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3 - In vitro models for studying pre-eclampsia

from Part I - Basic science

Published online by Cambridge University Press:  03 September 2009

By
John D. Aplin  and
Ian P. Crocker
Edited by
Fiona Lyall  and
Michael Belfort
Fiona Lyall
Affiliation:
University of Glasgow
Michael Belfort
Affiliation:
University of Utah
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Summary

Introduction

Profound morphological changes occur during the comparatively short life span of the placenta (Benirschke and Kaufmann, 2000; Fox, 1997). Most of these can be related directly to functional requirements; establishing support for fetal development and growth, maintaining an immunological barrier and adjusting maternal physiology to meet the demands of pregnancy. Histology and ultrastructure present snapshots of cell and tissue behavior, but not an account of cellular interactions or pathological mechanisms. Appropriate and robust in vitro models are therefore essential in bridging the gap between structure and function, as they can accommodate mechanistic questions and offer scope for the design and testing of possible therapeutic interventions. Models should mimic cell responses in vivo and go at least part way to reflecting physiological events within the placenta. Experimental levels range from tissue perfusion to explant and cell culture. Recently, genomic, transcriptomic, proteomic and computational biology approaches have become available to complement and extend in vitro methodologies. To appreciate how these models have been applied to studying pre-eclampsia, and the scope of the in vitro methods currently available, it is convenient to divide the placenta into structurally and functionally distinct compartments, i.e. the chorionic villus and the placental bed. In turn, we have subdivided these into individual cellular components, namely those of the trophoblast, vasculature and stroma.

Villus components

Cytotrophoblast and syncytiotrophoblast in primary culture

Cytotrophoblast cells of the human placenta are the precursors of all other trophoblast phenotypes. As such, a variety of methods have been employed for their purification.

Type
Chapter
Information
Pre-eclampsia
Etiology and Clinical Practice
 , pp. 37 - 49
Publisher: Cambridge University Press
Print publication year: 2007

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