In this paper, the design, simulation, and fabrication of a novel printed rectangular slot antenna with a band-notched function suitable for 2.4 GHz wireless local area network (WLAN) and ultra-wideband (UWB) applications is presented and investigated. Two pairs of slits are introduced into the ground plane to realize band-notched function, by tuning the position, length, and width of which a suitable rejected frequency band can be obtained. To improve the impedance matching, a rectangular cut is also made in the ground plane so that the antenna can cover 2–12 GHz frequency range. According to the measured results, the proposed antenna has a large bandwidth totally satisfying the requirement of 2.4 GHz WLAN and UWB systems, while providing the required band-notch function from 5.1 to 5.9 GHz. The study of transfer function and time-domain characteristics also indicates the band-notched function of the antenna. The radiation patterns display nearly omni-directional performance and the antenna gain is stable except in the rejected frequency band (5.1–5.9 GHz). Moreover, group delays are within 1.5 ns except for the notch band. These features make it a promising candidate for UWB wireless applications. Details of this antenna are described, and the experimental results of the constructed prototype are given.