Laser-induced forward transfer (LIFT) is a nozzle-free printing technology that can be used for two- and three-dimensional printing. In LIFT, a laser pulse creates an impulse inside a thin film of material that results in the formation of a liquid jet. We experimentally study LIFT of viscoplastic materials by visualizing the process of jetting with high-speed imaging. The shape of the jet depends on the laser energy, focal height, surface tension and material rheology. We theoretically identify the characteristic jetting velocity and how it depends on the control parameters, and define non-dimensional groups to classify the regimes of jetting. Based on the results, we propose the optimal conditions for printing with LIFT technology.

Very Long Baseline Interferometry (VLBI) at sub-millimeter waves has the potential to image the shadow of the black hole in the Galactic Center, Sagittarius A* (Sgr A*), and thereby test basic predictions of the theory of general relativity. We investigate the imaging prospects of a new Space VLBI mission concept. The setup consists of two satellites in polar or equatorial circular Medium-Earth Orbits with slightly different radii, resulting in a dense spiral-shaped uv-coverage with long baselines, allowing for extremely high-resolution and high-fidelity imaging of radio sources. We simulate observations of a general relativistic magnetohydrodynamics model of Sgr A* for this configuration with noise calculated from model system parameters. After gridding the uv-plane and averaging visibilities accumulated over multiple months of integration, images of Sgr A* with a resolution of up to 4 μ as could be reconstructed, allowing for stronger tests of general relativity and accretion models than with ground-based VLBI.

Excessive salt intake is a common feature of Western dietary patterns, and has been associated with important metabolic changes including cerebral redox state imbalance. Considering that little is known about the effect on progeny of excessive salt intake during pregnancy, the present study investigated the effect of a high-salt diet during pregnancy and lactation on mitochondrial parameters and the redox state of the brains of resulting offspring. Adult female Wistar rats were divided into two dietary groups (n 20 rats/group): control standard chow (0·675 % NaCl) or high-salt chow (7·2 % NaCl), received throughout pregnancy and for 7 d after delivery. On postnatal day 7, the pups were euthanised and their cerebellum, hypothalamus, hippocampus, prefrontal and parietal cortices were dissected. Maternal high-salt diet reduced cerebellar mitochondrial mass and membrane potential, promoted an increase in reactive oxygen species allied to superoxide dismutase activation and decreased offspring cerebellar nitric oxide levels. A significant increase in hypothalamic nitric oxide levels and mitochondrial superoxide in the hippocampus and prefrontal cortex was observed in the maternal high-salt group. Antioxidant enzymes were differentially modulated by oxidant increases in each brain area studied. Taken together, our results suggest that a maternal high-salt diet during pregnancy and lactation programmes the brain metabolism of offspring, favouring impaired mitochondrial function and promoting an oxidative environment; this highlights the adverse effect of high-salt intake in the health state of the offspring.

When a liquid drop is placed on a highly superheated surface, it can be levitated by its own vapour. This remarkable phenomenon is referred to as the Leidenfrost effect. The thermally insulating vapour film results in a severe reduction of the heat transfer rate compared to experiments at lower surface temperatures, where the drop is in direct contact with the solid surface. A commonly made assumption is that this solid surface is isothermal, which is at least questionable for materials of low thermal conductivity, resulting in an overestimation of the surface temperature and heat transfer for such systems. Here we aim to obtain more quantitative insight into how surface cooling affects the Leidenfrost effect. We develop a technique based on Mach–Zehnder interferometry to investigate the surface cooling of a quartz plate by a Leidenfrost drop. The three-dimensional plate temperature field is reconstructed from interferometric data by an Abel inversion method using a basis function expansion of the underlying temperature field. By this method we are able to quantitatively measure the local cooling inside the plate, which can be as strong as 80 K. We develop a numerical model which shows good agreement with experiments and enables extending the analysis beyond the experimental parameter space. Based on the numerical and experimental results we quantify the effect of surface cooling on the Leidenfrost phenomenon. By focusing on the role of the solid surface we provide new insights into the Leidenfrost effect and demonstrate how to adjust current models to account for non-isothermal solids and use previously obtained isothermal scaling laws for the neck thickness and evaporation rate.

Recent evidence suggests that vitamin D deficiency is associated with CVD, impaired kidney function and proteinuria. To date, no study has evaluated these associations in renal transplant recipients (RTR) adjusting for body adiposity assessed by a ‘gold standard’ method. This study aimed to evaluate the vitamin D status and its association with body adiposity, CVD risk factors, estimated glomerular filtration rate (eGFR) and proteinuria in RTR, living in Rio de Janeiro, Brazil (a low-latitude city (22°54'10"S)), taking into account body adiposity evaluated by dual-energy X-ray absorptiometry (DXA). This cross-sectional study included 195 RTR (114 men) aged 47·6 (sd 11·2) years. Nutritional evaluation included anthropometry and DXA. Risk factors for CVD were hypertension, diabetes mellitus, dyslipidaemia and the metabolic syndrome. eGFR was evaluated using the Chronic Kidney Disease Epidemiology Collaboration equation. Serum 25-hydroxyvitamin D (25(OH)D) concentration was used to define vitamin D status as follows: 10 % (n 19) had vitamin D deficiency (<16 ng/ml), 43 % (n 85) had insufficiency (16–30 ng/ml) and 47 % (n 91) had sufficiency (>30 ng/ml). Percentage of body fat (DXA) was significantly associated with vitamin D deficiency independently of age, sex and eGFR. Lower 25(OH)D was associated with higher odds of the metabolic syndrome and dyslipidaemia after adjustment for age, sex and eGFR, but not after additional adjustment for body fat. Hypertension and diabetes were not related to 25(OH)D. Lower serum 25(OH)D was associated with increasing proteinuria and decreasing eGFR even after adjustments for age, sex and percentage of body fat. This study suggests that in RTR of a low-latitude city hypovitaminosis D is common, and is associated with excessive body fat, decreased eGFR and increased proteinuria.

We present the first submillimetric line survey of extragalactic sources carried out by APEX. The surveys cover the 0.8 mm atmospheric window from 270 to 370GHz toward NGC253, NGC4945 and Arp220. We found in NGC 253, 150 transitions of 26 molecules. For NGC 4945, 136 transitions of 24 molecules, and 64 transitions of 17 molecules for Arp 220. Column densities and rotation temperatures have been determinate using the Local Thermodinamical Equilibrium(LTE) line profile simulation and fitting in the MADCUBA IJ software. The differences found in ratios between the Galactic Center and the starburst galaxies NGC 4945 and NGC 253 suggest that the gas is less processed in the latter than in the Galactic Center. The high 18O/17O ratios in the galaxies NGC 4945 and NGC 253 suggest also material less processed in the nuclei of these galaxies than in the Galactic Center. This is consistent with the claim that 17O is a more representative primary product than 18O in stellar nucleosynthesis (Wilson and Rood 1994); Also, we did a Multitransitions study of H3O+ at 307GHz, 364GHz, 388GHz and 396GHz. From our non-LTE analysis of H3O+ in NGC253 with RADEX we found that the collisional excitation can not explain the observed intensity of the ortho 396 GHz line. Excitation by radiation from the dust in the Far-IR can roughly explain the observations if the H2 densities are relatively low. From the derived H3O+ column densities we conclude that the chemistry of this molecule is dominated by ionization produce by the starburst in NGC253 (UV radiation from the O stars) and Arp 220 (cosmic rays from the supernovae) and likely from the AGN in NGC4549 (X-rays ); Finally, we report, for the first time, the tentative detection of the molecular ion HCNH+ (precursor of HCN and HNC) toward a galaxy, NGC4945, abundance explain the claimed enhancement of HCN abundance in the AGN, due to the enhancement of the ionization rate by X-rays. The abundance is much larger than the Galactic center of the Milky Way.

Ca plays an essential role in bone development; however, little is known about its effect on intestinal gene expression in juvenile animals. In the present study, thirty-two weaned pigs (9·5 (sem 0·11) kg) were assigned to four diets that differed in Ca concentration (adequate v. high) and cereal composition (wheat–barley v. maize) to assess the jejunal and colonic gene expression of nutrient transporters, tight junction proteins, cytokines and pathogen-associated molecular patterns, nutrient digestibility, Ca balance and serum acute-phase response. To estimate the impact of mucosal bacteria on colonic gene expression, Spearman's correlations between colonic gene expression and bacterial abundance were computed. Faecal Ca excretion indicated that more Ca was available along the intestinal tract of the pigs fed high Ca diets as compared to the pigs fed adequate Ca diets (P< 0·05). High Ca diets decreased jejunal zonula occludens 1 (ZO1) and occludin (OCLN) expression, up-regulated jejunal expression of toll-like receptor 2 (TLR2) and down-regulated colonic GLUT2 expression as compared to the adequate Ca diets (P< 0·05). Dietary cereal composition up-regulated jejunal TLR2 expression and interacted (P= 0·021) with dietary Ca on colonic IL1B expression; high Ca concentration up-regulated IL1B expression with wheat–barley diets and down-regulated it with maize diets. Spearman's correlations (r>0·35; P< 0·05) indicated an association between operational taxonomic units assigned to the phyla Bacteroidetes, Firmicutes and Proteobacteria and bacterial metabolites and mucosal gene expression in the colon. The present results indicate that high Ca diets have the potential to modify the jejunal and colonic mucosal gene expression response which, in turn, interacts with the composition of the basal diet and mucosa-associated bacteria in weaned pigs.

Certain trypanosomatids co-evolve with an endosymbiotic bacterium in a mutualistic relationship that is characterized by intense metabolic exchanges. Symbionts were able to respire for up to 4 h after isolation from Angomonas deanei. FCCP (carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone) similarly increased respiration in wild-type and aposymbiotic protozoa, though a higher maximal O2 consumption capacity was observed in the symbiont-containing cells. Rotenone, a complex I inhibitor, did not affect A. deanei respiration, whereas TTFA (thenoyltrifluoroacetone), a complex II activity inhibitor, completely blocked respiration in both strains. Antimycin A and cyanide, inhibitors of complexes III and IV, respectively, abolished O2 consumption, but the aposymbiotic protozoa were more sensitive to both compounds. Oligomycin did not affect cell respiration, whereas carboxyatractyloside (CAT), an inhibitor of the ADP-ATP translocator, slightly reduced O2 consumption. In the A. deanei genome, sequences encoding most proteins of the respiratory chain are present. The symbiont genome lost part of the electron transport system (ETS), but complex I, a cytochrome d oxidase, and FoF1-ATP synthase remain. In conclusion, this work suggests that the symbiont influences the mitochondrial respiration of the host protozoan.