of Salmonellas. Public Health Laboratory Service Monograph Series, 8, London: HMSO.Google Scholar
Harvey, R. W. S. & Price, T. H. (1980). Salmonella isolation with Rappaport's medium after pre-enrichment in buffered peptone water using a series of inoculum ratios. Journal of Hygiene 85, 125.CrossRefGoogle ScholarPubMed
Harvey, R. W. S. & Price, T. H. (1981). Comparison of selenite F, Muller-Kauffmann tetrathionate and Rappaport's medium for Salmonella isolation from chicken giblets after pre-enrichment in buffered peptone water. Journal of Hygiene 87, 219.Google Scholar
Harvey, R. W. S., Price, T. H. & Crone, P. B. (1975). Quality control tests of two salmonella enrichment media using different inocula. Journal of Hygiene 74, 375.CrossRefGoogle ScholarPubMed
Harvey, R. W. S. & Thomson, S. (1953). Optimum temperature of incubation for isolation of salmonellae. Monthly Bulletin of the Ministry of Health and the Public Health Laboratory Service 12, 149.Google Scholar
Harvey, R. W. S., Price, T. H. & Xirouchaki, E. (1979). Comparison of selenite F, Muller-Kauffmann tetrathionate and Rappaport's medium for the isolation of salmonellas from sewage-polluted natural water using a pre-enrichment technique. Journal of Hygiene 83, 451.CrossRefGoogle ScholarPubMed
Hotchkiss, M. (1923). Studies on salt action. VI. The stimulatory and inhibitive effect of certain cations upon bacterial growth. Journal of Bacteriology 8, 141.Google Scholar
Iveson, J. B. (1971). Strontium chloride B and EE enrichment broth media for the isolation of Edwardsiella, Salmonella and Arizona species from tiger snakes. Journal of Hygiene 69, 323.CrossRefGoogle ScholarPubMed
Iveson, J. B. & Mackay-Scollay, E. M. (1969). Strontium chloride and strontium selenite enrichment media in the isolation of Salmonella. Journal of Hygiene 67, 457.CrossRefGoogle ScholarPubMed
Iveson, J. B. & Mackay-Scollay, E. M. (1972). An evaluation of strontium chloride, Rappaport and strontium selenite enrichment for the isolation of salmonellas from man, animals, meat products and abattoir effluents. Journal of Hygiene 70, 367.Google Scholar
Jameson, J. E. (1963). A note on the isolation of salmonellae. Journal of Applied Bacteriology 26, 112.CrossRefGoogle Scholar
Kauffmann, F. (1930). Ein kombiniertes Anreicherungsverfahren für Typhus-und Paratyphusbazillen. Zentralblatt für Bakteriologie. Parasitenkunde und Infektionskrankheiten. I. Abteilung Originale 119, 148.Google Scholar
Kauffmann, F. (1935). Weitere Erfahrungen mit dem kombinierten Anreicherungsverfahren für Salmonella bazillen. Zeitschrift für Hygiene und Infektionskrankheiten 117, 26.Google Scholar
Leifson, E. (1936). New selenite enrichment media for the isolation of typhoid and paratyphoid (salmonella) bacilli. American Journal of Hygiene 24, 423.Google Scholar
Muller, L. (1923). Un nouveau milieu d'enrichissement pour la recherche du bacille typhique et des paratyphiques. Comptes Rendus des Séances de la Société de Biologie et de ses Filiales 89, 434.Google Scholar
Rappaport, F. & Konforti, N. (1959). Selective enrichment medium for paratyphoid bacteria. Inhibitory and growth promoting factors. Applied Microbiology 7, 63.CrossRefGoogle ScholarPubMed
Rappaport, F., Konforti, N. & Navon, B. (1956). A new enrichment medium for certain salmonellae. Journal of Clinical Pathology 9, 261.Google Scholar
Sen, R. (1964). An assessment of the relative efficiency of several media in the isolation of Shigellae and Salmonellae from faeces. Indian Journal of Pathology and Bacteriology 7, 93–101.Google Scholar
Vassiliadis, P., Trichopoulos, D., Kalandidi, A. & Xirouchaki, E. (1978). Isolation of salmonellae from sewage with a new procedure of enrichment. Journal of Applied Bacteriology 44, 233.Google Scholar
Vassiliadis, P., Patéraki, E., Papaïconomou, N., Papadakis, J. A. & Trichopoulos, D. (1976). Nouveau procédé d'enrichissement de Salmonella. Annales de Microbiologie (Institut Pasteur) 127 B, 195.Google Scholar
Vassiliadis, P., Trichopoulos, D., Papadakis, J. & Politi, G. (1970). Salmonella isolations in abattoirs in Greece. Journal of Hygiene 68, 601.Google ScholarPubMed