Pneumatic muscle actuator (PMA) similar to biological muscle is a new type of pneumatic actuator. The flexible manipulator based on PMAs was constructed to simulate the actual movement of the human upper arm. Considering the model errors and external disturbances, the fuzzing sliding mode control based on the saturation function was proposed. Compared with other fuzzy control methods, fuzzy control and saturation function are used to adjust the robust terms to improve the tracking accuracy and reduce the high-frequency chattering.

We numerically investigate turbulent Rayleigh–Bénard convection through two immiscible fluid layers, aiming to understand how the layer thickness and fluid properties affect the heat transfer (characterized by the Nusselt number $\mbox {Nu}$) in two-layer systems. Both two- and three-dimensional simulations are performed at fixed global Rayleigh number $\mbox {Ra}=10^8$, Prandtl number $\mbox {Pr}=4.38$ and Weber number $\mbox {We}=5$. We vary the relative thickness of the upper layer between $0.01 \le \alpha \le 0.99$ and the thermal conductivity coefficient ratio of the two liquids between $0.1 \le \lambda _k \le 10$. Two flow regimes are observed. In the first regime at $0.04\le \alpha \le 0.96$, convective flows appear in both layers and $\mbox {Nu}$ is not sensitive to $\alpha$. In the second regime at $\alpha \le 0.02$ or $\alpha \ge 0.98$, convective flow only exists in the thicker layer, while the thinner one is dominated by pure conduction. In this regime, $\mbox {Nu}$ is sensitive to $\alpha$. To predict $\mbox {Nu}$ in the system in which the two layers are separated by a unique interface, we apply the Grossmann–Lohse theory for both individual layers and impose heat flux conservation at the interface. Without introducing any free parameter, the predictions for $\mbox {Nu}$ and for the temperature at the interface agree well with our numerical results and previous experimental data.

This research communication aims to characterize the prevalence, molecular characterization and antimicrobial resistance profiling of Streptococcus agalactiae isolated from clinical mastitis in China. A total of 140 Strep. agalactiae isolates were identified from 12 out of 201 farms in 6 provinces, overall herd prevalence was 18.6% and the MLST analysis showed clonal complexes (CC) 103 and CC 67 were present in these herds with CC 103 predominant, accounting for 97.9%. Isolates were mostly sensitive to the tested antimicrobials: penicillin, ceftiofur, amoxi/clav, cefquinome, and vancomycin (100%), followed by cefalexin (97.9%), oxacillin (96.4%), enrofloxacin (95.7%), erythromycin (89.3%), and clindamycin (88.6%). Only 19.3 and 0.7% of isolates were sensitive to tetracycline and daptomycin, respectively, and sequence type (ST) 103 was most resistant to antimicrobials. In conclusion, CC 103 was the predominant subgroup of bovine mastitis Strep. agalactiae in China, and most antimicrobials apart from tetracycline and daptomycin were effective.

From geotechnical applications to space exploration, auger drilling is often used as a standard tool for soil sample collection, instrument installation and others. Focusing on granular flow associated with the rotary drilling process, we investigate the performance of auger drilling in terms of sampling efficiency, defined as the mass ratio of the soil sample collected in the coring tube to its total volume at a given penetration depth, by means of experiments, numerical simulations as well as theoretical analysis. The ratio of rotation to penetration speed is found to play a crucial role in the sampling process. A continuum model for the coupled granular flow in both coring and discharging channels is proposed to elucidate the physical mechanism behind the sampling process. Supported by a comparison with experimental results, the continuum model provides a practical way to predict the performance of auger drilling. Further analysis reveals that the drilling process approaches a steady state with constant granular flow speeds in both channels. In the steady state, sampling efficiency decreases linearly with the growth of the rotation to penetration speed ratio, which can be well captured by the analytical solution of the model. The analytical solution also suggests that the sampling efficiency is independent of gravity in the steady state, which has profound implications for extraterrestrial sample collection in future space missions.

This numerical study presents a simple but extremely effective way to considerably enhance heat transport in turbulent wall-bounded multiphase flows, namely by using oleophilic walls. As a model system, we pick the Rayleigh–Bénard set-up, filled with an oil–water mixture. For oleophilic walls, using only $10\,\%$ volume fraction of oil in water, we observe a remarkable heat transport enhancement of more than $100\,\%$ as compared to the pure water case. In contrast, for oleophobic walls, the enhancement is only of about $20\,\%$ as compared to pure water. The physical explanation of the heat transport increment for oleophilic walls is that thermal plumes detach from the oil-rich boundary layer and carry the heat with them. In the bulk, the oil–water interface prevents the plumes from mixing with the turbulent water bulk and to diffuse their heat. To confirm this physical picture, we show that the minimum amount of oil necessary to achieve the maximum heat transport is set by the volume fraction of the thermal plumes. Our findings provide guidelines of how to optimize heat transport in wall-bounded thermal turbulence. Moreover, the physical insight of how coherent structures are coupled with one of the phases of a two-phase system has very general applicability for controlling transport properties in other turbulent wall-bounded multiphase flows.

Eating architecture is a term that describes meal frequency, meal timing and meal size and the daily variation in each of these. The aim of this study was to determine the relationship between components of eating architecture on body fat and markers of glycaemic control in healthy adults at increased risk of type 2 diabetes (T2DM). Participants (n 73, 39 males, age 58·8 (8·1) years, BMI 33·4 (4·4) kg/m2) recorded food intake and wore accelerometers and continuous glucose monitors (CGM) for 7–14 d under free-living conditions. Body fat and glycated Hb (HbA1c) were also measured. The mean and day-to-day variation (calculated as the standard deviation during the monitoring period) of each component of eating architecture were calculated. Multivariable linear regression models were constructed for three separate outcome variables (body fat mass, mean CGM glucose and HbA1c) for each component of eating architecture before and after adjustment for confounders. Higher variability in the time of first meal consumption was associated with increased body fat mass after adjusting for confounders (β = 0·227, 95 % CI: 0·019, 0·434, P = 0·033). Increased variability in the time lag from waking to first meal consumption was also positively associated with increased HbA1c after adjustment (β = 0·285, 95 % CI: 0·040, 0·530, P = 0·023). Low day-to-day variability in first meal consumption was associated with lower body fat and improved glucose control in adults at increased risk of T2DM. Routine consumption of meals may optimise temporal regulation to anticipate and respond appropriately to a glucose challenge.

After fitting a topic model to 40,927 COVID-19–related paragraphs in 3,581 earnings calls over the period Jan. 22–Apr. 30, 2020, we obtain firm-level measures of exposure and response related to COVID-19 for 2,894 U.S. firms. We show that despite the large negative impact of COVID-19 on their operations, firms with a strong corporate culture outperform their peers without a strong culture. Moreover, these firms are more likely to support their community, embrace digital transformation, and develop new products than those peers. We conclude that corporate culture is an intangible asset designed to meet unforeseen contingencies as they arise.

We theoretically and numerically investigate the instabilities driven by diffusiophoretic flow, caused by a solutal concentration gradient along a reacting surface. The important control parameters are the Péclet number $Pe$, which quantifies the ratio of the solutal advection rate to the diffusion rate, and the Schmidt number $Sc$, which is the ratio of viscosity and diffusivity. First, we study the diffusiophoretic flow on a catalytic plane in two dimensions. From a linear stability analysis, we obtain that for $Pe$ larger than $8{\rm \pi}$ mass transport by convection overtakes that by diffusion, and a symmetry-breaking mode arises, which is consistent with numerical results. For even larger $Pe$, nonlinear terms become important. For $Pe > 16{\rm \pi}$, multiple concentration plumes are emitted from the catalytic plane, which eventually merge into a single larger one. When $Pe$ is even larger ($Pe \gtrsim 603$ for Schmidt number $Sc=1$), there are continuous emissions and merging events of the concentration plumes. The newly found flow states have different flow structures for different $Sc$: for $Sc\geqslant 1$, we observe the chaotic emission of plumes, but the fluctuations of concentration are only present in the region near the catalytic plane. In contrast, for $Sc<1$, chaotic flow motion occurs also in the bulk. In the second part of the paper, we conduct three-dimensional simulations for spherical catalytic particles, and beyond a critical Péclet number again find continuous plume emission and plume merging, now leading to a chaotic motion of the phoretic particle. Our results thus help us to understand the experimentally observed chaotic motion of catalytic particles in the high $Pe$ regime.

It is commonly accepted that the breakup criteria of drops or bubbles in turbulence is governed by surface tension and inertia. However, also buoyancy can play an important role at breakup. In order to better understand this role, here we numerically study two-dimensional Rayleigh–Bénard convection for two immiscible fluid layers, in order to identify the effects of buoyancy on interface breakup. We explore the parameter space spanned by the Weber number $5\leqslant We \leqslant 5000$ (the ratio of inertia to surface tension) and the density ratio between the two fluids $0.001 \leqslant \varLambda \leqslant 1$, at fixed Rayleigh number $Ra=10^8$ and Prandtl number $Pr=1$. At low $We$, the interface undulates due to plumes. When $We$ is larger than a critical value, the interface eventually breaks up. Depending on $\varLambda$, two breakup types are observed. The first type occurs at small $\varLambda \ll 1$ (e.g. air–water systems) when local filament thicknesses exceed the Hinze length scale. The second, strikingly different, type occurs at large $\varLambda$ with roughly $0.5 < \varLambda \leqslant 1$ (e.g. oil–water systems): the layers undergo a periodic overturning caused by buoyancy overwhelming surface tension. For both types, the breakup criteria can be derived from force balance arguments and show good agreement with the numerical results.

Meiosis is a highly conserved process, and is responsible for the production of haploid gametes and generation of genetic diversity. We previously identified the transferrin receptor (TFRC) in the proteome profile of mice neonatal testes, indicating the involvement of the TFRC in meiosis. However, the exact molecular role of the TFRC in meiosis remains unclear. In this study, we aimed to determine the function of the TFRC in neonatal testicular development by TFRC knockdown using the testis culture platform. Our results showed high TFRC expression in 2-week testes, corresponding to the first meiotic division. Targeting TFRC using morpholino oligonucleotides resulted in clear spermatocyte apoptosis. In addition, we used the chromosomal spread technique to show that a deficiency of TFRC caused the accumulation of leptotene and zygotene spermatocytes, and a decrease of pachytene spermatocytes, indicating early meiotic arrest. Moreover, the chromosomes of TFRC-deficient pachytene spermatocytes displayed sustained γH2AX association, as well as SYCP1/SYCP3 dissociation beyond the sex body. Therefore, our results demonstrated that the TFRC is essential for the progression of spermatocyte meiosis, particularly for DNA double-stranded break repair and chromosomal synapsis.

The upsurge in the number of people affected by the COVID-19 is likely to lead to increased rates of emotional trauma and mental illnesses. This article systematically reviewed the available data on the benefits of interventions to reduce adverse mental health sequelae of infectious disease outbreaks, and to offer guidance for mental health service responses to infectious disease pandemic. PubMed, Web of Science, Embase, PsycINFO, WHO Global Research Database on infectious disease, and the preprint server medRxiv were searched. Of 4278 reports identified, 32 were included in this review. Most articles of psychological interventions were implemented to address the impact of COVID-19 pandemic, followed by Ebola, SARS, and MERS for multiple vulnerable populations. Increasing mental health literacy of the public is vital to prevent the mental health crisis under the COVID-19 pandemic. Group-based cognitive behavioral therapy, psychological first aid, community-based psychosocial arts program, and other culturally adapted interventions were reported as being effective against the mental health impacts of COVID-19, Ebola, and SARS. Culturally-adapted, cost-effective, and accessible strategies integrated into the public health emergency response and established medical systems at the local and national levels are likely to be an effective option to enhance mental health response capacity for the current and for future infectious disease outbreaks. Tele-mental healthcare services were key central components of stepped care for both infectious disease outbreak management and routine support; however, the usefulness and limitations of remote health delivery should also be recognized.

The construction of halloysite spherical capsules (halloysite aerogels) was reported for the first time in our previous work. The excellent performance of the microcapsule in functional carrying was also found in our further research. In this work, the anti-icing surface was fabricated by using halloysite nanotubes and halloysite spherical microcapsules. The fabrication of the anti-icing coating was investigated, and the ice nucleation behavior of droplet on the coating surface was studied. The modified halloysite nanotubes (F-HNTs) and the modified halloysite microcapsules (F-HAs) were characterized by Fourier-transform infrared spectroscopy, thermal gravimetric, and pore size distribution. The results show that the introduction of F-HNTs and F-HAs have successfully formed a micro-nano structure on the coating surface with superhydrophobicity performance. The icing temperature of the coating has decreased 2.3 °C compared with bare glass, and the ice adhesion strength has decreased 82%. According to the ice dynamic mechanics, the ice nucleation rate on the coating is significantly reduced, thus the halloysite microcapsule coating has good icephobic performance.

There is growing interest globally in using real-world data (RWD) and real-world evidence (RWE) for health technology assessment (HTA). Optimal collection, analysis, and use of RWD/RWE to inform HTA requires a conceptual framework to standardize processes and ensure consistency. However, such framework is currently lacking in Asia, a region that is likely to benefit from RWD/RWE for at least two reasons. First, there is often limited Asian representation in clinical trials unless specifically conducted in Asian populations, and RWD may help to fill the evidence gap. Second, in a few Asian health systems, reimbursement decisions are not made at market entry; thus, allowing RWD/RWE to be collected to give more certainty about the effectiveness of technologies in the local setting and inform their appropriate use. Furthermore, an alignment of RWD/RWE policies across Asia would equip decision makers with context-relevant evidence, and improve timely patient access to new technologies. Using data collected from eleven health systems in Asia, this paper provides a review of the current landscape of RWD/RWE in Asia to inform HTA and explores a way forward to align policies within the region. This paper concludes with a proposal to establish an international collaboration among academics and HTA agencies in the region: the REAL World Data In ASia for HEalth Technology Assessment in Reimbursement (REALISE) working group, which seeks to develop a non-binding guidance document on the use of RWD/RWE to inform HTA for decision making in Asia.

Impact of microspheres on liquid surfaces is a universal phenomenon in nature and in industrial processes. However, most relevant studies have mainly focused on the sphere's vertical impact. Herein, we present the first observation on the oblique impact of microspheres on the surface of quiescent liquid using high-speed microphotography. The sphere motion and liquid surface distortion after the oblique impact are basically different from those after a vertical impact. The sphere rotates and its trajectory deviates from the impact direction during the oblique impact process, while the non-axisymmetric liquid surface distortion experiences an evolution from half-cavity to full-cavity patterns. The dependence of motions of the sphere and the three-phase contact line on the impact angle $\alpha$ and Weber number are investigated, and the scaling laws for the sphere's penetration time and penetration depth are given. We provide a phase diagram with respect to the Weber number and impact angle that describes the observed impact modes of submergence and oscillation, which shows that the critical Weber number between two impact modes increases when the impact angle decreases. Additionally, a scaling model is established based on energy balance to distinguish different impact modes. The model indicates that the critical Weber number for the microsphere's oblique impact is equal to $1/{\rm sin}\,\alpha$ times that for vertical impact, agreeing well with the experimental results.