To send content items to your account,
please confirm that you agree to abide by our usage policies.
If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account.
Find out more about sending content to .
To send content items to your Kindle, first ensure email@example.com
is added to your Approved Personal Document E-mail List under your Personal Document Settings
on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part
of your Kindle email address below.
Find out more about sending to your Kindle.
Note you can select to send to either the @free.kindle.com or @kindle.com variations.
‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi.
‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.
A small eddy viscosity or mass diffusivity that varies with height has been found to have unexpected effects on the Kelvin–Helmholtz (KH) instability of a stably stratified shear layer near the neutral stability boundary. In particular, varying viscosity can increase the growth rate of the instability in contrast to the effect of uniform viscosity. Here, these results are extended to parameter ranges relevant in many geophysical and engineering contexts. We find that linearization of the viscous terms based on the assumption of weak viscosity/diffusivity is valid for non-dimensional values (inverse Reynolds number) up to
. Decreasing the Richardson number far below its critical value
can change, or even reverse, the effects of eddy viscosity and diffusivity. A primary goal is to explain the unexpected destabilization by viscosity. Varying viscosity affects vorticity (and other fluid properties) in a manner identical to advection with an advecting velocity equal to minus the gradient of viscosity. Destabilization occurs when this viscous ‘advection’ reinforces the vorticity distribution of a growing mode.
In contrast to non-vegetarians, vegetarians consume more legumes and meat analogues as sources of protein to substitute for meat intake. The present study aimed to assess the association between foods with high protein content (legumes, meat, meat analogues) by dietary pattern (vegetarians, non-vegetarians) and hip fracture incidence, adjusted for selected lifestyle factors.
A prospective cohort of Adventist Health Study-2 (AHS-2) enrollees who completed a comprehensive lifestyle and dietary questionnaire between 2002 and 2007.
Every two years after enrolment, a short questionnaire on hospitalizations and selected disease outcomes including hip fractures was sent to these members.
Respondents (n 33 208) to a baseline and a follow-up questionnaire.
In a multivariable model, legumes intake of once daily or more reduced the risk of hip fracture by 64 % (hazard ratio = 0·36, 95 % CI 0·21, 0·61) compared with those with legumes intake of less than once weekly. Similarly, meat intake of four or more times weekly was associated with a 40 % reduced risk of hip fracture (hazard ratio = 0·60, 95 % CI 0·41, 0·87) compared with those whose meat intake was less than once weekly. Furthermore, consumption of meat analogues once daily or more was associated with a 49 % reduced risk of hip fracture (hazard ratio = 0·51, 95 % CI 0·27, 0·98) compared with an intake of less than once weekly.
Hip fracture incidence was inversely associated with legumes intake and, to a lesser extent, meat intake, after accounting for other food groups and important covariates. Similarly, a high intake of meat analogues was associated with a significantly reduced risk of hip fracture.
The effect of non-zero, but small, viscosity and diffusivity on the marginal stability of a stably stratified shear flow is examined by making perturbations around the neutral solution for an inviscid and non-diffusive flow. The results apply to turbulent flows in which horizontal and vertical turbulent transports of momentum and buoyancy are represented by eddy coefficients of viscosity and diffusivity that vary in the vertical (
) direction. General expressions are derived for the modified phase speed and the growth rate of small disturbances as a function of wavenumber. To first order in their coefficients, the effect on the phase speed of adding viscosity and diffusivity is zero. Growth rates are found for two mean flows when the horizontal or vertical coefficients of viscosity and diffusivity vary in
in such a way that the rates can be found analytically. The first flow, denoted as a ‘Holmboe flow’, has a velocity and density interface: the mean horizontal velocity and the density are both proportional to
is proportional to the inverse of the interface thickness. The second, ‘Drazin flow’, has a similar velocity variation in
but uniform density gradient. The analytical results compare favourably with numerical calculations. Small horizontal coefficients of viscosity and diffusivity may affect disturbances to the flow in opposite ways. Although the effect of uniform vertical coefficients of viscosity is to decrease the growth rates, and uniform vertical coefficients of diffusivity increase them, cases are found in which, with suitably chosen
dependence, vertical coefficients of viscosity (or diffusivity) may cause a previously neutral disturbance to grow (or to diminish); viscosity may destabilize a stably stratified shear flow. The introduction of viscosity and diffusivity may consequently increase the critical Richardson number to a value exceeding
. While some patterns of behaviour are apparent, no simple rule appears to hold about whether flows that are neutral in the absence of these effects (viscosity or diffusivity) will be stabilized or destabilized when they are added. One such rule, namely our conjecture that viscosity is always stabilizing and that diffusivity is destabilizing, is explicitly refuted.
The X-linked telomeric P elements (TPs) TP5 and TP6 regulate the activity of the entire P element family because they are inserted in a major locus for the production of Piwi-interacting RNAs (piRNAs). The potential for this cytotype regulation is significantly strengthened when either TP5 or TP6 is combined with a non-telomeric X-linked or autosomal transgene that contains a P element. By themselves, none of the transgenic P elements have any regulatory ability. Synergism between the telomeric and transgenic P elements is much greater when the TP is derived from a female. Once an enhanced regulatory state is established in a female, it is transmitted to her offspring independently of either the telomeric or transgenic P elements – that is, it works through a strictly maternal effect. Synergistic regulation collapses when either the telomeric or the transgenic P element is removed from the maternal genotype, and it is significantly impaired when the TPs come from stocks heterozygous for mutations in the genes aubergine, piwi or Su(var)205. The synergism between telomeric and transgenic P elements is consistent with a model in which P piRNAs are amplified by alternating, or ping-pong, targeting of primary piRNAs to sense and antisense P transcripts, with the sense transcripts being derived from the transgenic P element and the antisense transcripts being derived from the TP.
The often-cited estimate that about 30% of our diet depends directly or indirectly on crops pollinated by animals (McGregor 1976) may not hold with more recent and future analyses (Klein et al. 2007). But much of the variety in our diet beyond staples such as wind-pollinated grains depends on animals, especially bees, for pollination. Such pollinator-dependent crops include many fruits (pome, stone), berries (cane, bramble), cucurbits (melons, squashes), nuts (almond, macadamia) and vegetable, oil, and forage crop seeds.
Analyses of primary data from 200 countries found that over 75% of 87 globally important food crops (fruits, vegetables, and seeds) are dependent on animal pollination (Klein et al. 2007). These authors state that this represents about 35% of human food production. They also evaluated habitat management for pollination for nine crops and found that intensification of agriculture put wild bees and pollination services at risk. However, analyses of FAO data by Aizen et al. (2008) show similar trends of increased yields in both pollinator-dependent and nondependent crops. They conclude that this does not support the contention that animal-pollinated crops are currently being affected by pollinator shortages at a global scale. However, they warn that if the disproportionate increase in area cultivated with pollinator-dependent crops continues, dependency on services from dwindling pollinator populations will increase in future.
The Taylor–Goldstein (T–G) equation is extended to include the effects of small-scale turbulence represented by non-uniform vertical and horizontal eddy viscosity and diffusion coefficients. The vertical coefficients of viscosity and diffusion, and , respectively, are assumed to be equal and are expressed in terms of the buoyancy frequency of the flow, , and the dissipation rate of turbulent kinetic energy per unit mass, , quantities that can be measured in the sea. The horizontal eddy coefficients, and , are taken to be proportional to the dimensionally correct form, , found appropriate in the description of horizontal dispersion of a field of passive markers of scale . The extended T–G equation is applied to examine the stability and greatest growth rates in a turbulent shear flow in stratified waters near a sill, that at the entrance to the Clyde Sea in the west of Scotland. Here the main effect of turbulence is a tendency towards stabilizing the flow; the greatest growth rates of small unstable disturbances decrease, and in some cases flows that are unstable in the absence of turbulence are stabilized when its effects are included. It is conjectured that stabilization of a flow by turbulence may lead to a repeating cycle in which a flow with low levels of turbulence becomes unstable, increasing the turbulent dissipation rate and so stabilizing the flow. The collapse of turbulence then leads to a condition in which the flow may again become unstable, the cycle repeating. Two parameters are used to describe the ‘marginality’ of the observed flows. One is based on the proximity of the minimum flow Richardson number to the critical Richardson number, the other on the change in dissipation rate required to stabilize or destabilize an observed flow. The latter is related to the change needed in the flow Reynolds number to achieve zero growth rate. The unstable flows, typical of the Clyde Sea site, are relatively further from neutral stability in Reynolds number than in Richardson number. The effects of turbulence on the hydraulic state of the flow are assessed by examining the speed and propagation direction of long waves in the Clyde Sea. Results are compared to those obtained using the T–G equation without turbulent viscosity or diffusivity. Turbulence may change the state of a flow from subcritical to supercritical.
TP5, a P element inserted in the telomere-associated sequences of the X chromosome, represses the excision of other P elements in the germ line through a combination of maternal and zygotic effects. The maternal component of this repression is impaired by heterozygous mutations in the aubergine and Suppressor of variegation 205 genes; one mutation in the piwi gene also appears to impair repression. In the female germ line, the level of TP5 mRNA is increased by these impairing mutations. The impairing aubergine and piwi mutations also increase the level of germ-line mRNA from CP, a transgene that encodes the P-element transposase; however, the Suppressor of variegation 205 mutation does not. These findings are discussed in terms of a model of P-element regulation that involves post-transcriptional and chromatin re-organizing events mediated by maternally transmitted small RNAs derived from the telomeric P element.
The X-linked telomeric P elements TP5 and TP6 interact synergistically with non-telomeric P elements to repress hybrid dysgenesis. In this repression, the telomeric P elements exert maternal effects, which, however, are not sufficient to establish synergism with the non-telomeric P elements. Once synergism is established, the capacity to repress dysgenesis in the offspring of a cross persists for at least two generations after removing the telomeric P element from the genotype. At the molecular level, synergism between telomeric and non-telomeric P elements is correlated with effective elimination of P-element mRNA in the germ line. Maternally transmitted mutations in the genes aubergine, piwi and Suppressor of variegation 205 [Su(var)205] block the establishment of synergism between telomeric and non-telomeric P elements, and paternally transmitted mutations in piwi and Su(var)205 disrupt synergism that has already been established. These findings are discussed in terms of a model of cytotype regulation of P elements based on Piwi-interacting RNAs (piRNAs) that are amplified by cycling between sense and antisense species.
Strains carrying the X-linked telomeric P elements TP5 or TP6 varied in their ability to repress hybrid dysgenesis. The rank ordering of these strains was consistent across different genetic assays and was not related to the type of telomeric P element (TP5 or TP6) present. Strong repression of dysgenesis was associated with weak expression of mRNA from the telomeric P element and also with a reduced amount of mRNA from a transposase-producing P element contained within a transgene inserted on an autosome. A strictly maternal component of repression, transmitted independently of the telomeric P element, was detected in the daughters but not the sons of females from the strongest repressing strains. However, this effect was seen only when dysgenesis was induced by crossing these females to males from a P strain, not when it was induced by crossing them to males homozygous for a single transposase-producing P element contained within a transgene. These findings are consistent with the hypothesis that the P cytotype, the condition that regulates P elements, involves an RNA interference mechanism mediated by piRNAs produced by telomeric P elements such as TP5 and TP6 and amplified by RNAs produced by other P elements.
Posttraumatic stress disorder (PTSD) has been associated with neuropsychological impairments across multiple domains, but consensus regarding the cognitive profile of PTSD has not been reached. In this study of women with PTSD related to intimate partner violence (n = 55) and healthy, demographically similar comparison participants (NCs; n = 20), we attempted to control for many potential confounds in PTSD samples. All participants were assessed with a comprehensive neuropsychological battery emphasizing executive functioning, including inhibition, switching, and abstraction. NCs outperformed PTSD participants on most neuropsychological measures, but the differences were significant only on speeded tasks (with and without executive functioning components). The PTSD group’s mean performance was within the average range on all neuropsychological tests. Within the PTSD group, more severe PTSD symptoms were associated with slower processing speed, and more severe dissociative symptoms were associated with poorer reasoning performance. These results suggest that women with PTSD related to intimate partner violence demonstrate slower than normal processing speed, which is associated with the severity of psychiatric symptoms. We speculate that the cognitive slowing seen in PTSD may be attributable to reduced attention due to a need to allocate resources to cope with psychological distress or unpleasant internal experiences. (JINS, 2009, 15, 879–887.)
Maternal malaria and under-nutrition are established risk factors for small-for-gestational-age (SGA) births; however, whether malaria is associated with intrauterine growth restriction (IUGR) is unknown. We investigated IUGR risk among 177 HIV-negative pregnant women enrolled in a longitudinal ultrasound study conducted in Democratic Republic of Congo from May 2005 to May 2006. Malaria infection, maternal anthropometrics, and ultrasound estimated fetal weight were measured monthly. All positive malaria cases were treated and intermittent presumptive therapy (IPTp) provided. Log-binomial regression models for IUGR were fitted using generalized estimating equations to account for statistical clustering of repeat IUGR measurements. Twenty-nine percent of fetuses experienced an episode of IUGR with the majority occurring in the third trimester. The risk of IUGR associated with malaria was greatest after three or more cumulative infections (RR 3·3, 95% CI 1·3–8·2) and was two- to eight-fold higher among women with evidence of under-nutrition. Receiving antimalarial treatment in the previous month (for IPTp or treatment) was significantly protective against IUGR (RR 0·5, 95% CI 0·3–0·7). The interaction observed between malaria and under-nutrition suggests that antenatal programmes in malaria endemic areas should incorporate nutritional screening and supplementation in addition to IPTp.
Evidence suggesting that a diet high in fruits and vegetables may be beneficial to bone health has sparked interest in the potential benefit of a vegetarian diet. However, other studies have raised a question regarding the adequacy of protein in such a diet.
The aim of the present study was to take a whole foods approach in examining the effects of foods high in protein on the risk of wrist fracture (WF) in a cohort with a significant proportion consuming a meat-free diet.
A cohort study of women who completed two lifestyle surveys 25 years apart.
One thousand eight hundred and sixty-five peri- and postmenopausal women at the time of the first survey.
There was a significant interaction between meat consumption and foods high in vegetable protein. Among vegetarians, those who consumed the least vegetable protein intake were at highest risk for fracture. However, increasing levels of plant-based high-protein foods decreased WF risk, with a 68% reduction in risk (hazard ratio (HR) = 0.32, 95% confidence interval (CI) 0.13–0.79) in the highest intake group. Among those with lowest vegetable protein consumption, increasing meat intake decreased the risk of WF, with the highest consumption decreasing risk by 80% (HR = 0.20, 95% CI 0.06–0.66).
The finding that higher consumption frequencies of foods rich in protein were associated with reduced WF supports the importance of adequate protein for bone health. The similarity in risk reduction by vegetable protein foods compared with meat intake suggests that adequate protein intake is attainable in a vegetarian diet.
Red Maasai and Dorper sheep were evaluated for their resistance to gastro-intestinal (GI) nematode parasites (predominantly Haemonchus contortus), productivity and productive efficiency (assessed on a metabolizable energy basis) in experiments undertaken at the Kenyan coast (sub-humid environment) and the Kenya highlands (semi-arid environment). In both ewes and lambs there were few significant genotype by environment (G X E) interactions for either resistance (assessed by faecal egg counts-FEC) or resilience (assessed by blood packed red cell volume-PCV) to GI nematodes. Red Maasai sheep were consistently more resistant (low FEC) and more resilient (high PCV) than Dorper sheep. However, there were significant G X £ interactions for ewe reproductive performance and for ewe and lamb mortality rates and live weights. These interactions were due to very poor performance of the Dorper compared to the Red Maasai in the sub-humid coastal environment and to the much improved performance of the Dorper in the semi-arid environment. When these component traits were combined into estimates of flock productivity and productive efficiency there were highly significant GXE interactions with the Red Maasai sheep being considerably more efficient than Dorper sheep in the sub-humid environment, while in the semi-arid environment there was a negligible breed difference in productive efficiency. The results are discussed in terms of breeding strategies for smallholder farmers and pastoralists managing sheep in low-input systems in sub-humid and semi-arid environments.
Resistance and resilience to naturally acquired gastro-intestinal (GI) nematode parasite infections (predominantly Haemonchus contortus) were studied in 1785 lambs born over six lambings (1991 to 1996) consisting of 212 Red Maasai, 311 Dorper and 1262 crossbred (Red Maasai-Dorper) lambs in the sub-humid coastal region of Kenya. These lambs were the progeny of 41 Dorper and 35 Red Maasai rams. Live weights (LWT), blood packed cell volume (PCV) and faecal egg counts (FEC) were recorded at 1- to 2-monthly intervals from birth until the lambs were about one year of age. Red Maasai were more resistant and resilient post weaning to infections with GI nematodes than Dorper lambs as shown by their significantly lower FEC and their significantly higher PCV, respectively. An increasing proportion of Red Maasai genes in the crossbred lambs was associated with decreased FEC and higher PCV, but there was no heterosis for logarithm-transformed FEC (LFEC) or PCV. From one month of age Red Maasai lambs were significantly lighter than Dorper lambs by about 1 kg, but Red Maasai lambs had significantly lower lamb mortality rate from birth to 12 months of age (proportionately 0·30 and 0·66, respectively). Heritability estimates from a repeated measures analysis for records taken at 6 and 8 months of age were 0·14 (s.e. 0·05) for PCV from an animal model and 0·12 (s.e. 0·05) for LFEC from a sire model. The heritability estimate for LFEC from a repeated measures analysis including the four measurements recorded between 6 and 12 months of age was significantly higher (P < 0·05) for Dorper-sired lambs (0·15, s.e. 0·05 for an animal model and 0·19, s.e. 0·07 for a sire model) than for Red Maasai-sired lambs (0·00 and 0·01, s.e. 0·02). The phenotypic and genetic correlations between PCV and LFEC were moderately to highly negative and averaged –0·34 and –0·81, respectively. None of the genetic correlation estimates between LWT and PCV and LWT and LFEC for lambs post weaning were significantly different from zero. The heritability estimates for PCV and LFEC have important implications for within-breed genetic improvement programmes: for the Red Maasai, improvement should concentrate on resilience (e.g. selection for high PCV); for the Dorper, selection should be feasible for both improved resistance (low FEC) and resilience (high PCV).
We hypothesised that the observed acceleration in the kinetics of exercise on-transient oxygen uptake (V˙O2) of five older humans (77 ± 7 years (mean ± S.D.) following 9 weeks of single-leg endurance exercise training was due to adaptations at the level of the muscle cell. Prior to, and following training, subjects performed constant-load single-limb knee extension exercise. Following training V˙O2 kinetics (phase 2, τ) were accelerated in the trained leg (week 0, 92 ± 44 s; week 9, 48 ± 22 s) and unchanged in the untrained leg (week 0, 104 ± 43 s; week 9, 126 ± 35 s). The kinetics of mean blood velocity in the femoral artery were faster than the kinetics of V˙O2, but were unchanged in both the trained (week 0, 19 ± 10 s; week 9, 26 ± 11 s) and untrained leg (week 0, 20 ± 18 s; week 9, 18 ± 10 s). Maximal citrate synthase activity, measured from biopsies of the vastus lateralis muscle, increased (P < 0.05) in the trained leg (week 0, 6.7 ± 2.0 µmol (g wet wt)-1 min-1; week 9, 11.4 ± 3.6 µmol (g wet wt)-1 min-1) but was unchanged in the untrained leg (week 0, 5.9 ± 0.5 µmol (g wet wt)-1 min-1; week 9, 7.9 ± 1.9 µmol (g wet wt)-1 min-1). These data suggest that the acceleration of V˙O2 kinetics was due to an improved rate of O2 utilisation by the muscle, but was not a result of increased O2 delivery. Experimental Physiology (2001) 86.5, 659-665.
A study was carried out from 1992 to 1996 to compare the resistance to naturally acquired gastro-intestinal (GI) nematode parasite infections (predominantly Haemonchus contortus) of Galla and Small East African goats in the sub-humid coastal region of Kenya. A total of 204 Galla and 349 Small East African (SEA) kids were born from five kiddings. These were the progeny of 18 Galla and 17 SEA bucks. Live weights (LWT), blood packed-cell volume (PCV) and faecal egg count (FEC) were recorded at 1- to 2-month intervals from birth to about 14 months of age. The SEA kids were more resistant to GI nematode parasites than Galla kids as shown by their significantly lower FEC (P < 0·001) in the post-weaning period (8- to 14-month-old kids) and lower mortality from birth to 14 months of age (P < 0·05). There was no significant (P > 0·05) breed effect on PCV, but Galla kids were significantly heavier (P < 0·001) at all measurement times between birth and 14 months of age. Heritability estimates for LWT, PCV and FEC at the different sampling times were characterized by high standard errors. Heritability estimates for records taken at 4·5 and 8 months of age from a repeated measures analysis were 0·18 (s.e.0·08) for PCV and 0·13 (s.e.0·07) for logarithm-transformed FEC. The phenotypic and genetic correlation estimates between PCV and LFEC were moderately to highly negative and averaged –0·36 and –0·53, respectively. The results are discussed in relation to the limited evidence for resistance to GI nematode infections in goats and compared with the much stronger evidence for resistance in sheep.
Reliable radiocarbon dating depends upon well-defined samples. We have been investigating whether or not reliable 14C dates can be obtained directly from sub-fossil insect cuticle or biochemical fractions derived from it. Initial carbon and nitrogen stable isotope measurements on sub-fossil insect chitin from species with known feeding behaviors found within a single site (St Bees, Cumbria) clustered in a manner reminiscent of trophic level effects seen in terrestrial ecosystems. Although this finding implied some chemical stability, the measurement of CN ratios from the same samples indicated compositional variability. In addition, 14C dates obtained from these same samples were different from dates obtained from plant macrofossils found at the same depth. We have experimented with protocols designed to biochemically reduce chitin to its principle carbohydrate component glucosamine with the aim of using this compound to generate reliable 14C dates. Solvent extractions of sub-fossil chitin were carried out to remove both endogenous and exogenous lipid-soluble materials. Base hydrolysis reactions designed to extract polypeptides retained surprisingly high levels of contaminating amino acids. Proteinase K enzyme treatment had little affect on the level of amino acid contamination. Strong acid hydrolysis reactions designed to depolymerize chitin to glucosamine yielded only 5% glucosamine. Clearly alternative methods of chitin depolymerization must be identified before the purification and 14C dating of glucosamine from sub-fossil chitin becomes practical.
Magnetization measurements, transmission electron microscopy (TEM), and high-resolution micro-x-ray fluorescence (μ-XRF) using a synchrotron radiation source (Advanced Photon Source) were used to examine Fe3O4 particle agglomerates of nominally 10-nm particles at low concentrations (down to 0.03%) in thick epoxy resin samples. The magnetization measurements showed that at low concentrations (<0.5%) the magnetite particles, although closely packed in the agglomerates, did not interact magnetically. Predicated on a 2-μm sample step scan, the μ-XRF results were compatible with the presence of spherical agglomerates due to magnetostatic attraction, and these ranged in size from 100 to several thousand nanometers, as observed in TEM measurements. At smaller step scans the resolution could be significantly improved. Thus, the synchroton μ-XRF method was very useful in detecting very small concentrations of particles in thick samples and could probably be used to detect particles in amounts as low as 10−16 g.