Two or more pesticides together may produce a growth response in plants that is not predictable by their individual or independent toxicities. This unpredicted (dependent) response results from an interaction, a concept that usually is not easily interpreted. Dependent responses are further complicated by the fact that they can be either synergistic or antagonistic. Several methods exist for identifying and measuring phytotoxic interactions. Nearly all methods have certain shortcomings, however. Additive and multiplicative models (mathematical expressions) are the two basic approaches to determining pesticide interactions. The two-parameter, isobole, and calculus methods axe additive; whereas, Colby and regression estimate are multiplicative models. Regression estimate analysis considers deviations due to experimental errors, and a statistical significance can be attached to the interaction magnitude, thereby overcoming the deficiencies of the Colby method, but both methods seem to be limited to response data in which the combined pesticide concentration is the sum of the individual pesticide concentrations. The two-parameter method seems to be limited to response data in which the combined concentration is equal to the individual pesticide concentration and to response data in which a pesticide concentration necessary to produce a 50% of control value is interpolated rather than extrapolated. The calculus method is a mathematical expression of the growth response, and interaction is measured by derivation of the equation obtained. The calculus method is difficult to interpret and has a major weakness because it depends upon the multiple regression equation of the observed data. The regression estimate method is recommended as a reasonable approach to interpretation of interaction type data, with a SAS language computer program available from the author.

A phytotoxin that inhibited seedling root growth was isolated and purified from rhizomes of quackgrass [Agropyron repens (L.) Beauv.]. Aqueous solutions of the phytotoxin (0.1%, w/v) significantly inhibited the seedling root growth of corn (Zea mays L. ‘New Jersey 8’), oat (Avena sativa L. ‘Clintland 60’), cucumber (Cucumis sativus L. ‘Straight Eight’), and alfalfa (Medicago sativa L. ‘Vernal’). Growth inhibition was not due to extremes of pH or osmotic potential of the inhibitor solutions. The phytotoxin was isolated and purified from a methanol:water extract of dried and ground rhizomes by adsorption and elution from charcoal. A golden-brown, crystalline substance was obtained that was soluble in water, partly soluble in semipolar solvents, and insoluble in nonpolar solvents. Thin layer chromatography (TLC) and high pressure liquid chromatography (HPLC) suggested that a single compound was present. Elemental analysis showed that it contained 42.17% carbon, 5.06% hydrogen, 38.28% oxygen, 4.02% nitrogen, 2.03% sulfur, 0.03% chlorine, and 0.03% phosphorus. The compound was tentatively identified as a glycoside with a molecular weight of 460. Its ultraviolet (UV) absorption spectrum showed an intense maximum at 307 nm.

Field and greenhouse experiments were established to determine the phytotoxicity of Canada thistle [Cirsium arvense (L.) Scop.] residue on crop growth. Field examination of infestations showed that as Canada thistle shoots increased in an area, the number of kochia [Kochia scoparia (L.) Schrad.], marshelder (Iva xanthifolia Nutt.) and foxtail barley (Hordeum jubatum L.) plants decreased. Conversely, as Canada thistle shoots decreased, the aforementioned annual and perennial plants increased. In greenhouse studies, roots and shoots of Canada thistle that were mixed with soil reduced the growth of sugarbeet (Beta vulgaris L. ‘Mono Hy D2’), wheat (Triticum aestivum L. ‘Centurk’), alfalfa (Medicago sativa L. ‘Dawson’), and Canada thistle seedlings. Corn (Zea mays L. ‘Jacques No. 1004’) and dry edible beans (Phaseolus vulgaris L. ‘Great Northern No. 59’) grown in soil treated with Canada thistle residue were affected to a lesser extent. When Canada thistle residue was mixed with soil, crop growth was inversely proportional to the amount of Canada thistle residue added to the soil. Both roots and shoots of Canada thistle were toxic to crops when mixed with the soil. The effects of Canada thistle residue on crop growth lasted for about 60 days. Neither autoclaving residue and soil nor fertilization of soil had any effect on residue toxicity. Canada thistle leaf leachate inhibited the growth of sugarbeets watered daily with the leachate.

Combined effects of herbicides, herbicide rates, and cultivation for weed control in soybeans [Glycine max (L.) Merr. ‘Williams’] were studied using full and one-half rates of either chloramben (3-amino-2,5-dichlorobenzoic acid) or alachlor [2-chloro-2′,6′-diethyl-N-(methoxymethyl)acetanilide] + linuron [3-(3,4-dichlorophenyl)-1-methoxy-1-methylurea] with no, one, and two cultivations. The full rate was 2.2 kg/ha for alachlor and chloramben and 0.70 kg/ha for linuron. Alachlor + linuron was better than chloramben, and the full rate was better than the one-half rate. One or two cultivations were better than no cultivation. Cultivations were more effective when used with alachlor + linuron than when used with chloramben or when used alone. Alachlor + linuron at the full rate with one or two cultivations produced the best soybean yields. A one-half rate of alachlor + linuron with one or two cultivations yielded the same as a full rate of alachlor + linuron alone or with one cultivation, chloramben at one-half rate with two cultivations, and chloramben at a full rate with two cultivations. Cultivations can be effective by increasing weed control and yields when herbicide rate or effectiveness has been reduced. Use of a cultivation in addition to the preemergence herbicides used in this study is necessary for improved weed control and yields.

The diphenyl ether, oxyfluorfen [2-chloro-1-(3-ethoxy-4-nitrophenoxy)-4-(trifluoromethyl)benzene], exerts a very strong and rapid bleaching effect upon intact microalgae such as Scenedesmus acutus. Carotenoids, and subsequently chlorophylls, are destroyed concurrently with ethane formation and inhibition of photosynthetic oxygen evolution. These herbicidal effects are not observed before an activation time of approximately 2 h, during which photosynthetic electron transport is necessary. Diuron [3-(3,4-dichlorophenyl)-1,1-dimethylurea] inhibits oxyfluorfen activity during this activation time, but not thereafter. Isolated spinach chloroplasts (Spinacia oleracea ‘Atlanta’) evolve ethane after a light-incubation phase in the presence of oxyfluorfen as well as paraquat (methylviologen, 1,1′-dimethyl-4,4′-bipyridinium ion). Depending on their chemical constitution, diphenyl ethers apparently act differently and multifunctionally. The effects described for oxyfluorfen are believed to represent the primary mode of action of bleaching diphenyl ethers.

Field experiments were conducted with the recirculating sprayer (RCS) at Lincoln, Nebraska from 1974 through 1978. Different spray pressures, spray nozzles, and spray volumes with the RCS showed no significant differences in shattercane [Sorghum bicolor (L.) Moench] control or soybean [Glycine max (L.) Merr.] injury when herbicides were applied at three stages of weed growth. When shattercane was treated in a grain sorghum [Sorghum bicolor (L.) Moench] field, poor weed control and excessive crop injury occurred during treatment at the early growth stage as compared with treatments applied 2 weeks later. The final treatment date gave selective weed control in grain sorghum, but many of the shattercane heads had already developed viable seed. A weed-to-crop height differential of at least 45 cm resulted in maximum weed control with minimum crop injury. Common milkweed (Asclepias syriaca L.) control in soybeans varied considerably, but treatments giving over 80% control were glyphosate [N-(phosphonomethyl)glycine] at 1.1 to 4.5 kg/ha applied through the RCS. Other herbicides were less effective. Volunteer corn (Zea mays L.) was controlled selectively at 75 to 100% in soybeans with glyphosate or paraquat (1,1′-dimethyl-4,4′-bipyridinium ion) when applied through the RCS. Shattercane was controlled 95 to 100% in soybeans with glyphosate at 3.4 kg/ha. Unless spray drift and splash can be prevented when using the RCS, glyphosate and paraquat will not give selective control when applied to weeds growing in grain sorghum. Glyphosate applied through the RCS, however, can be a selective method of controlling weed escapes in soybeans because soybeans are not as sensitive to glyphosate as is sorghum.

The rate and final germination percentage and emergence of green foxtail [Setaria viridis (L.) Beauv.] was found to be very dependent on the soil temperature and moisture conditions at the time of seeding. In contrast, the germination and emergence of wheat (Triticum aestivum L. ‘Sinton’) was only slightly affected over the temperature and moisture range of the study. In laboratory studies, a temperature decrease from 20 to 15 C caused a 53- and 12-h delay in the time to reach 50% germination of green foxtail and wheat, respectively. Soil moisture was found to have a greater effect than soil temperature on green foxtail germination. At −5.3 bars water potential, the final germination percentage of green foxtail was less than at the higher water potentials. A further decrease in the water potential to −6.5 bars effectively reduced green foxtail germination to zero. Wheat germination was delayed only slightly, and the final germination percentage remained unchanged over the range of 0 to −15.3 bars water potential. In field studies, a soil temperature decrease from 22 to 14 C caused a 6-day delay in the time of 50% emergence of green foxtail, and the emergence of wheat was delayed only 1 day by a similar decrease in temperature. Field studies showed that when the soil was moist (0 to −4 bars) and soil temperatures were warm (20 to 25 C), green foxtail emerged within a few days of wheat. However, when the soil was dry (-4.0 to −6.5 bars) and soil temperatures were low (15 to 20 C), green foxtail emerged 7 to 14 days after wheat. The intensity of green foxtail competition was greater when green foxtail emerged within a few days of wheat than when green foxtail emerged 2 weeks after wheat.

Field studies of herbicidal antagonism were conducted in corn (Zea mays L.), soybeans (Glycine max L. Merr.), and on industrial sites. The addition of chlorbromuron [3-(4-bromo-3-chlorophenyl)-1-methoxy-1-methylurea], cyanazine {2-[[4-(chloro-6-(ethylamino)-s-triazin-2-yl] amino]-2-methylpropionitrile}, bifenox [methyl 5-(2,4-dichlorophenoxy)-2-nitrobenzoate], or atrazine [2-chloro-4-(ethylamino)-6-(isopropylamino)-s-triazine] wettable powder plus dicamba (3,6-dichloro-o-anisic acid) to glyphosate [N-(phosphonomethyl)glycine]-alachlor[2-chloro-2′,6′-diethyl-N-(methoxymethyl)acetanilide] combinations reduced the activity of glyphosate on quackgrass [Agropyron repens (L.) Beauv.], common dandelion (Taraxacum officinale Weber), and Canada thistle [Cirsium arvense (L.) Scop.] in no-till corn. Reduced weed control from antagonism resulted in decreased corn yields. Linuron [3-(3,4-dichlorophenyl)-1-methoxy-1-methylurea], chlorbromuron, or metribuzin [4-amino-6-tert-butyl-3-(methylthio)-as-triazin-5(4H)-one], when added to glyphosate, reduced the control of quackgrass but not that of Canada thistle, alfalfa, or common dandelion in soybeans. Antagonsim was not evident in annual weed species. In perennial weeds, the degree of antagonism was often reduced with increased dosages of glyphosate. The inclusion of terbacil (3-tert-butyl-5-chloro-6-methyluracil), bromacil (5-bromo-3-sec-butyl-6-methyluracil), and simazine [2-chloro-4,6-bis(ethylamino)-s-triazine] with glyphosate in mixes interfered with the control of smooth brome (Bromus inermis Leyss.), but not when applications of the residual herbicides were delayed. Quackgrass control was reduced when amitrole (3-amino-s-triazole) was mixed with glyphosate, or when applied separately.

Glyphosate [N-(phosphonomethyl)glycine] was applied at various stages of maturity to corn (Zea mays L. ‘Pioneer brand 3147’ and ‘Dekalb XL 394’), soybeans [Glycine max (L.) Merr. ‘Forrest’ and ‘Essex’], and johnsongrass [Sorghum halepense (L.) Pers.]. Glyphosate applied over-the-top of corn before the grain moisture level decreased to 30% (black layer will have been formed) caused various seed and subsequent progeny abnormalities. Depending on grain moisture level at the time of glyphosate application, seed weight was sometimes reduced and progeny seedling emergence, vigor, and weight were reduced. Also, abnormal seedlings, albino or straited, occurred. Glyphosate applied 2½ weeks or more before soybean maturity reduced seed weight, caused seed discoloration, and drastically reduced progeny seedling emergence, vigor, and weight. Glyphosate applied in September or early October controlled semimature johnsongrass. Later applications were less effective because of advanced senescence.

The field performance of amitrole (3-amino-s-triazole) formulations containing a number of additives was tested on bracken [Pteridium aquilinum (L.) Kuhn var. aquilinum]. All formulations that did not contain thiocyanate produced severe foliar scorching within 72 h of spraying. The following season, control was relatively poor (63 to 77% reductions in frond density). The formulation of amitrole with sodium iodide produced particularly severe scorching followed by a nonsignificant reduction in frond density (2%) the following season. Formulations containing ammonium thiocyanate (molar ratios of 1:0.5, 1:0.75, 1:1, and 1:1.25 amitrole:ammonium thiocyanate) greatly reduced this foliar scorching, and control the following season was of the order of 94 to 97% in all cases. Translocation was proposed as being the limiting factor in control, ammonium thiocyanate increasing translocation by preventing scorching and hence decreasing decomposition in the foliage. To investigate scorching further, a bioassay, which consisted of floating bracken leaflets on amitrole solutions, was developed. The commercial 1:1 molar ratio of amitrole:ammonium thiocyanate may not be optimum for all conditions. Other additives were tested and cyanide and hexacyanoferrate (II) were found to be very active scorch inhibitors.

Weed and cucumber (Cucumis sativus L. ‘Poinsett’) growth was studied where herbicides were soil-applied or applied to emerged weeds in the established crop. Cucumber yields were decreased at least 36% in 2 of 5 yr when weeds interfered for 4 weeks after emergence. Sequential applications of bensulide [O,O-diisopropyl phosphorodithioate S-ester with N-(2-mercaptoethyl)benzenesulfonamide] applied preplant and incorporated (PPI) + trifluralin (α,α,α-trifluoro-2,6-dinitro-N,N-dipropyl-p-toluidine) applied postemergence and incorporated (POI) were outstanding and controlled Japanese millet (Echinochloa crus-galli Link. var. frumentacea), Texas panicum (Panicum texanum Buckl.), Palmer amaranth (Amaranthus palmeri S. Wats.), and common purslane (Portulaca oleracea L.) without affecting the growth or yield of cucumber. Compared with bensulide, the selectivity of soil-incorporated butralin [4-(1,1-dimethylethyl)-N-(1-methylpropyl)-2,6-dinitrobenzenamine] with cucumber was limited. Laboratory bioassays showed that under warm field soil conditions, PPI applications of 1.7 kg/ha of butralin persisted more than 2 months, but were dissipated after 4 months. Ethalfluralin [N-ethyl-N-(2-methyl-2-propenyl)-2,6-dinitro-4-(trifluoromethyl)benzenamine] reduced cucumber yields only when it was soil-incorporated to the crop-seed depth at planting. Applications to soil of bensulide + DCPA (dimethyl tetrachloroterephthalate) stunted cucumber plants, reduced leaf area, increased leaf thickness, and caused higher near-infrared (0.75 to 1.34 μm) leaf reflectance.

Responses of soybeans [Glycine max (L.) Merr. ‘Bragg’] and red rice (Oryza sativa L.) to postemergence applications of mefluidide {N-[2,4-dimethyl-5-[[(trifluoromethyl)sulfonyl] amino] phenyl] acetamide} and bentazon [3-isopropyl-1H-2,1,3-benzothiadiazin-4(3H)-one 2,2-dioxide] alone and in combinations were evaluated in greenhouse studies. Soybeans were tolerant to bentazon up to the highest tested rate of 2.0 kg/ha, but their height was reduced by mefluidide at 1.0 kg/ha. Soybeans were not injured by any tested combination of the two herbicides. Bentazon applied alone at rates of 5.0 kg/ha and above had a significant effect on red rice injury. Mefluidide applied at rates above 0.25 kg/ha severely reduced height and fresh weight of red rice. When these two herbicides were combined, a synergistic reaction occurred, and the combination was much more effective in controlling red rice than was either herbicide applied alone. The addition of a surfactant significantly increased the activity of the combination on red rice up to 0.25 kg/ha of mefluidide and at all rates of bentazon.

Competition of green foxtail [Setaria viridis (L.) Beauv.] was studied in a semi-dwarf wheat (Triticum aestivum L. ‘Norquay’) and in two normal-height wheats (‘Napayo’ and ‘Sinton’) from 1975 through 1978. Green foxtail suppressed wheat growth as well as grain yield. Tiller number, leaf area, and dry weight of wheat were reduced. Green foxtail was more competitive in the semi-dwarf variety than in either normal height variety. The intensity of green foxtail competition could not be determined by density alone. In 1975, as few as 100 green foxtail plants/m2 significantly reduced yield of Napayo and Norquay wheat by 21 and 44%, respectively. In 1977, however, 1600 green foxtail plants/m2 did not reduce the yield of Sinton wheat significantly. The intensity of green foxtail competition was highly variable from one date of seeding to the next, but there was no correlation between the level of green foxtail competition and the date of seeding. Soil temperature and moisture at the time of seeding and early growth are thought to affect green foxtail competition in wheat critically.

Field and greenhouse experiments were conducted to observe and measure the phytotoxicity of glyphosate [N-(phosphonomethyl)glycine] to potatoes (Solanum tuberosum L. ‘Russet Burbank’) applied at different stages of growth and to determine if glyphosate was translocated into the original mother tuber (seed piece) prior to initiation of daughter tubers. Glyphosate injury symptoms appeared 1 day after treatment and at least 50% of the foliage was necrotic within 7 days. Significant decreases in plant height, shoot and root dry matter content, and daughter tuber production were recorded at the 0.28- and 0.56-kg/ha rates. Higher rates of 1.12 and 2.24 kg/ha did not give further significant reductions. Maximum levels of 14C activity accumulated in all plant parts 4 days after application of the 14 C-glyphosate. The 14 C-glyphosate accumulated primarily in the apical meristem and roots. Extremely low levels of 14 C activity were detected in the mother tuber regardless of plant age when treated. Phytotoxic effects on the eyes of the mother tuber increased in severity with increasing rates of glyphosate as evidenced by abnormal sprouting.

The distribution patterns of several herbicide formulations sprayed on adaxial leaf surfaces were determined using scanning electron microscopy coupled with cathodoluminescence and x-ray microanalysis. The sodium and amine salts of MCPA {[(4-chloro-o-tolyl) oxy] acetic acid} sprayed on sugar beet (Beta vulgaris L.) leaves appeared as discrete deposits above the anticlinal cell walls that represented the location of spray drops that adhered to the leaf. When the sodium salt was applied to bermudagrass [Cynodon dactylon (L.) Pers.], the pattern of distribution was the same; however, each deposit was significantly smaller. The iso-octyl ester of MCPA coalesced into numerous, small, thick deposits on the cuticle of sugar beet leaves. The distribution of a wettable powder formulation of atrazine [2-chloro-4-(ethylamino)-6-(isopropylamino-s-triazine] appeared as uniform deposits over the anticlinal and periclinal cell walls that represented the location of aqueous spray drops after application. When a flowable formulation of atrazine was applied, there was a significant preferential accumulation of the herbicide at the edges of the separate deposits. One commercial formulation of propanil (3′,4′-dichloropropionanilide) yielded deposits that were crystalline, one that was partially crystalline, and one that was noncrystalline.

The uptake and translocation of fluridone {1-methyl-3-phenyl-5-[3-(trifluoromethyl)phenyl]-4(1H)-pyridinone} were examined in sago pondweed (Potamogeton pectinatus L.) and Richardson pondweed [Potamogeton richardsonii (Ar. Benn.) Rydb.]. Root and shoot tissues of both species were isolated from each other with wax barriers and treated individually with 1.0 ppm 14C-fluridone. Both tissues bioconcentrated fluridone, but the amount absorbed represented 1% or less of the total herbicide available. Limited root-to-shoot translocation occurred, but shoot-to-root transport was negligible. In contrast to fluridone, highly mobile glyphosate [N-(phosphonomethyl) glycine] translocated from the shoots to the roots in sago pondweed. No metabolism of fluridone was detected in sago pondweed.

The fate of foliarly applied 14C diclofop-methyl {methyl 2-[4-(2,3-dichlorophenoxy)phenoxy] propanoate} was determined in intact plants of barnyardgrass [Echinochloa crus-galli (L.) Beauv.], a susceptible (S) grass; proso millet (Panicum miliaceum L.), a moderately susceptible (MS) grass; longspine sandbur [Cenchrus longispinus (Hack.) Fern.], a tolerant (T) grass; soybean [Glycine max (L.) Merr. ‘Hark’], and cucumber (Cucumis sativus L. ‘Green Star’), both T broadleaf plants, 1,3, and 5 days after treatment (DAT). Diclofop-methyl accounted for 94% of the radioactivity washed from the leaf surfaces of all species. Plant extracts contained diclofop-methyl, diclofop, and water-soluble conjugates. Barnyardgrass (S), proso millet (MS), and soybean (T) had 75, 68, and 66% of the extracted radioactivity as diclofop and diclofop-methyl. Longspine sandbur (T) and cucumber (T) had 71 and 84% of the extracted radioactivity as water-soluble conjugates. Acid hydrolysis of the water-soluble conjugates yielded 84 and 71% diclofop in barnyardgrass (S) and proso millet (MS). Cucumber (T), soybean (T), and longspine sandbur (T) had 43, 23, and 25% ring-OH diclofop. Alkaline hydrolysis of the non-extracted plant residue yielded diclofop as the major component in all species.