Boar taint, an off-odor and an off-flavor in the meat from some uncastrated male pigs, is due to high levels of the testicular steroid hormone, androstenone, and the indole, skatole. Thus far, there are no known methods for controlling both androstenone and skatole through dietary means. We tested the adsorbent agents, cholestyramine (CH), activated carbon (AC), tween-60 (Tween), bentonite (BNT) and polyvinylpolypyrrolidone (PVPP) for binding androstenone, estrone (E1), estrone sulfate (E1S) and skatole from buffer solutions in an in vitro system. The goal was to determine the potential utility of these binding agents as feed additives to control boar taint. Michaelis–Menten analysis was utilized to determine the effectiveness of the adsorbents. At pH 7.4, E1S was bound to AC and CH with the highest Bmax (maximum binding), whereas Tween and AC had the greatest Bmax for E1. The Bmax for skatole at pH 7.4 was highest for AC, CH and PVPP. AC had a higher Bmax for androstenone than CH and Tween. The Bmax values at pH 3.0 with E1S for AC and CH were essentially 100%, whereas the binding of Tween to E1S at pH 3.0 decreased by 49.5% from binding at pH 7.4 (P < 0.05). The Adint values, which represent efficiency of binding, illustrated that AC bound E1, androstenone and skatole with greater efficiency than the other binding agents at pH 7.4, whereas AC bound E1S as efficiently as CH. We conclude that AC was the most effective adsorbent agent for binding E1, E1S, androstenone and skatole in vitro, followed by CH, Tween, PVPP and lastly BNT. These adsorbent agents may be useful for binding boar taint compounds in in vivo studies to decrease the risk of boar taint.